Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation

Milk oligosaccharides (OSs) are bioactive components known to influence neonatal development. These compounds have specific physiological functions acting as prebiotics, immune system modulators, and enhancing intestine and brain development. Objectives: The pig is a commonly used model for studying...

ver descrição completa

Detalhes bibliográficos
Autores: Mudd, Austin T., Salcedo, Jaime, Alexander, Lindsey S., Johnson, Stacey K., Getty, Caitlyn M., Chichlowski, Maciej, Berg, Brian M., Barile, Daniela, Dilger, Ryan N.
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2016
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/415252
Acesso em linha:http://hdl.handle.net/10261/415252
https://api.elsevier.com/content/abstract/scopus_id/85035349488
Access Level:acceso abierto
Palavra-chave:Milk
Oligosaccharides
Porcine
Sialic acid
id ES_8faf0ca95ec319245e7f60983bc93bfa
oai_identifier_str oai:digital.csic.es:10261/415252
network_acronym_str ES
network_name_str España
repository_id_str
spelling Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout LactationMudd, Austin T.Salcedo, JaimeAlexander, Lindsey S.Johnson, Stacey K.Getty, Caitlyn M.Chichlowski, MaciejBerg, Brian M.Barile, DanielaDilger, Ryan N.MilkOligosaccharidesPorcineSialic acidMilk oligosaccharides (OSs) are bioactive components known to influence neonatal development. These compounds have specific physiological functions acting as prebiotics, immune system modulators, and enhancing intestine and brain development. Objectives: The pig is a commonly used model for studying human nutrition, and there is interest in quantifying OS composition of porcine milk across lactation compared with human milk. In this study, we hypothesized that OS and sialic acid (SA) composition of porcine milk would be influenced by stage of lactation. Methods: Up to 250 mL of milk were collected from seven sows at each of three time points: day 0 (colostrum), days 7–9 (mature), and days 17–19 (weaning). Colostrum was collected within 6 h of farrowing and 3-day intervals were used for mature and weaning milk to ensure representative sampling. Milk samples were analyzed for OS profiles by Nano-LC Chip–QTOF MS, OS concentrations via HPAEC-PAD, and SA (total and free) was assessed by enzymatic reaction fluorescence detection. Results: Sixty unique OSs were identified in porcine milk. Neutral OSs were the most abundant at each lactation stage (69–81%), followed by acidic-sialylated OSs (16–29%) and neutral-fucosylated OSs (2–4%). As lactation progressed, acidic OSs decreased (P = 0.003), whereas neutral-fucosylated (P < 0.001) and neutral OSs (P = 0.003) increased throughout lactation. Six OSs were present in all samples analyzed across lactation [lacto-N-difucohexaose I (LNDFH-I), 2′-fucosyllactose (2′-FL), lacto-N-fucopentaose I (LNFP-I), lacto-N-neohexaose (LNnH), α1-3,β-4-d-galactotriose (3-Hex), 3′-sialyllactose (3′-SL)], while LDFT was present only in colostrum samples. Analysis of individual OS concentrations indicated differences (P = 0.023) between days 0 and 7. Conversely, between days 7 and 18, OS concentrations remained stable with only LNnH (P < 0.001) and LNDFH-I (P = 0.002) decreasing over this period. Analysis of free SA indicated a decrease (P < 0.001) as lactation progressed, while bound (P < 0.001) and total (P < 0.001) SA increased across lactation. Conclusion: Concentrations of OS differ between colostrum and mature milk in the pig, and SA concentrations shift from free to bound forms as lactation progresses. Our results suggest that although porcine milk OS concentration and the number of structures is lower than human milk, the OS profile appears to be closer to human milk rather than to bovine milk, based on previously published profiles.This work was partially supported by the United States Department of Agriculture National Institute of Food and Agriculture, Hatch project 1009051.This project was also partially funded by Mead Johnson Pediatric Nutrition Institute.Peer reviewedFrontiers MediaNational Institute of Food and Agriculture (US)202620262016info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/415252https://api.elsevier.com/content/abstract/scopus_id/85035349488reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésFrontiers in nutritionhttps://doi.org/10.3389/fnut.2016.00039Noinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4152522026-05-22T06:33:51Z
