Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid

Colloid fluids supplemented with adequate combinations of coagulation factor concentrates with the capability to restore coagulation could be a desirable future treatment component in massive transfusion. Starting from a coagulation factor and blood cell-free albumin solution we added Prothrombin Co...

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Autores: Koller, Tobias|||0000-0002-6247-0193, Kinast, Nadia, Guilarte Castellanos, Andres, Pérez García, Sergio|||0000-0003-4242-0858, Paniagua, Pilar|||0000-0002-0215-1561, Vintro, Xavi León|||0000-0002-9673-5786, Arranz, Jose Mateo, Vilalta, Noelia|||0000-0002-3961-3563, García, Ma. Victòria Moral, Moreno-Castaño, Ana Belén|||0000-0003-0338-7514, Aznar-Salatti, Jose, Albaladejo, Gines Escolar, Diaz-Ricart, Maribel|||0000-0003-1122-0052
Formato: artículo
Fecha de publicación:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:239244
Acesso em linha:https://ddd.uab.cat/record/239244
https://dx.doi.org/urn:doi:10.1186/s13049-021-00867-5
Access Level:acceso abierto
Palavra-chave:Massive bleeding
Blood transfusion
Fibrinogen
Factor XIII
Prothrombin complex concentrate
Fluid therapy
Hemostatic resuscitation
Plasma substitutes
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spelling Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluidexperimental studies in thromboelastometryKoller, Tobias|||0000-0002-6247-0193Kinast, NadiaGuilarte Castellanos, AndresPérez García, Sergio|||0000-0003-4242-0858Paniagua, Pilar|||0000-0002-0215-1561Vintro, Xavi León|||0000-0002-9673-5786Arranz, Jose MateoVilalta, Noelia|||0000-0002-3961-3563García, Ma. Victòria MoralMoreno-Castaño, Ana Belén|||0000-0003-0338-7514Aznar-Salatti, JoseAlbaladejo, Gines EscolarDiaz-Ricart, Maribel|||0000-0003-1122-0052Massive bleedingBlood transfusionFibrinogenFactor XIIIProthrombin complex concentrateFluid therapyHemostatic resuscitationPlasma substitutesColloid fluids supplemented with adequate combinations of coagulation factor concentrates with the capability to restore coagulation could be a desirable future treatment component in massive transfusion. Starting from a coagulation factor and blood cell-free albumin solution we added Prothrombin Complex Concentrate, Fibrinogen Concentrate and Factor XIII in different combinations and concentrations to analyze their properties to restore thromboelastometry parameters without the use of plasma. Further analysis under the presence of platelets was performed for comparability to whole blood conditions. Albumin solutions enriched with Fibrinogen Concentrate, Factor XIII and Prothrombin Complex Concentrate at optimized concentrations show restoring coagulation potential. Prothrombin Complex Concentrate showed sufficient thrombin formation for inducing fibrinogen polymerization. The combination of Prothrombin Complex Concentrate and Fibrinogen Concentrate led to the formation of a stable in vitro fibrin clot. Fibrinogen and Factor XIII showed excellent capacity to improve fibrin clot firmness expressed as Amplitude at 10 min and Maximal Clot Firmness. Fibrinogen alone, or in combination with Factor XIII, was able to restore normal Amplitude at 10 min and Maximal Clot Firmness values. In the presence of platelets, the thromboelastometry surrogate parameter for thrombin generation (Clotting Time) improves and normalizes when compared to whole blood. Combinations of coagulation factor concentrates suspended in albumin solutions can restore thromboelastometry parameters in the absence of plasma. This kind of artificial colloid fluids with coagulation-restoring characteristics might offer new treatment alternatives for massive transfusion. Study registered at the institutional ethic committee "Institut de Recerca, Hospital Santa Creu i Sant Pau, with protocol number IIBSP-CFC-2013-165.Universitat Autònoma de Barcelona 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/239244https://dx.doi.org/urn:doi:10.1186/s13049-021-00867-5reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengInstituto de Salud Carlos III https://doi.org/10.13039/501100004587 DTS16-00133Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PIE15-00027Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI19-00888Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-675open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2392442026-06-06T12:50:31Z
dc.title.none.fl_str_mv Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
experimental studies in thromboelastometry
title Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
spellingShingle Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
Koller, Tobias|||0000-0002-6247-0193
Massive bleeding
Blood transfusion
Fibrinogen
Factor XIII
