Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle

The effect of freezing rate, temperature and storage time of Anisakis L3 infecting fish mince in controlled conditions were studied in terms of larval viability and parasite antigen release. In order to determine the potential infectivity of larvae that might accidentally survive a freezing process,...

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Autores: Sánchez Alonso, Isabel, Carballeda-Sangiao, Noelia, González Muñoz, Miguel, Navas, Alfonso, Arcos, Susana C., Mendizábal, Angel, Cuesta, Francisco Javier, Careche, Mercedes
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2020
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/219383
Acesso em linha:http://hdl.handle.net/10261/219383
Access Level:Acceso aberto
Palavra-chave:Anisakis
Respiratory analysis
Fish muscle
Survival in gastric juice
Freezing
Allergens
Agar penetrative ability
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spelling Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscleSánchez Alonso, IsabelCarballeda-Sangiao, NoeliaGonzález Muñoz, MiguelNavas, AlfonsoArcos, Susana C.Mendizábal, AngelCuesta, Francisco JavierCareche, MercedesAnisakisRespiratory analysisFish muscleSurvival in gastric juiceFreezingAllergensAgar penetrative abilityThe effect of freezing rate, temperature and storage time of Anisakis L3 infecting fish mince in controlled conditions were studied in terms of larval viability and parasite antigen release. In order to determine the potential infectivity of larvae that might accidentally survive a freezing process, their agar penetration ability, survival in artificial gastric juice, and respiratory control were analysed in infected hake muscle in conditions that produced various survival rates. For that a series of experiments was conducted with 10–30 L3 per 75 g of mince, frozen and stored to provide a variety of freezing kinetic conditions and storage times. The results highlight the importance of a number of kinetic parameters in the survival of Anisakis larvae, not only final temperatures or storage times. High freezing rates, preferred in order to maintain optimum fish eating quality, are able to kill Anisakis larvae faster, but may cause a higher release of antigens to the surrounding medium. The characteristics of the surviving L3 were impaired as compared to the controls. No relationship between the latter and the percentage of viability due to different freezing conditions was found, which suggest that any larva which shows any sign of movement after freezing should be treated as equally infective.This work was financed by the European Union's Seventh Framework Programme for Research, Technological Development and Demonstration under Grant Agreement 312068, EU PARASITE and the project ANIRISK (AGL2015-68248-C2) is cofunded by the Spanish Ministry of Economy and Competitivenes (MINECO) and FEDER funds from EU.Peer reviewedElsevierEuropean CommissionMinisterio de Economía y Competitividad (España)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/219383reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/EC/FP7/312068info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68248-C2https://doi.org/10.1016/j.foodcont.2020.107373Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2193832026-05-22T06:33:51Z
dc.title.none.fl_str_mv Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
title Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
spellingShingle Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
Sánchez Alonso, Isabel
Anisakis
Respiratory analysis
Fish muscle
Survival in gastric juice
Freezing
Allergens
Agar penetrative ability
title_short Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
title_full Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
title_fullStr Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
title_full_unstemmed Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
title_sort Freezing kinetic parameters influence allergenic and infective potential of Anisakis simplex L3 present in fish muscle
dc.creator.none.fl_str_mv Sánchez Alonso, Isabel
Carballeda-Sangiao, Noelia
González Muñoz, Miguel
Navas, Alfonso
Arcos, Susana C.
Mendizábal, Angel
Cuesta, Francisco Javier
Careche, Mercedes
author Sánchez Alonso, Isabel
author_facet Sánchez Alonso, Isabel
Carballeda-Sangiao, Noelia
González Muñoz, Miguel
Navas, Alfonso
Arcos, Susana C.
Mendizábal, Angel
Cuesta, Francisco Javier
Careche, Mercedes
author_role author
author2 Carballeda-Sangiao, Noelia
González Muñoz, Miguel
Navas, Alfonso
Arcos, Susana C.
Mendizábal, Angel
Cuesta, Francisco Javier
Careche, Mercedes
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Ministerio de Economía y Competitividad (España)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Anisakis
Respiratory analysis
Fish muscle
Survival in gastric juice
Freezing
Allergens
Agar penetrative ability
topic Anisakis
Respiratory analysis
Fish muscle
Survival in gastric juice
Freezing
Allergens
Agar penetrative ability
description The effect of freezing rate, temperature and storage time of Anisakis L3 infecting fish mince in controlled conditions were studied in terms of larval viability and parasite antigen release. In order to determine the potential infectivity of larvae that might accidentally survive a freezing process, their agar penetration ability, survival in artificial gastric juice, and respiratory control were analysed in infected hake muscle in conditions that produced various survival rates. For that a series of experiments was conducted with 10–30 L3 per 75 g of mince, frozen and stored to provide a variety of freezing kinetic conditions and storage times. The results highlight the importance of a number of kinetic parameters in the survival of Anisakis larvae, not only final temperatures or storage times. High freezing rates, preferred in order to maintain optimum fish eating quality, are able to kill Anisakis larvae faster, but may cause a higher release of antigens to the surrounding medium. The characteristics of the surviving L3 were impaired as compared to the controls. No relationship between the latter and the percentage of viability due to different freezing conditions was found, which suggest that any larva which shows any sign of movement after freezing should be treated as equally infective.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/219383
url http://hdl.handle.net/10261/219383
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/EC/FP7/312068
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-68248-C2
https://doi.org/10.1016/j.foodcont.2020.107373
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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