Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis

Background & Aims: Patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. We aimed at evaluating Bifidobacterium pseudocatenulatum CECT7765 effect on the host's macrophage func...

ver descrição completa

Detalhes bibliográficos
Autores: Moratalla A, CAPARROS, ESTHER, JUANOLA, ORIOL, Portune K, Puig-Kroger A, Estrada-Capetillo L, BELLOT, PABLO, Gomez-Hurtado I, PIÑERO, PAULA, GONZALEZ, JOSE MANUEL, Such J, Sanz Y, FRANCES, RUBÉN
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
Repositorio:r-FISABIO. Repositorio Institucional de Producción Científica
OAI Identifier:oai:fisabio.fundanetsuite.com:p2334
Acesso em linha:https://fisabio.portalinvestigacion.com/publicaciones/2334
Access Level:acceso abierto
Palavra-chave:Cirrhosis
Bifidobacterium pseudocatenulatum CECT7765
Macrophages
Cytokines
id ES_92c6810938cb2e99c3a80d7ba65ca3a2
oai_identifier_str oai:fisabio.fundanetsuite.com:p2334
network_acronym_str ES
network_name_str España
repository_id_str
spelling Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosisMoratalla ACAPARROS, ESTHERJUANOLA, ORIOLPortune KPuig-Kroger AEstrada-Capetillo LBELLOT, PABLOGomez-Hurtado IPIÑERO, PAULAGONZALEZ, JOSE MANUELSuch JSanz YFRANCES, RUBÉNCirrhosisBifidobacterium pseudocatenulatum CECT7765MacrophagesCytokinesBackground & Aims: Patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. We aimed at evaluating Bifidobacterium pseudocatenulatum CECT7765 effect on the host's macrophage function. Patients & methods: Patients with cirrhosis and ascites were included. Granulocyte-macrophage colony-stimulating factor (GM-CSF) monocyte-derived and ascitic fluid (AF) macrophages were cultured with M-CSF, lipopolysaccharide (LPS) and/or the bifidobacterial strain. Pellets and supernatants were evaluated for gene expression of M1 and M2-related genes and cytokine secretion. Cell surface expression molecules were evaluated by flow cytometry. Kupffer cells from bile duct ligated and CCl4 rats were also evaluated. Results: Experiments were run on GM-CSF blood-derived and AF macrophages from 10 patients with cirrhosis and 10 healthy donors. Different macrophage morphology was observed by optical microscopy in cells stimulated with bifidobacteria vs. LPS. M2-like expression of CD206, CD163 and CD16 was significantly increased in macrophages after stimulation with the bifidobacterial strain vs. LPS. B. pseudocatenulatum CECT7765 was able to significantly change the cytokine secretion pattern of blood-derived and AF macrophages and Kupffer cells from bile duct ligated and CCl4 cirrhotic rats compared to that induced by LPS. B. pseudocatenulatum CECT7765 was also effective in inducing a phenotype transition and a functional change from an M1- to an M2-related gene expression and cytokine secretion pattern in AF macrophages even after LPS-pretreatment. B. pseudocatenulatum CECT7765 did not reduce AF macrophage bacterial killing capacity. Conclusion: B. pseudocatenulatum CECT7765 induces a morphologic, phenotypic and functional transition towards an anti-inflammatory profile in GM-CSF monocyte-derived and AF macrophages from patients with cirrhosis that may help in controlling sustained inflammation in decompensated cirrhosis. (C) 2015 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.ELSEVIER2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://fisabio.portalinvestigacion.com/publicaciones/2334JOURNAL OF HEPATOLOGYISSN: 01688278ISSNe: 16000641reponame:r-FISABIO. Repositorio Institucional de Producción Científicainstname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)Inglésinfo:eu-repo/semantics/openAccessoai:fisabio.fundanetsuite.com:p23342026-06-11T12:45:17Z
dc.title.none.fl_str_mv Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
title Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
spellingShingle Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
Moratalla A
Cirrhosis
Bifidobacterium pseudocatenulatum CECT7765
Macrophages
Cytokines
title_short Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
title_full Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