dc.title.none.fl_str_mv Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
title Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
spellingShingle Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
Mudd, Austin T.
Milk
Oligosaccharides
Porcine
Sialic acid
title_short Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
title_full Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
title_fullStr Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
title_full_unstemmed Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
title_sort Porcine Milk Oligosaccharides and Sialic Acid Concentrations Vary Throughout Lactation
dc.creator.none.fl_str_mv Mudd, Austin T.
Salcedo, Jaime
Alexander, Lindsey S.
Johnson, Stacey K.
Getty, Caitlyn M.
Chichlowski, Maciej
Berg, Brian M.
Barile, Daniela
Dilger, Ryan N.
author Mudd, Austin T.
author_facet Mudd, Austin T.
Salcedo, Jaime
Alexander, Lindsey S.
Johnson, Stacey K.
Getty, Caitlyn M.
Chichlowski, Maciej
Berg, Brian M.
Barile, Daniela
Dilger, Ryan N.
author_role author
author2 Salcedo, Jaime
Alexander, Lindsey S.
Johnson, Stacey K.
Getty, Caitlyn M.
Chichlowski, Maciej
Berg, Brian M.
Barile, Daniela
Dilger, Ryan N.
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Institute of Food and Agriculture (US)
dc.subject.none.fl_str_mv Milk
Oligosaccharides
Porcine
Sialic acid
topic Milk
Oligosaccharides
Porcine
Sialic acid
description Milk oligosaccharides (OSs) are bioactive components known to influence neonatal development. These compounds have specific physiological functions acting as prebiotics, immune system modulators, and enhancing intestine and brain development. Objectives: The pig is a commonly used model for studying human nutrition, and there is interest in quantifying OS composition of porcine milk across lactation compared with human milk. In this study, we hypothesized that OS and sialic acid (SA) composition of porcine milk would be influenced by stage of lactation. Methods: Up to 250 mL of milk were collected from seven sows at each of three time points: day 0 (colostrum), days 7–9 (mature), and days 17–19 (weaning). Colostrum was collected within 6 h of farrowing and 3-day intervals were used for mature and weaning milk to ensure representative sampling. Milk samples were analyzed for OS profiles by Nano-LC Chip–QTOF MS, OS concentrations via HPAEC-PAD, and SA (total and free) was assessed by enzymatic reaction fluorescence detection. Results: Sixty unique OSs were identified in porcine milk. Neutral OSs were the most abundant at each lactation stage (69–81%), followed by acidic-sialylated OSs (16–29%) and neutral-fucosylated OSs (2–4%). As lactation progressed, acidic OSs decreased (P = 0.003), whereas neutral-fucosylated (P < 0.001) and neutral OSs (P = 0.003) increased throughout lactation. Six OSs were present in all samples analyzed across lactation [lacto-N-difucohexaose I (LNDFH-I), 2′-fucosyllactose (2′-FL), lacto-N-fucopentaose I (LNFP-I), lacto-N-neohexaose (LNnH), α1-3,β-4-d-galactotriose (3-Hex), 3′-sialyllactose (3′-SL)], while LDFT was present only in colostrum samples. Analysis of individual OS concentrations indicated differences (P = 0.023) between days 0 and 7. Conversely, between days 7 and 18, OS concentrations remained stable with only LNnH (P < 0.001) and LNDFH-I (P = 0.002) decreasing over this period. Analysis of free SA indicated a decrease (P < 0.001) as lactation progressed, while bound (P < 0.001) and total (P < 0.001) SA increased across lactation. Conclusion: Concentrations of OS differ between colostrum and mature milk in the pig, and SA concentrations shift from free to bound forms as lactation progresses. Our results suggest that although porcine milk OS concentration and the number of structures is lower than human milk, the OS profile appears to be closer to human milk rather than to bovine milk, based on previously published profiles.
publishDate 2016
dc.date.none.fl_str_mv 2016
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/415252
https://api.elsevier.com/content/abstract/scopus_id/85035349488
url http://hdl.handle.net/10261/415252
https://api.elsevier.com/content/abstract/scopus_id/85035349488
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Frontiers in nutrition
https://doi.org/10.3389/fnut.2016.00039
No
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Frontiers Media
publisher.none.fl_str_mv Frontiers Media
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869413232078225409
score 15,228081

Registos relacionados