Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils

Noccaea brachypetala is a close relative of Noccaea caerulescens, a model plant species used in metal hyperaccumulation studies. In a previous survey in the Catalan Pyrenees, we found two occidental and two oriental N. brachypetala populations growing on non-metalliferous soils, with accumulated hig...

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Autores: Martos Arias, Soledad|||0000-0002-7345-8421, Busoms, Silvia|||0000-0002-9917-7118, Pérez-Martín, Laura|||0000-0001-6066-3939, Llugany, Mercè|||0000-0002-9940-0383, Cabot, Catalina, Poschenrieder, Charlotte|||0000-0002-3818-0874
Tipo de documento: artigo
Data de publicação:2021
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:255611
Acesso em linha:https://ddd.uab.cat/record/255611
https://dx.doi.org/urn:doi:10.3389/fmicb.2021.639997
Access Level:Acceso aberto
Palavra-chave:High-throughput sequencing (deep sequencing)
Noccaea brachypetala
Root-associated microbial communities
Zn/Cd hyperaccumulator
Non-metalliferous soil
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spelling Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous SoilsMartos Arias, Soledad|||0000-0002-7345-8421Busoms, Silvia|||0000-0002-9917-7118Pérez-Martín, Laura|||0000-0001-6066-3939Llugany, Mercè|||0000-0002-9940-0383Cabot, CatalinaPoschenrieder, Charlotte|||0000-0002-3818-0874High-throughput sequencing (deep sequencing)Noccaea brachypetalaRoot-associated microbial communitiesZn/Cd hyperaccumulatorNon-metalliferous soilNoccaea brachypetala is a close relative of Noccaea caerulescens, a model plant species used in metal hyperaccumulation studies. In a previous survey in the Catalan Pyrenees, we found two occidental and two oriental N. brachypetala populations growing on non-metalliferous soils, with accumulated high concentrations of Cd and Zn. Our hypothesis was that the microbiome companion of the plant roots may influence the ability of these plants to absorb metals. We performed high-throughput sequencing of the bacterial and fungal communities in the rhizosphere soil and rhizoplane fractions. The rhizobiomes and shoot ionomes of N. brachypetala plants were analyzed along with those from other non-hyperaccumulator Brassicaceae species found at the same sampling locations. The analyses revealed that microbiome richness and relative abundance tended to increase in N. brachypetala plants compared to non-hyperaccumulator species, regardless of plant location. We confirmed that the root compartment is a key factor in describing the community composition linked to the cohabiting Brassicaceae species, and the rhizoplane fraction contained the specific and rare taxa associated with each species. N. brachypetala plants harbored a similar relative abundance of fungi compared to the other plant hosts, but there was a notable reduction in some specific taxa. Additionally, we observed an enrichment in the hyperaccumulator rhizoplane of previously described metal-tolerant bacteria and bacteria involved in nitrogen cycling. The bacteria involved in the nitrogen cycle could contribute indirectly to the hyperaccumulator phenotype by improving soil quality and fertility. Our results indicate that N. brachypetala captures a particular prokaryotic community from the soil. This particular prokaryotic community may benefit the extraction of metal ions and/or improve plant nutrition. Our research identified satellite groups associated with the root niche of a hyperaccumulator plant that may assist in improving biological strategies in heavy metal remediation. 22021-01-0120212021-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/255611https://dx.doi.org/urn:doi:10.3389/fmicb.2021.639997reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104000RB-I00Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 BFU2016-75176-Ropen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2556112026-06-06T12:50:31Z
dc.title.none.fl_str_mv Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
title Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
spellingShingle Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
Martos Arias, Soledad|||0000-0002-7345-8421
High-throughput sequencing (deep sequencing)
Noccaea brachypetala
Root-associated microbial communities
Zn/Cd hyperaccumulator
Non-metalliferous soil
title_short Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
title_full Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
title_fullStr Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
title_full_unstemmed Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
title_sort Identifying the Specific Root Microbiome of the Hyperaccumulator Noccaea brachypetala Growing in Non-metalliferous Soils
dc.creator.none.fl_str_mv Martos Arias, Soledad|||0000-0002-7345-8421
Busoms, Silvia|||0000-0002-9917-7118
Pérez-Martín, Laura|||0000-0001-6066-3939
Llugany, Mercè|||0000-0002-9940-0383
Cabot, Catalina
Poschenrieder, Charlotte|||0000-0002-3818-0874
author Martos Arias, Soledad|||0000-0002-7345-8421
author_facet Martos Arias, Soledad|||0000-0002-7345-8421
Busoms, Silvia|||0000-0002-9917-7118
Pérez-Martín, Laura|||0000-0001-6066-3939
Llugany, Mercè|||0000-0002-9940-0383
Cabot, Catalina
Poschenrieder, Charlotte|||0000-0002-3818-0874
author_role author
author2 Busoms, Silvia|||0000-0002-9917-7118
Pérez-Martín, Laura|||0000-0001-6066-3939
Llugany, Mercè|||0000-0002-9940-0383
Cabot, Catalina
Poschenrieder, Charlotte|||0000-0002-3818-0874
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv High-throughput sequencing (deep sequencing)
Noccaea brachypetala
Root-associated microbial communities
Zn/Cd hyperaccumulator
Non-metalliferous soil
topic High-throughput sequencing (deep sequencing)
Noccaea brachypetala
Root-associated microbial communities
Zn/Cd hyperaccumulator
Non-metalliferous soil
description Noccaea brachypetala is a close relative of Noccaea caerulescens, a model plant species used in metal hyperaccumulation studies. In a previous survey in the Catalan Pyrenees, we found two occidental and two oriental N. brachypetala populations growing on non-metalliferous soils, with accumulated high concentrations of Cd and Zn. Our hypothesis was that the microbiome companion of the plant roots may influence the ability of these plants to absorb metals. We performed high-throughput sequencing of the bacterial and fungal communities in the rhizosphere soil and rhizoplane fractions. The rhizobiomes and shoot ionomes of N. brachypetala plants were analyzed along with those from other non-hyperaccumulator Brassicaceae species found at the same sampling locations. The analyses revealed that microbiome richness and relative abundance tended to increase in N. brachypetala plants compared to non-hyperaccumulator species, regardless of plant location. We confirmed that the root compartment is a key factor in describing the community composition linked to the cohabiting Brassicaceae species, and the rhizoplane fraction contained the specific and rare taxa associated with each species. N. brachypetala plants harbored a similar relative abundance of fungi compared to the other plant hosts, but there was a notable reduction in some specific taxa. Additionally, we observed an enrichment in the hyperaccumulator rhizoplane of previously described metal-tolerant bacteria and bacteria involved in nitrogen cycling. The bacteria involved in the nitrogen cycle could contribute indirectly to the hyperaccumulator phenotype by improving soil quality and fertility. Our results indicate that N. brachypetala captures a particular prokaryotic community from the soil. This particular prokaryotic community may benefit the extraction of metal ions and/or improve plant nutrition. Our research identified satellite groups associated with the root niche of a hyperaccumulator plant that may assist in improving biological strategies in heavy metal remediation.
publishDate 2021
dc.date.none.fl_str_mv 2
2021-01-01
2021
2021-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/255611
https://dx.doi.org/urn:doi:10.3389/fmicb.2021.639997
url https://ddd.uab.cat/record/255611
https://dx.doi.org/urn:doi:10.3389/fmicb.2021.639997
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PID2019-104000RB-I00
Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 BFU2016-75176-R
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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repository.mail.fl_str_mv
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