Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light

Guanine-rich DNA sequences can fold into non-canonical nucleic acid structures called G-quadruplexes (G4s). These nanostructures have strong implications in many fields, from medical science to bottom-up nanotechnologies. As a result, ligands interacting with G4s have attracted great attention as ca...

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Detalhes bibliográficos
Autores: Libera, Valeria, Ripanti, Francesca, Petrillo, Caterina, Sacchetti, Francesco, Ramos-Soriano, Javier, Galán, M. Carmen, Schirò, Giorgio, Paciaroni, Alessandro, Comez, Lucia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/421017
Acesso em linha:http://hdl.handle.net/10261/421017
https://api.elsevier.com/content/abstract/scopus_id/85160377036
Access Level:acceso abierto
Palavra-chave:G-quadruplex
Circular dichroism
Photosensitive ligands
Singular value decomposition
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spelling Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible LightLibera, ValeriaRipanti, FrancescaPetrillo, CaterinaSacchetti, FrancescoRamos-Soriano, JavierGalán, M. CarmenSchirò, GiorgioPaciaroni, AlessandroComez, LuciaG-quadruplexCircular dichroismPhotosensitive ligandsSingular value decompositionGuanine-rich DNA sequences can fold into non-canonical nucleic acid structures called G-quadruplexes (G4s). These nanostructures have strong implications in many fields, from medical science to bottom-up nanotechnologies. As a result, ligands interacting with G4s have attracted great attention as candidates in medical therapies, molecular probe applications, and biosensing. In recent years, the use of G4-ligand complexes as photopharmacological targets has shown significant promise for developing novel therapeutic strategies and nanodevices. Here, we studied the possibility of manipulating the secondary structure of a human telomeric G4 sequence through the interaction with two photosensitive ligands, DTE and TMPyP4, whose response to visible light is different. The effect of these two ligands on G4 thermal unfolding was also considered, revealing the occurrence of peculiar multi-step melting pathways and the different attitudes of the two molecules on the quadruplex stabilization.This publication was supported by the CarESS project, D.R.no. 597, from the University of Perugia. G.S. was financially supported by the ANR-21-CE11-0036 “PhotoGene” grant. M.C.G., thanks to the European Research Council (ERC-COG: 648239) and EPSRC EP/S026215/1 J.R. acknowledges a MSCA fellowship (project 843720-BioNanoProbes).Peer reviewedMolecular Diversity Preservation InternationalUniversità degli Studi di PerugiaEuropean CommissionRipanti, Francesca [0000-0002-9505-740X]Sacchetti, Francesco [0000-0002-2705-5162]Ramos-Soriano, Javier [0000-0002-3054-0679]Galán, M. Carmen [0000-0001-7307-2871]Comez, Lucia [0000-0001-5160-6844]202620262023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/421017https://api.elsevier.com/content/abstract/scopus_id/85160377036reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésThe underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://www.mdpi.com/article/10.3390/ijms24109090/s1http://dx.doi.org/10.3390/ijms24109090Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4210172026-05-22T06:33:51Z
dc.title.none.fl_str_mv Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
title Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
spellingShingle Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
Libera, Valeria
G-quadruplex
Circular dichroism
Photosensitive ligands
Singular value decomposition
title_short Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
title_full Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
title_fullStr Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
title_full_unstemmed Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
title_sort Stability of Human Telomeric G-Quadruplexes Complexed with Photosensitive Ligands and Irradiated with Visible Light
dc.creator.none.fl_str_mv Libera, Valeria
Ripanti, Francesca
Petrillo, Caterina
Sacchetti, Francesco
Ramos-Soriano, Javier
Galán, M. Carmen
Schirò, Giorgio
Paciaroni, Alessandro
Comez, Lucia
author Libera, Valeria
author_facet Libera, Valeria
Ripanti, Francesca
Petrillo, Caterina
Sacchetti, Francesco
Ramos-Soriano, Javier
Galán, M. Carmen
Schirò, Giorgio
Paciaroni, Alessandro
Comez, Lucia
author_role author
author2 Ripanti, Francesca
Petrillo, Caterina
Sacchetti, Francesco
Ramos-Soriano, Javier
Galán, M. Carmen
Schirò, Giorgio
Paciaroni, Alessandro
Comez, Lucia
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Università degli Studi di Perugia
European Commission
Ripanti, Francesca [0000-0002-9505-740X]
Sacchetti, Francesco [0000-0002-2705-5162]
Ramos-Soriano, Javier [0000-0002-3054-0679]
Galán, M. Carmen [0000-0001-7307-2871]
Comez, Lucia [0000-0001-5160-6844]
dc.subject.none.fl_str_mv G-quadruplex
Circular dichroism
Photosensitive ligands
Singular value decomposition
topic G-quadruplex
Circular dichroism
Photosensitive ligands
Singular value decomposition
description Guanine-rich DNA sequences can fold into non-canonical nucleic acid structures called G-quadruplexes (G4s). These nanostructures have strong implications in many fields, from medical science to bottom-up nanotechnologies. As a result, ligands interacting with G4s have attracted great attention as candidates in medical therapies, molecular probe applications, and biosensing. In recent years, the use of G4-ligand complexes as photopharmacological targets has shown significant promise for developing novel therapeutic strategies and nanodevices. Here, we studied the possibility of manipulating the secondary structure of a human telomeric G4 sequence through the interaction with two photosensitive ligands, DTE and TMPyP4, whose response to visible light is different. The effect of these two ligands on G4 thermal unfolding was also considered, revealing the occurrence of peculiar multi-step melting pathways and the different attitudes of the two molecules on the quadruplex stabilization.
publishDate 2023
dc.date.none.fl_str_mv 2023
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/421017
https://api.elsevier.com/content/abstract/scopus_id/85160377036
url http://hdl.handle.net/10261/421017
https://api.elsevier.com/content/abstract/scopus_id/85160377036
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://www.mdpi.com/article/10.3390/ijms24109090/s1
http://dx.doi.org/10.3390/ijms24109090
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Molecular Diversity Preservation International
publisher.none.fl_str_mv Molecular Diversity Preservation International
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.228081

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