Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum

https://link.springer.com/article/10.1007/s00253-022-12182-9

Detalles Bibliográficos
Autores: Cardoza, Rosa E., McCormick, Susan P., Izquierdo-Bueno, Inmaculada, Martínez-Reyes, Natalia, Lindo, Laura, Brown, Daren W., Collado, Isidro G., Proctor, Robert H., Gutiérrez , Santiago 1965-
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Institución:Universidad de León
Repositorio:BULERIA. Repositorio Institucional de la Universidad de León
OAI Identifier:oai:buleria.unileon.es:10612/15149
Acceso en línea:http://hdl.handle.net/10612/15149
Access Level:acceso abierto
Palabra clave:Biología
Aspinolides
Trichoderma 
Trichothecenes
Metabolomics 
Gene clusters
Comparative genomics
2415 Biología Molecular
2414 Microbiología
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oai_identifier_str oai:buleria.unileon.es:10612/15149
network_acronym_str ES
network_name_str España
repository_id_str
spelling Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceumCardoza, Rosa E.McCormick, Susan P.Izquierdo-Bueno, InmaculadaMartínez-Reyes, NataliaLindo, LauraBrown, Daren W.Collado, Isidro G.Proctor, Robert H.Gutiérrez , Santiago 1965-BiologíaAspinolidesTrichoderma TrichothecenesMetabolomics Gene clustersComparative genomics2415 Biología Molecular2414 Microbiologíahttps://link.springer.com/article/10.1007/s00253-022-12182-9[EN] The fungus Trichoderma arundinaceum exhibits biological control activity against crop diseases caused by other fungi. Two mechanisms that likely contribute to this activity are upregulation of plant defenses and production of two types of antifungal secondary metabolites: the sesquiterpenoid harzianum A (HA) and the polyketide-derived aspinolides. The goal of the current study was to identify aspinolide biosynthetic genes as part of an effort to understand how these metabolites contribute to the biological control activity of T. arundinaceum. Comparative genomics identified two polyketide synthase genes (asp1 and asp2) that occur in T. arundinaceum and Aspergillus ochraceus, which also produces aspinolides. Gene deletion and biochemical analyses in T. arundinaceum indicated that both genes are required for aspinolide production: asp2 for formation of a 10-member lactone ring and asp1 for formation of a butenoyl subsituent at position 8 of the lactone ring. Gene expression and comparative genomics analyses indicated that asp1 and asp2 are located within a gene cluster that occurs in both T. arundinaceum and A. ochraceus. A survey of genome sequences representing 35 phylogenetically diverse Trichoderma species revealed that intact homologs of the cluster occurred in only two other species, which also produced aspinolides. An asp2 mutant inhibited fungal growth more than the wild type, but an asp1 mutant did not, and the greater inhibition by the asp2 mutant coincided with increased HA production. These findings indicate that asp1 and asp2 are aspinolide biosynthetic genes and that loss of either aspinolide or HA production in T. arundinaceum can be accompanied by increased production of the other metabolite(s).SIPublicación en abierto financiada por el Consorcio de Bibliotecas Universitarias de Castilla y León (BUCLE), con cargo al Programa Operativo 2014ES16RFOP009 FEDER 2014-2020 DE CASTILLA Y LEÓN, Actuación:20007-CL - Apoyo Consorcio BUCLESpringerMicrobiologiaFacultad de Ciencias Biologicas y Ambientales2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10612/15149reponame:BULERIA. Repositorio Institucional de la Universidad de Leóninstname:Universidad de LeónInglésinfo: eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-099600-B-I00/AISLAMIENTO DE CEPAS DE TRICHODERMA PRODUCTORAS DE TRICHOTECENOS A PARTIR DE CULTIVOS DE JUDIA Y ESTUDIO DE SU EFECTO EN LA DEFENSA DE LA PLANTA FRENTE A ENFERMEDADES FUNGICASinfo: eu-repo/grantAgreement/MCIN/ AEI/10.13039/501100011033http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:buleria.unileon.es:10612/151492026-06-24T12:43:27Z
dc.title.none.fl_str_mv Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
title Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
spellingShingle Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
Cardoza, Rosa E.
Biología
Aspinolides
Trichoderma 
Trichothecenes
Metabolomics 
Gene clusters
Comparative genomics
2415 Biología Molecular
2414 Microbiología
title_short Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
title_full Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
title_fullStr Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
title_full_unstemmed Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
title_sort Identification of polyketide synthase genes required for aspinolide biosynthesis in Trichoderma arundinaceum
dc.creator.none.fl_str_mv Cardoza, Rosa E.
McCormick, Susan P.
Izquierdo-Bueno, Inmaculada
Martínez-Reyes, Natalia
Lindo, Laura
Brown, Daren W.
Collado, Isidro G.
Proctor, Robert H.
Gutiérrez , Santiago 1965-
author Cardoza, Rosa E.
author_facet Cardoza, Rosa E.
McCormick, Susan P.
Izquierdo-Bueno, Inmaculada
Martínez-Reyes, Natalia
Lindo, Laura
Brown, Daren W.
Collado, Isidro G.
Proctor, Robert H.
Gutiérrez , Santiago 1965-
author_role author
author2 McCormick, Susan P.
Izquierdo-Bueno, Inmaculada
Martínez-Reyes, Natalia
Lindo, Laura
Brown, Daren W.
Collado, Isidro G.
Proctor, Robert H.
Gutiérrez , Santiago 1965-
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Microbiologia
Facultad de Ciencias Biologicas y Ambientales
dc.subject.none.fl_str_mv Biología
Aspinolides
Trichoderma 
Trichothecenes
Metabolomics 
Gene clusters
Comparative genomics
2415 Biología Molecular
2414 Microbiología
topic Biología
Aspinolides
Trichoderma 
Trichothecenes
Metabolomics 
Gene clusters
Comparative genomics
2415 Biología Molecular
2414 Microbiología
description https://link.springer.com/article/10.1007/s00253-022-12182-9
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10612/15149
url http://hdl.handle.net/10612/15149
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info: eu-repo/grantAgreement/AEI/Programa Estatal de I+D+i Orientada a los Retos de la Sociedad/RTI2018-099600-B-I00/AISLAMIENTO DE CEPAS DE TRICHODERMA PRODUCTORAS DE TRICHOTECENOS A PARTIR DE CULTIVOS DE JUDIA Y ESTUDIO DE SU EFECTO EN LA DEFENSA DE LA PLANTA FRENTE A ENFERMEDADES FUNGICAS
info: eu-repo/grantAgreement/MCIN/ AEI/10.13039/501100011033
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer
publisher.none.fl_str_mv Springer
dc.source.none.fl_str_mv reponame:BULERIA. Repositorio Institucional de la Universidad de León
instname:Universidad de León
instname_str Universidad de León
reponame_str BULERIA. Repositorio Institucional de la Universidad de León
collection BULERIA. Repositorio Institucional de la Universidad de León
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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