Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation

Imprinted genes control several cellular and metabolic processes in embryonic and adult tissues. In particular, paternally expressed gene-3 (Peg3) is active in the adult stem cell population and during muscle and neuronal lineage development. Here we have investigated the role of Peg3 in mouse embry...

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Autores: Theka, Ilda, 1984-, Sottile, Francesco, 1988-, Aulicino, Francesco, 1987-, García, Álvaro Castells, Cosma, Maria Pia
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2017
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/35159
Acesso em linha:http://hdl.handle.net/10230/35159
http://dx.doi.org/10.1038/s41598-017-10016-7
Access Level:acceso abierto
Palavra-chave:Embryonic stem cells
Induced pluripotent stem cells
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dc.title.none.fl_str_mv Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
title Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
spellingShingle Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
Theka, Ilda, 1984-
Embryonic stem cells
Induced pluripotent stem cells
title_short Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
title_full Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
title_fullStr Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
title_full_unstemmed Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
title_sort Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbation
dc.creator.none.fl_str_mv Theka, Ilda, 1984-
Sottile, Francesco, 1988-
Aulicino, Francesco, 1987-
García, Álvaro Castells
Cosma, Maria Pia
author Theka, Ilda, 1984-
author_facet Theka, Ilda, 1984-
Sottile, Francesco, 1988-
Aulicino, Francesco, 1987-
García, Álvaro Castells
Cosma, Maria Pia
author_role author
author2 Sottile, Francesco, 1988-
Aulicino, Francesco, 1987-
García, Álvaro Castells
Cosma, Maria Pia
author2_role author
author
author
author
dc.subject.none.fl_str_mv Embryonic stem cells
Induced pluripotent stem cells
topic Embryonic stem cells
Induced pluripotent stem cells
description Imprinted genes control several cellular and metabolic processes in embryonic and adult tissues. In particular, paternally expressed gene-3 (Peg3) is active in the adult stem cell population and during muscle and neuronal lineage development. Here we have investigated the role of Peg3 in mouse embryonic stem cells (ESCs) and during the process of somatic cell reprogramming towards pluripotency. Our data show that Peg3 knockdown increases expression of pluripotency genes in ESCs and enhances reprogramming efficiency of both mouse embryonic fibroblasts and neural stem cells. Interestingly, we observed that altered activity of Peg3 correlates with major perturbations of mitochondrial gene expression and mitochondrial function, which drive metabolic changes during somatic cell reprogramming. Overall, our study shows that Peg3 is a regulator of pluripotent stem cells and somatic cell reprogramming.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/35159
http://dx.doi.org/10.1038/s41598-017-10016-7
url http://hdl.handle.net/10230/35159
http://dx.doi.org/10.1038/s41598-017-10016-7
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Scientific Reports. 2017 Aug 29;7(1):9705
info:eu-repo/grantAgreement/EC/H2020/686637
info:eu-repo/grantAgreement/ES/3PN/SAF2011-28580
info:eu-repo/grantAgreement/ES/1PE/BFU2014-54717-P
info:eu-repo/grantAgreement/ES/1PE/BFU2015-71984-ERC
info:eu-repo/grantAgreement/EC/FP7/290123
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
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dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
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spelling Reduced expression of Paternally Expressed Gene-3 enhances somatic cell reprogramming through mitochondrial activity perturbationTheka, Ilda, 1984-Sottile, Francesco, 1988-Aulicino, Francesco, 1987-García, Álvaro CastellsCosma, Maria PiaEmbryonic stem cellsInduced pluripotent stem cellsImprinted genes control several cellular and metabolic processes in embryonic and adult tissues. In particular, paternally expressed gene-3 (Peg3) is active in the adult stem cell population and during muscle and neuronal lineage development. Here we have investigated the role of Peg3 in mouse embryonic stem cells (ESCs) and during the process of somatic cell reprogramming towards pluripotency. Our data show that Peg3 knockdown increases expression of pluripotency genes in ESCs and enhances reprogramming efficiency of both mouse embryonic fibroblasts and neural stem cells. Interestingly, we observed that altered activity of Peg3 correlates with major perturbations of mitochondrial gene expression and mitochondrial function, which drive metabolic changes during somatic cell reprogramming. Overall, our study shows that Peg3 is a regulator of pluripotent stem cells and somatic cell reprogramming.We thank Marie Victoire Neguembor, Martina Pesaresi and Sergi Angel Bonilla Pons for suggestions on the manuscript and Umberto Di Vicino for technical support. We thank Angelique di Domenico and the group of Antonella Consiglio for providing us the OPA1, DRP1 and VDAC1 antibodies. We thank Mari Carmen Ortells and the group of Bill Keyes for providing us the OXPHOS antibody. The pInducer10-mir-RUP-PheS plasmid was a gift from Stephen Elledge (Addgene plasmid # 44011). We are grateful for support from Ministerio de Economia y Competitividad and FEDER funds (SAF2011-28580, BFU2014-54717-P, and BFU2015-71984-ERC to M.P.C.), Secretaria d'Universitats i Recerca del Departament d'Economia i Coneixement de la Generalitat de Catalunya (2014 SGR1137 to M.P.C.), the European Union's Horizon 2020 research and innovation programme under grant agreement CellViewer No 686637 (to M.P.C.), Spanish Ministry of Economy and Competitiveness, 'Centro de Excelencia Severo Ochoa 2013-2017 and the CERCA Programme/Generalitat de Catalunya (to M.P.C.), People Programme Marie Curie Actions of the European Union's Seventh Framework Programme (FP7/2007-2013/, n° 290123 to I.T.), La Caixa international PhD fellowship (to F.S.), Ministerio de Ciencia e Innovació FPI (to F.A.) and Ministerio de Ciencia e Innovación FPI-Severo Ochoa (to A.C.G).Nature Publishing Group201820182017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/35159http://dx.doi.org/10.1038/s41598-017-10016-7reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésScientific Reports. 2017 Aug 29;7(1):9705info:eu-repo/grantAgreement/EC/H2020/686637info:eu-repo/grantAgreement/ES/3PN/SAF2011-28580info:eu-repo/grantAgreement/ES/1PE/BFU2014-54717-Pinfo:eu-repo/grantAgreement/ES/1PE/BFU2015-71984-ERCinfo:eu-repo/grantAgreement/EC/FP7/290123© The Author(s) 2017. Open Access. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/351592026-05-29T05:05:01Z
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