title_fullStr Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
title_full_unstemmed Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
title_sort Bifidobacterium pseudocatenulatum CECT7765 induces an M2 anti-inflammatory transition in macrophages from patients with cirrhosis
dc.creator.none.fl_str_mv Moratalla A
CAPARROS, ESTHER
JUANOLA, ORIOL
Portune K
Puig-Kroger A
Estrada-Capetillo L
BELLOT, PABLO
Gomez-Hurtado I
PIÑERO, PAULA
GONZALEZ, JOSE MANUEL
Such J
Sanz Y
FRANCES, RUBÉN
author Moratalla A
author_facet Moratalla A
CAPARROS, ESTHER
JUANOLA, ORIOL
Portune K
Puig-Kroger A
Estrada-Capetillo L
BELLOT, PABLO
Gomez-Hurtado I
PIÑERO, PAULA
GONZALEZ, JOSE MANUEL
Such J
Sanz Y
FRANCES, RUBÉN
author_role author
author2 CAPARROS, ESTHER
JUANOLA, ORIOL
Portune K
Puig-Kroger A
Estrada-Capetillo L
BELLOT, PABLO
Gomez-Hurtado I
PIÑERO, PAULA
GONZALEZ, JOSE MANUEL
Such J
Sanz Y
FRANCES, RUBÉN
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cirrhosis
Bifidobacterium pseudocatenulatum CECT7765
Macrophages
Cytokines
topic Cirrhosis
Bifidobacterium pseudocatenulatum CECT7765
Macrophages
Cytokines
description Background & Aims: Patients with cirrhosis show recurrent access of bacterial products into the bloodstream inducing a multi-altered immunological status leading to relevant complications. We aimed at evaluating Bifidobacterium pseudocatenulatum CECT7765 effect on the host's macrophage function. Patients & methods: Patients with cirrhosis and ascites were included. Granulocyte-macrophage colony-stimulating factor (GM-CSF) monocyte-derived and ascitic fluid (AF) macrophages were cultured with M-CSF, lipopolysaccharide (LPS) and/or the bifidobacterial strain. Pellets and supernatants were evaluated for gene expression of M1 and M2-related genes and cytokine secretion. Cell surface expression molecules were evaluated by flow cytometry. Kupffer cells from bile duct ligated and CCl4 rats were also evaluated. Results: Experiments were run on GM-CSF blood-derived and AF macrophages from 10 patients with cirrhosis and 10 healthy donors. Different macrophage morphology was observed by optical microscopy in cells stimulated with bifidobacteria vs. LPS. M2-like expression of CD206, CD163 and CD16 was significantly increased in macrophages after stimulation with the bifidobacterial strain vs. LPS. B. pseudocatenulatum CECT7765 was able to significantly change the cytokine secretion pattern of blood-derived and AF macrophages and Kupffer cells from bile duct ligated and CCl4 cirrhotic rats compared to that induced by LPS. B. pseudocatenulatum CECT7765 was also effective in inducing a phenotype transition and a functional change from an M1- to an M2-related gene expression and cytokine secretion pattern in AF macrophages even after LPS-pretreatment. B. pseudocatenulatum CECT7765 did not reduce AF macrophage bacterial killing capacity. Conclusion: B. pseudocatenulatum CECT7765 induces a morphologic, phenotypic and functional transition towards an anti-inflammatory profile in GM-CSF monocyte-derived and AF macrophages from patients with cirrhosis that may help in controlling sustained inflammation in decompensated cirrhosis. (C) 2015 European Association for the Study of the Liver. Published by Elsevier B.V. All rights reserved.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://fisabio.portalinvestigacion.com/publicaciones/2334
url https://fisabio.portalinvestigacion.com/publicaciones/2334
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv ELSEVIER
publisher.none.fl_str_mv ELSEVIER
dc.source.none.fl_str_mv JOURNAL OF HEPATOLOGY
ISSN: 01688278
ISSNe: 16000641
reponame:r-FISABIO. Repositorio Institucional de Producción Científica
instname:Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
instname_str Fundación para el Fomento de la Investigación Sanitaria y Biomédica de la Comunitat Valenciana (FISABIO)
reponame_str r-FISABIO. Repositorio Institucional de Producción Científica
collection r-FISABIO. Repositorio Institucional de Producción Científica
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413497723420673
score 15,812455

Registos relacionados