miRNA profiling during antigendependent T cell activation: A role for miR-132-3p

microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common t...

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Detalhes bibliográficos
Autores: Gutiérrez-Vázquez, Cristina, Rodríguez Galán, Ana, Fernández-Alfara, Marcos, MITTELBRUM HERRERO, MARIA, Sánchez Cabo, Fátima, Martínez-Herrera, Dannys Jorge, Ramírez-Huesca, Marta, Pascual-Montano, Alberto, Sánchez Madrid, Francisco
Tipo de documento: artigo
Data de publicação:2017
País:España
Recursos:Universidad Autónoma de Madrid
Repositório:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:inglês
OAI Identifier:oai:repositorio.uam.es:10486/680259
Acesso em linha:http://hdl.handle.net/10486/680259
https://dx.doi.org/10.1038/s41598-017-03689-7
Access Level:Acceso aberto
Palavra-chave:microRNAs (miRNAs)
T cell activation
Pik3r1 gene
MiR-132-3p
Medicina
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spelling miRNA profiling during antigendependent T cell activation: A role for miR-132-3pGutiérrez-Vázquez, CristinaRodríguez Galán, AnaFernández-Alfara, MarcosMITTELBRUM HERRERO, MARIASánchez Cabo, FátimaMartínez-Herrera, Dannys JorgeRamírez-Huesca, MartaPascual-Montano, AlbertoSánchez Madrid, FranciscomicroRNAs (miRNAs)T cell activationPik3r1 geneMiR-132-3pMedicinamicroRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cellsThis work was supported by grants SAF2014-55579R from Ministerio de Economía y Competitividad-Spain, ERC-2011-AdG 294340-GENTRIS, CIBER CARDIOVASCULAR (FEDER and Instituto de Salud Carlos III), PIE-13-00041 and INDISNET S2011-BMD-2332 (F. S. M.). The Centro Nacional de Investigaciones Cardiovasculares (CNIC, Spain) is supported by the Ministerio de Economía y Competitividad-Spain and the Pro-CNIC FoundationNature Publishing GroupDepartamento de MedicinaFacultad de MedicinaInstituto de Investigación del Hospital de La Princesa (IP)20172017-06-14research articlehttp://purl.org/coar/resource_type/c_2df8fbb1VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10486/680259https://dx.doi.org/10.1038/s41598-017-03689-7reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6802592026-06-23T12:46:27Z
dc.title.none.fl_str_mv miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
title miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
spellingShingle miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
Gutiérrez-Vázquez, Cristina
microRNAs (miRNAs)
T cell activation
Pik3r1 gene
MiR-132-3p
Medicina
title_short miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
title_full miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
title_fullStr miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
title_full_unstemmed miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
title_sort miRNA profiling during antigendependent T cell activation: A role for miR-132-3p
dc.creator.none.fl_str_mv Gutiérrez-Vázquez, Cristina
Rodríguez Galán, Ana
Fernández-Alfara, Marcos
MITTELBRUM HERRERO, MARIA
Sánchez Cabo, Fátima
Martínez-Herrera, Dannys Jorge
Ramírez-Huesca, Marta
Pascual-Montano, Alberto
Sánchez Madrid, Francisco
author Gutiérrez-Vázquez, Cristina
author_facet Gutiérrez-Vázquez, Cristina
Rodríguez Galán, Ana
Fernández-Alfara, Marcos
MITTELBRUM HERRERO, MARIA
Sánchez Cabo, Fátima
Martínez-Herrera, Dannys Jorge
Ramírez-Huesca, Marta
Pascual-Montano, Alberto
Sánchez Madrid, Francisco
author_role author
author2 Rodríguez Galán, Ana
Fernández-Alfara, Marcos
MITTELBRUM HERRERO, MARIA
Sánchez Cabo, Fátima
Martínez-Herrera, Dannys Jorge
Ramírez-Huesca, Marta
Pascual-Montano, Alberto
Sánchez Madrid, Francisco
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Departamento de Medicina
Facultad de Medicina
Instituto de Investigación del Hospital de La Princesa (IP)
dc.subject.none.fl_str_mv microRNAs (miRNAs)
T cell activation
Pik3r1 gene
MiR-132-3p
Medicina
topic microRNAs (miRNAs)
T cell activation
Pik3r1 gene
MiR-132-3p
Medicina
description microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3′UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells
publishDate 2017
dc.date.none.fl_str_mv 2017
2017-06-14
dc.type.none.fl_str_mv research article
http://purl.org/coar/resource_type/c_2df8fbb1
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/680259
https://dx.doi.org/10.1038/s41598-017-03689-7
url http://hdl.handle.net/10486/680259
https://dx.doi.org/10.1038/s41598-017-03689-7
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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