Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis

Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granule...

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Authors: Gutiérrez-Beltrán, Emilio, Elander, Pernilla H., Dalman, Kerstin, Dayhoff II, Guy W., Moschou, Panagiotis N., Crespo, José L., Bozhkov, Peter V., Uversky, Vladimir
Format: article
Status:Published version
Publication Date:2021
Country:España
Institution:Consejo Superior de Investigaciones Científicas (CSIC)
Repository:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/247446
Online Access:http://hdl.handle.net/10261/247446
Access Level:Open access
Keyword:Heat stress
Intrinsically disordered regions
SnRK1/SNF1/AMPK
Stress granules
Tudor staphylococcal nuclease
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spelling Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in ArabidopsisGutiérrez-Beltrán, EmilioElander, Pernilla H.Dalman, KerstinDayhoff II, Guy W.Moschou, Panagiotis N.Crespo, José L.Bozhkov, Peter V.Dalman, KerstinUversky, VladimirHeat stressIntrinsically disordered regionsSnRK1/SNF1/AMPKStress granulesTudor staphylococcal nucleaseTudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plantspecific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signallingPeer reviewedEMBO PressMinisterio de Economía y Competitividad (España)Gutiérrez-Beltrán, Emilio [0000-0001-7978-3164]Dalman, Kerstin [0000-0002-9919-9149]Moschou, Panagiotis N. [0000-0001-7212-0595]Uversky, Vladimir [0000-0002-4037-5857]Crespo, José L. [0000-0003-3514-1025]Bozhkov, Peter V. [/0000-0002-8819-3884]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/247446reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PGC2018-099048-B-100Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2474462026-05-22T06:33:51Z
dc.title.none.fl_str_mv Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
title Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
spellingShingle Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
Gutiérrez-Beltrán, Emilio
Heat stress
Intrinsically disordered regions
SnRK1/SNF1/AMPK
Stress granules
Tudor staphylococcal nuclease
title_short Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
title_full Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
title_fullStr Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
title_full_unstemmed Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
title_sort Tudor staphylococcal nuclease is a docking platform for stress granule components and is essential for SnRK1 activation in Arabidopsis
dc.creator.none.fl_str_mv Gutiérrez-Beltrán, Emilio
Elander, Pernilla H.
Dalman, Kerstin
Dayhoff II, Guy W.
Moschou, Panagiotis N.
Crespo, José L.
Bozhkov, Peter V.
Dalman, Kerstin
Uversky, Vladimir
author Gutiérrez-Beltrán, Emilio
author_facet Gutiérrez-Beltrán, Emilio
Elander, Pernilla H.
Dalman, Kerstin
Dayhoff II, Guy W.
Moschou, Panagiotis N.
Crespo, José L.
Bozhkov, Peter V.
Uversky, Vladimir
author_role author
author2 Elander, Pernilla H.
Dalman, Kerstin
Dayhoff II, Guy W.
Moschou, Panagiotis N.
Crespo, José L.
Bozhkov, Peter V.
Uversky, Vladimir
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía y Competitividad (España)
Gutiérrez-Beltrán, Emilio [0000-0001-7978-3164]
Dalman, Kerstin [0000-0002-9919-9149]
Moschou, Panagiotis N. [0000-0001-7212-0595]
Uversky, Vladimir [0000-0002-4037-5857]
Crespo, José L. [0000-0003-3514-1025]
Bozhkov, Peter V. [/0000-0002-8819-3884]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Heat stress
Intrinsically disordered regions
SnRK1/SNF1/AMPK
Stress granules
Tudor staphylococcal nuclease
topic Heat stress
Intrinsically disordered regions
SnRK1/SNF1/AMPK
Stress granules
Tudor staphylococcal nuclease
description Tudor staphylococcal nuclease (TSN; also known as Tudor-SN, p100, or SND1) is a multifunctional, evolutionarily conserved regulator of gene expression, exhibiting cytoprotective activity in animals and plants and oncogenic activity in mammals. During stress, TSN stably associates with stress granules (SGs), in a poorly understood process. Here, we show that in the model plant Arabidopsis thaliana, TSN is an intrinsically disordered protein (IDP) acting as a scaffold for a large pool of other IDPs, enriched for conserved stress granule components as well as novel or plantspecific SG-localized proteins. While approximately 30% of TSN interactors are recruited to stress granules de novo upon stress perception, 70% form a protein–protein interaction network present before the onset of stress. Finally, we demonstrate that TSN and stress granule formation promote heat-induced activation of the evolutionarily conserved energy-sensing SNF1-related protein kinase 1 (SnRK1), the plant orthologue of mammalian AMP-activated protein kinase (AMPK). Our results establish TSN as a docking platform for stress granule proteins, with an important role in stress signalling
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/247446
url http://hdl.handle.net/10261/247446
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/PGC2018-099048-B-100
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv EMBO Press
publisher.none.fl_str_mv EMBO Press
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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