PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation

8 p.-5 fig.

Detalles Bibliográficos
Autores: Ruiz-Albor, Antonio, Chaves-Arquero, Belén, Martín-Barros, Inés, Guerra-Castellano, Alejandra, González-Magaña, Amaia, Ibáñez de Opakua, Alain, Merino, Nekane, Ferreras-Gutiérrez, Mariola, Berra, Edurne, Díaz-Moreno, Irene, Blanco, Francisco J.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/360965
Acceso en línea:http://hdl.handle.net/10261/360965
Access Level:acceso abierto
Palabra clave:PCNA
Molecular recognition
DNA replication
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spelling PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylationRuiz-Albor, AntonioChaves-Arquero, BelénMartín-Barros, InésGuerra-Castellano, AlejandraGonzález-Magaña, AmaiaIbáñez de Opakua, AlainMerino, NekaneFerreras-Gutiérrez, MariolaBerra, EdurneDíaz-Moreno, IreneBlanco, Francisco J.PCNAMolecular recognitionDNA replication8 p.-5 fig.The coordination of enzymes and regulatory proteins for eukaryotic DNA replication and repair is largely achieved by Proliferating Cell Nuclear Antigen (PCNA), a toroidal homotrimeric protein that embraces the DNA duplex. Many proteins bind PCNA through a conserved sequence known as the PCNA interacting protein motif (PIP). PCNA is further regulated by different post-translational modifications. Phosphorylation at residue Y211 facilitates unlocking stalled replication forks to bypass DNA damage repair processes but increasing nucleotide misincorporation. We explore here how phosphorylation at Y211 affects PCNA recognition of the canonical PIP sequences of the regulatory proteins p21 and p15, which bind with nM and μM affinity, respectively. For that purpose, we have prepared PCNA with p-carboxymethyl-L-phenylalanine (pCMF, a mimetic of phosphorylated tyrosine) at position 211. We have also characterized PCNA binding to the non-canonical PIP sequence of the catalytic subunit of DNA polymerase δ (p125), and to the canonical PIP sequence of the enzyme ubiquitin specific peptidase 29 (USP29) which deubiquitinates PCNA. Our results show that Tyr211 phosphorylation has little effect on the molecular recognition of p21 and p15, and that the PIP sequences of p125 and USP29 bind to the same site on PCNA as other PIP sequences, but with very low affinity.This work was supported by the Agencia Estatal de Investigación (AEI), the Spanish Ministry of Science and Innovation (MCIN) and “ERDF A way of making Europe” (MCIN/AEI/10.13039/501100011033, grant numbers PID2020-113225GB-I00 to FJB, PID2021-126663NB-I00 to ID-M, and predoctoral contracts PRE2021-099992 to ARA and PRE2018-085788 to MFG). Work at ID-M laboratory is also supported by The European Regional Development Fund (FEDER), the Andalusian Government (BIO-198), and the Ramón Areces Foundation. We also acknowledge the support from the network PIE-202120E047-Conexión-Life, CSIC.Peer reviewedElsevierAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionJunta de AndalucíaFundación Ramón ArecesConsejo Superior de Investigaciones Científicas (España)Ruiz-Albor, Antonio [0000-0001-8931-8856]Chaves-Arquero, Belén [0000-0002-2761-2336]Guerra-Castellano, Alejandra [0000-0001-5823-1678]González-Magaña, Amaia [0000-0002-6007-8390]Ibáñez de Opakua, Alain [0000-0003-1700-9354]Merino, Nekane [0000-0003-0721-4813]Ferreras-Gutiérrez, Mariola [0000-0003-4421-3158]Berra, Edurne [0000-0002-3820-5744]Díaz-Moreno, Irene [0000-0002-5318-7644]Blanco, Francisco J. [0000-0003-2545-4319]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/360965reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113225GB-I00info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126663NB-I00https://doi.org/10.1016/j.ijbiomac.2024.133187Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3609652026-05-22T06:33:51Z
dc.title.none.fl_str_mv PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
title PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
spellingShingle PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
Ruiz-Albor, Antonio
PCNA
Molecular recognition
DNA replication
title_short PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
title_full PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
title_fullStr PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
title_full_unstemmed PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
title_sort PCNA molecular recognition of different PIP motifs: Role of Tyr211 phosphorylation
dc.creator.none.fl_str_mv Ruiz-Albor, Antonio
Chaves-Arquero, Belén
Martín-Barros, Inés
Guerra-Castellano, Alejandra
González-Magaña, Amaia
Ibáñez de Opakua, Alain
Merino, Nekane
Ferreras-Gutiérrez, Mariola
Berra, Edurne
Díaz-Moreno, Irene
Blanco, Francisco J.
author Ruiz-Albor, Antonio
author_facet Ruiz-Albor, Antonio
Chaves-Arquero, Belén
Martín-Barros, Inés
Guerra-Castellano, Alejandra
González-Magaña, Amaia
Ibáñez de Opakua, Alain
Merino, Nekane
Ferreras-Gutiérrez, Mariola
Berra, Edurne
Díaz-Moreno, Irene
Blanco, Francisco J.
author_role author
author2 Chaves-Arquero, Belén
Martín-Barros, Inés
Guerra-Castellano, Alejandra
González-Magaña, Amaia
Ibáñez de Opakua, Alain
Merino, Nekane
Ferreras-Gutiérrez, Mariola
Berra, Edurne
Díaz-Moreno, Irene
Blanco, Francisco J.
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
European Commission
Junta de Andalucía
Fundación Ramón Areces
Consejo Superior de Investigaciones Científicas (España)
Ruiz-Albor, Antonio [0000-0001-8931-8856]
Chaves-Arquero, Belén [0000-0002-2761-2336]
Guerra-Castellano, Alejandra [0000-0001-5823-1678]
González-Magaña, Amaia [0000-0002-6007-8390]
Ibáñez de Opakua, Alain [0000-0003-1700-9354]
Merino, Nekane [0000-0003-0721-4813]
Ferreras-Gutiérrez, Mariola [0000-0003-4421-3158]
Berra, Edurne [0000-0002-3820-5744]
Díaz-Moreno, Irene [0000-0002-5318-7644]
Blanco, Francisco J. [0000-0003-2545-4319]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv PCNA
Molecular recognition
DNA replication
topic PCNA
Molecular recognition
DNA replication
description 8 p.-5 fig.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/360965
url http://hdl.handle.net/10261/360965
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2020-113225GB-I00
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2021-126663NB-I00
https://doi.org/10.1016/j.ijbiomac.2024.133187
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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