Prothrombin complex concentrate
Fluid therapy
Hemostatic resuscitation
Plasma substitutes
title_short Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
title_full Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
title_fullStr Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
title_full_unstemmed Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
title_sort Normalization of blood clotting characteristics using prothrombin complex concentrate, fibrinogen and FXIII in an albumin based fluid
dc.creator.none.fl_str_mv Koller, Tobias|||0000-0002-6247-0193
Kinast, Nadia
Guilarte Castellanos, Andres
Pérez García, Sergio|||0000-0003-4242-0858
Paniagua, Pilar|||0000-0002-0215-1561
Vintro, Xavi León|||0000-0002-9673-5786
Arranz, Jose Mateo
Vilalta, Noelia|||0000-0002-3961-3563
García, Ma. Victòria Moral
Moreno-Castaño, Ana Belén|||0000-0003-0338-7514
Aznar-Salatti, Jose
Albaladejo, Gines Escolar
Diaz-Ricart, Maribel|||0000-0003-1122-0052
author Koller, Tobias|||0000-0002-6247-0193
author_facet Koller, Tobias|||0000-0002-6247-0193
Kinast, Nadia
Guilarte Castellanos, Andres
Pérez García, Sergio|||0000-0003-4242-0858
Paniagua, Pilar|||0000-0002-0215-1561
Vintro, Xavi León|||0000-0002-9673-5786
Arranz, Jose Mateo
Vilalta, Noelia|||0000-0002-3961-3563
García, Ma. Victòria Moral
Moreno-Castaño, Ana Belén|||0000-0003-0338-7514
Aznar-Salatti, Jose
Albaladejo, Gines Escolar
Diaz-Ricart, Maribel|||0000-0003-1122-0052
author_role author
author2 Kinast, Nadia
Guilarte Castellanos, Andres
Pérez García, Sergio|||0000-0003-4242-0858
Paniagua, Pilar|||0000-0002-0215-1561
Vintro, Xavi León|||0000-0002-9673-5786
Arranz, Jose Mateo
Vilalta, Noelia|||0000-0002-3961-3563
García, Ma. Victòria Moral
Moreno-Castaño, Ana Belén|||0000-0003-0338-7514
Aznar-Salatti, Jose
Albaladejo, Gines Escolar
Diaz-Ricart, Maribel|||0000-0003-1122-0052
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona
dc.subject.none.fl_str_mv Massive bleeding
Blood transfusion
Fibrinogen
Factor XIII
Prothrombin complex concentrate
Fluid therapy
Hemostatic resuscitation
Plasma substitutes
topic Massive bleeding
Blood transfusion
Fibrinogen
Factor XIII
Prothrombin complex concentrate
Fluid therapy
Hemostatic resuscitation
Plasma substitutes
description Colloid fluids supplemented with adequate combinations of coagulation factor concentrates with the capability to restore coagulation could be a desirable future treatment component in massive transfusion. Starting from a coagulation factor and blood cell-free albumin solution we added Prothrombin Complex Concentrate, Fibrinogen Concentrate and Factor XIII in different combinations and concentrations to analyze their properties to restore thromboelastometry parameters without the use of plasma. Further analysis under the presence of platelets was performed for comparability to whole blood conditions. Albumin solutions enriched with Fibrinogen Concentrate, Factor XIII and Prothrombin Complex Concentrate at optimized concentrations show restoring coagulation potential. Prothrombin Complex Concentrate showed sufficient thrombin formation for inducing fibrinogen polymerization. The combination of Prothrombin Complex Concentrate and Fibrinogen Concentrate led to the formation of a stable in vitro fibrin clot. Fibrinogen and Factor XIII showed excellent capacity to improve fibrin clot firmness expressed as Amplitude at 10 min and Maximal Clot Firmness. Fibrinogen alone, or in combination with Factor XIII, was able to restore normal Amplitude at 10 min and Maximal Clot Firmness values. In the presence of platelets, the thromboelastometry surrogate parameter for thrombin generation (Clotting Time) improves and normalizes when compared to whole blood. Combinations of coagulation factor concentrates suspended in albumin solutions can restore thromboelastometry parameters in the absence of plasma. This kind of artificial colloid fluids with coagulation-restoring characteristics might offer new treatment alternatives for massive transfusion. Study registered at the institutional ethic committee "Institut de Recerca, Hospital Santa Creu i Sant Pau, with protocol number IIBSP-CFC-2013-165.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/239244
https://dx.doi.org/urn:doi:10.1186/s13049-021-00867-5
url https://ddd.uab.cat/record/239244
https://dx.doi.org/urn:doi:10.1186/s13049-021-00867-5
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 DTS16-00133
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PIE15-00027
Instituto de Salud Carlos III https://doi.org/10.13039/501100004587 PI19-00888
Agència de Gestió d'Ajuts Universitaris i de Recerca https://doi.org/10.13039/501100003030 2017/SGR-675
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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