Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection

Ecosystem monitoring is a fundamental tool to avert biodiversity loss, gathering valuable information that can be used to develop conservation policies, evaluating management outcomes, and guiding science-based decision-making. The Mar Menor costal lagoon (South-East of Spain) has experienced episod...

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Detalhes bibliográficos
Autores: Corral-Lou, Andrea, Ramón-Laca, Ana, Alcaraz, Lourdes, Cassidy, Robert, Taboada, Sergi, Mariani, Stefano, Mercado, Borja, Vicente, Martin, Pérez-Ruzafa, Ángel, Gallego, Ramón, Riesgo Gil, Ana
Formato: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2024
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/388358
Acesso em linha:http://hdl.handle.net/10261/388358
Access Level:acceso abierto
Palavra-chave:Ecological disturbance
Eutrophication
Metabarcoding
eDNA
Natural sampler
Marine ecology
Coastal lagoons
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network_acronym_str ES
network_name_str España
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dc.title.none.fl_str_mv Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
title Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
spellingShingle Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
Corral-Lou, Andrea
Ecological disturbance
Eutrophication
Metabarcoding
eDNA
Natural sampler
Marine ecology
Coastal lagoons
title_short Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
title_full Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
title_fullStr Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
title_full_unstemmed Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
title_sort Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collection
dc.creator.none.fl_str_mv Corral-Lou, Andrea
Ramón-Laca, Ana
Alcaraz, Lourdes
Cassidy, Robert
Taboada, Sergi
Mariani, Stefano
Mercado, Borja
Vicente, Martin
Pérez-Ruzafa, Ángel
Gallego, Ramón
Riesgo Gil, Ana
author Corral-Lou, Andrea
author_facet Corral-Lou, Andrea
Ramón-Laca, Ana
Alcaraz, Lourdes
Cassidy, Robert
Taboada, Sergi
Mariani, Stefano
Mercado, Borja
Vicente, Martin
Pérez-Ruzafa, Ángel
Gallego, Ramón
Riesgo Gil, Ana
author_role author
author2 Ramón-Laca, Ana
Alcaraz, Lourdes
Cassidy, Robert
Taboada, Sergi
Mariani, Stefano
Mercado, Borja
Vicente, Martin
Pérez-Ruzafa, Ángel
Gallego, Ramón
Riesgo Gil, Ana
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Gobierno de la Región de Murcia
European Commission
Fundación Biodiversidad
Natural Environment Research Council (UK)
Consejo Superior de Investigaciones Científicas (España)
Ministerio de Ciencia e Innovación (España)
Corral-Lou, Andrea [0000-0003-0895-1305]
Ramón-Laca, Ana [0000-0002-9204-6932]
Taboada, Sergio [0000-0003-1669-1152]
Riesgo Gil, Ana [0000-0002-7993-1523]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Ecological disturbance
Eutrophication
Metabarcoding
eDNA
Natural sampler
Marine ecology
Coastal lagoons
topic Ecological disturbance
Eutrophication
Metabarcoding
eDNA
Natural sampler
Marine ecology
Coastal lagoons
description Ecosystem monitoring is a fundamental tool to avert biodiversity loss, gathering valuable information that can be used to develop conservation policies, evaluating management outcomes, and guiding science-based decision-making. The Mar Menor costal lagoon (South-East of Spain) has experienced episodes of eutrophication due to intensive agriculture and other human activities, causing mass mortalities of marine fauna. In this scenario, biodiversity monitoring is crucial to evaluate the status of fauna and flora and take appropriate measures. Here, our main objective was to assess faunal composition and its spatial and temporal structure associated with the pillars used to support the built recreational well-being facilities along the Mar Menor. We capitalised on the many sea sponges that settle on these structures by collecting tissue samples for subsequent extraction of filtered environmental DNA (i.e. ‘natural sampler DNA’, nsDNA) in northern and southern areas of the lagoon, both in spring and summer. We metabarcoded all samples at the cytochrome oxidase subunit I gene (COI), and reliably identified 76 taxa belonging to nine different phyla, with annelids, poriferans, molluscs and cnidarians being the richest groups. We detected emblematic species of threatened status, such as pipefishes (genus Syngnathus) and the piddock clam, Pholas dactylus, and others known to become invasive, such as the ascidians Styela canopus and Botrylloides niger, the barnacle Amphibalanus amphitrite, and the polychaetes Branchiomma bairdi and Branchiomma boholense. The use of common and widely distributed sponges as natural eDNA samplers allowed us to characterise both spatial and temporal diversity, further emphasizing the importance of this low-cost approach to monitoring biological communities in shallow coastal ecosystems.
