Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography

In this work, we study the effect of different variables affecting elution profile distortion on the enantiomeric resolution eventually achievable when working with on-line coupled liquid chromatography to gas chromatography (LC-GC). Specifically, the proposed configuration combines achiral reversed...

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Autores: Martínez, Rosa M., Barba González-Albo, Carmen, Robredo, Sergio, Herraiz, Marta
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2019
País:España
Institución:Universidad Pública de Navarra
Repositorio:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
OAI Identifier:oai:academica-e.unavarra.es:2454/36556
Acceso en línea:https://hdl.handle.net/2454/36556
Access Level:acceso abierto
Palabra clave:Chiral analysis
Enantiodifferentiation
Enantio‐multidimensional chromatography
On‐line coupling LC‐GC
Peak shape distortion
Viscous fingering
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spelling Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatographyMartínez, Rosa M.Barba González-Albo, CarmenRobredo, SergioHerraiz, MartaChiral analysisEnantiodifferentiationEnantio‐multidimensional chromatographyOn‐line coupling LC‐GCPeak shape distortionViscous fingeringIn this work, we study the effect of different variables affecting elution profile distortion on the enantiomeric resolution eventually achievable when working with on-line coupled liquid chromatography to gas chromatography (LC-GC). Specifically, the proposed configuration combines achiral reversed-phase liquid chromatography (RPLC) and chiral gas chromatography (enantio-GC), with heptakis-(2,3,6-tri-O-methyl)-beta-cyclodextrin as enantioselective stationary phase to analyse target fractions transferred (from LC to GC) via the through oven transfer adsorption desorption (TOTAD) interface. The high degree of orthogonality resulting from the combination of two chromatographic columns having very different separation mechanisms (and also requiring mobile phases in distinct physical states), as well as integration of the sample preparation step in the first dimension of the system, significantly contributed to exploit the performance of the proposed two-dimensional approach. Occasional adverse effects, which may result in severe peak distortions during LC-GC analysis and could be explained by flow instabilities due to viscous fingering, are circumvented by using the outstanding capacity of the TOTAD interface for achieving effective elimination of the eluent arriving from the LC preseparation.Spanish Ministry of Economy and Competitiveness, Grant/Award Numbers: IPT‐010000‐2010‐017 and DEP2012‐35225WileyAgronomía, Biotecnología y AlimentaciónAgronomia, Bioteknologia eta Elikadura2019info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2454/36556reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarrainstname:Universidad Pública de NavarraInglésinfo:eu-repo/grantAgreement/ES/6PN/DEP2012‐35225© 2019 Wiley Periodicals, Inc.info:eu-repo/semantics/openAccessoai:academica-e.unavarra.es:2454/365562026-06-17T12:41:47Z
dc.title.none.fl_str_mv Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
title Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
spellingShingle Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
Martínez, Rosa M.
Chiral analysis
Enantiodifferentiation
Enantio‐multidimensional chromatography
On‐line coupling LC‐GC
Peak shape distortion
Viscous fingering
title_short Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
title_full Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
title_fullStr Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
title_full_unstemmed Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
title_sort Improving enantiomeric resolutions by avoiding peak distortion effects in on-line coupled liquid chromatography to gas chromatography
dc.creator.none.fl_str_mv Martínez, Rosa M.
Barba González-Albo, Carmen
Robredo, Sergio
Herraiz, Marta
author Martínez, Rosa M.
author_facet Martínez, Rosa M.
Barba González-Albo, Carmen
Robredo, Sergio
Herraiz, Marta
author_role author
author2 Barba González-Albo, Carmen
Robredo, Sergio
Herraiz, Marta
author2_role author
author
author
dc.contributor.none.fl_str_mv Agronomía, Biotecnología y Alimentación
Agronomia, Bioteknologia eta Elikadura
dc.subject.none.fl_str_mv Chiral analysis
Enantiodifferentiation
Enantio‐multidimensional chromatography
On‐line coupling LC‐GC
Peak shape distortion
Viscous fingering
topic Chiral analysis
Enantiodifferentiation
Enantio‐multidimensional chromatography
On‐line coupling LC‐GC
Peak shape distortion
Viscous fingering
description In this work, we study the effect of different variables affecting elution profile distortion on the enantiomeric resolution eventually achievable when working with on-line coupled liquid chromatography to gas chromatography (LC-GC). Specifically, the proposed configuration combines achiral reversed-phase liquid chromatography (RPLC) and chiral gas chromatography (enantio-GC), with heptakis-(2,3,6-tri-O-methyl)-beta-cyclodextrin as enantioselective stationary phase to analyse target fractions transferred (from LC to GC) via the through oven transfer adsorption desorption (TOTAD) interface. The high degree of orthogonality resulting from the combination of two chromatographic columns having very different separation mechanisms (and also requiring mobile phases in distinct physical states), as well as integration of the sample preparation step in the first dimension of the system, significantly contributed to exploit the performance of the proposed two-dimensional approach. Occasional adverse effects, which may result in severe peak distortions during LC-GC analysis and could be explained by flow instabilities due to viscous fingering, are circumvented by using the outstanding capacity of the TOTAD interface for achieving effective elimination of the eluent arriving from the LC preseparation.
publishDate 2019
dc.date.none.fl_str_mv 2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2454/36556
url https://hdl.handle.net/2454/36556
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/ES/6PN/DEP2012‐35225
dc.rights.none.fl_str_mv © 2019 Wiley Periodicals, Inc.
info:eu-repo/semantics/openAccess
rights_invalid_str_mv © 2019 Wiley Periodicals, Inc.
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Wiley
publisher.none.fl_str_mv Wiley
dc.source.none.fl_str_mv reponame:Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
instname:Universidad Pública de Navarra
instname_str Universidad Pública de Navarra
reponame_str Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
collection Academica-e. Repositorio Institucional de la Universidad Pública de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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