The role of Serratia marcescens porins in antibiotic resistance

The outer membrane permeability of Serratia marcescens was studied by comparing porin-deficient mutants with their parental strains. Omp1-deficient strains were selected by moxalactam resistance, whereas mutants lacking the Omp2 porin were obtained by experimental infection with the SMP2 phage, whos...

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Authors: Ruiz España, Neus, Montero Barrientos, Ma. Teresa, Borrell Hernández, Jordi, Viñas, Miquel
Format: article
Status:Published version
Publication Date:2003
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/54973
Online Access:https://hdl.handle.net/2445/54973
Access Level:Open access
Keyword:Resistència als medicaments
Antibiòtics
Medicaments antibacterians
Serratia marcescens
Drug resistance
Antibiotics
Antibacterial agents
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spelling The role of Serratia marcescens porins in antibiotic resistanceRuiz España, NeusMontero Barrientos, Ma. TeresaBorrell Hernández, JordiViñas, MiquelResistència als medicamentsAntibiòticsMedicaments antibacteriansSerratia marcescensDrug resistanceAntibioticsAntibacterial agentsSerratia marcescensThe outer membrane permeability of Serratia marcescens was studied by comparing porin-deficient mutants with their parental strains. Omp1-deficient strains were selected by moxalactam resistance, whereas mutants lacking the Omp2 porin were obtained by experimental infection with the SMP2 phage, whose primary receptor is the Omp2 porin. The role of porins was demonstrated in quinolone accumulation assays, where semi-quantitative differences in accumulation were observed. Permeability coefficients to cephaloridine of Omp1 mutants were determined and compared with those of the parental strain. The clinical isolates S. marcescens HCPR1 and 866 showed 30- to 200-fold reduced permeability coefficients when Omp1 porin was absentMary Ann Liebert, Inc.2003info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/54973Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1089/107662903322286463Microbial Drug Resistance, 2003, vol. 9, num. 3, p. 257-264http://dx.doi.org/10.1089/107662903322286463(c) Mary Ann Liebert, Inc., 2003info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/549732026-05-27T06:46:51Z
dc.title.none.fl_str_mv The role of Serratia marcescens porins in antibiotic resistance
title The role of Serratia marcescens porins in antibiotic resistance
spellingShingle The role of Serratia marcescens porins in antibiotic resistance
Ruiz España, Neus
Resistència als medicaments
Antibiòtics
Medicaments antibacterians
Serratia marcescens
Drug resistance
Antibiotics
Antibacterial agents
Serratia marcescens
title_short The role of Serratia marcescens porins in antibiotic resistance
title_full The role of Serratia marcescens porins in antibiotic resistance
title_fullStr The role of Serratia marcescens porins in antibiotic resistance
title_full_unstemmed The role of Serratia marcescens porins in antibiotic resistance
title_sort The role of Serratia marcescens porins in antibiotic resistance
dc.creator.none.fl_str_mv Ruiz España, Neus
Montero Barrientos, Ma. Teresa
Borrell Hernández, Jordi
Viñas, Miquel
author Ruiz España, Neus
author_facet Ruiz España, Neus
Montero Barrientos, Ma. Teresa
Borrell Hernández, Jordi
Viñas, Miquel
author_role author
author2 Montero Barrientos, Ma. Teresa
Borrell Hernández, Jordi
Viñas, Miquel
author2_role author
author
author
dc.subject.none.fl_str_mv Resistència als medicaments
Antibiòtics
Medicaments antibacterians
Serratia marcescens
Drug resistance
Antibiotics
Antibacterial agents
Serratia marcescens
topic Resistència als medicaments
Antibiòtics
Medicaments antibacterians
Serratia marcescens
Drug resistance
Antibiotics
Antibacterial agents
Serratia marcescens
description The outer membrane permeability of Serratia marcescens was studied by comparing porin-deficient mutants with their parental strains. Omp1-deficient strains were selected by moxalactam resistance, whereas mutants lacking the Omp2 porin were obtained by experimental infection with the SMP2 phage, whose primary receptor is the Omp2 porin. The role of porins was demonstrated in quinolone accumulation assays, where semi-quantitative differences in accumulation were observed. Permeability coefficients to cephaloridine of Omp1 mutants were determined and compared with those of the parental strain. The clinical isolates S. marcescens HCPR1 and 866 showed 30- to 200-fold reduced permeability coefficients when Omp1 porin was absent
publishDate 2003
dc.date.none.fl_str_mv 2003
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/54973
url https://hdl.handle.net/2445/54973
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1089/107662903322286463
Microbial Drug Resistance, 2003, vol. 9, num. 3, p. 257-264
http://dx.doi.org/10.1089/107662903322286463
dc.rights.none.fl_str_mv (c) Mary Ann Liebert, Inc., 2003
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Mary Ann Liebert, Inc., 2003
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc.
publisher.none.fl_str_mv Mary Ann Liebert, Inc.
dc.source.none.fl_str_mv Articles publicats en revistes (Farmàcia, Tecnologia Farmacèutica i Fisicoquímica)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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