publishDate 2024
dc.date.none.fl_str_mv 2024
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/388358
url http://hdl.handle.net/10261/388358
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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dc.publisher.none.fl_str_mv BioRxiv
publisher.none.fl_str_mv BioRxiv
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
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reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Spatial and seasonal biodiversity variation in a large Mediterranean lagoon using environmental DNA metabarcoding through sponge tissue collectionCorral-Lou, AndreaRamón-Laca, AnaAlcaraz, LourdesCassidy, RobertTaboada, SergiMariani, StefanoMercado, BorjaVicente, MartinPérez-Ruzafa, ÁngelGallego, RamónRiesgo Gil, AnaEcological disturbanceEutrophicationMetabarcodingeDNANatural samplerMarine ecologyCoastal lagoonsEcosystem monitoring is a fundamental tool to avert biodiversity loss, gathering valuable information that can be used to develop conservation policies, evaluating management outcomes, and guiding science-based decision-making. The Mar Menor costal lagoon (South-East of Spain) has experienced episodes of eutrophication due to intensive agriculture and other human activities, causing mass mortalities of marine fauna. In this scenario, biodiversity monitoring is crucial to evaluate the status of fauna and flora and take appropriate measures. Here, our main objective was to assess faunal composition and its spatial and temporal structure associated with the pillars used to support the built recreational well-being facilities along the Mar Menor. We capitalised on the many sea sponges that settle on these structures by collecting tissue samples for subsequent extraction of filtered environmental DNA (i.e. ‘natural sampler DNA’, nsDNA) in northern and southern areas of the lagoon, both in spring and summer. We metabarcoded all samples at the cytochrome oxidase subunit I gene (COI), and reliably identified 76 taxa belonging to nine different phyla, with annelids, poriferans, molluscs and cnidarians being the richest groups. We detected emblematic species of threatened status, such as pipefishes (genus Syngnathus) and the piddock clam, Pholas dactylus, and others known to become invasive, such as the ascidians Styela canopus and Botrylloides niger, the barnacle Amphibalanus amphitrite, and the polychaetes Branchiomma bairdi and Branchiomma boholense. The use of common and widely distributed sponges as natural eDNA samplers allowed us to characterise both spatial and temporal diversity, further emphasizing the importance of this low-cost approach to monitoring biological communities in shallow coastal ecosystems.APR acknowledges funding from “Monitoring and predictive analysis of the ecological state evolution of the Mar Menor lagoon ecosystem and prevention of impacts 2020-2022 and 2023” financed by the General Directorate of the Mar Menor of the Region of Murcia that also have contributed to the contracts of BMC and MVR. AR acknowledges funding from the SponBIODIV project (granted to AR and ST), a 2021-2022 BiodivProtect joint call for research proposals, under the Biodiversa+ Partnership co-funded by the European Commission, and with the funding organisations ‘Fundación Biodiversidad’ and FORMAS, the grant NE/T007028/1 from the UK Natural Environment Research Council (to SM and AR), an intramural grant from CSIC (PIE-202030E006) to AR, and three grants from the Spanish Ministry of Science and Innovation (RYC2018-024247-I, PID2019-105769GB-I00, and CNS2023-144571) in the framework of MCIN/AEI/10.13039/50110001103 and EI “FSE invierte en tu futuro”, to AR. ST received funding from the grants PID2020-117115GA-100 and by CNS2023-144572 funded by MCIN/AEI/10.13039/501100011033 and by the Ramón y Cajal grant RYC2021-03152-I, funded by the MCIN/AEI/10.13039/501100011033 and the European Union «NextGenerationEU/PRTR».NoBioRxivGobierno de la Región de MurciaEuropean CommissionFundación BiodiversidadNatural Environment Research Council (UK)Consejo Superior de Investigaciones Científicas (España)Ministerio de Ciencia e Innovación (España)Corral-Lou, Andrea [0000-0003-0895-1305]Ramón-Laca, Ana [0000-0002-9204-6932]Taboada, Sergio [0000-0003-1669-1152]Riesgo Gil, Ana [0000-0002-7993-1523]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/388358reponame:DIGITAL.CSIC. 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