Structure and function of Full-length Tau

Tau protein, encoded by the MAPT gene, is a microtubule-associated protein involved in the regulation of microtubule stability in neurons, contributing to cell shape maintenance and intracellular transport, among other functions. Tau is not found as a unique isoform; instead, different Tau isoforms...

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Detalles Bibliográficos
Autores: Vallés-Saiz, Laura, Domene-Serrano, Indalo, Picher, Angel J., Pérez, Mar, García-Escudero, Vega, Hernández, Félix, Avila, Jesús
Tipo de recurso: artículo
Fecha de publicación:2025
País:España
Institución:Universidad Francisco de Vitoria
Repositorio:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
Idioma:inglés
OAI Identifier:oai:ddfv.ufv.es:10641/7575
Acceso en línea:https://hdl.handle.net/10641/7575
Access Level:acceso abierto
Palabra clave:General
Journal Article
Yes
yes
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spelling Structure and function of Full-length TauVallés-Saiz, LauraDomene-Serrano, IndaloPicher, Angel J.Pérez, MarGarcía-Escudero, VegaHernández, FélixAvila, JesúsGeneralJournal ArticleYesyesTau protein, encoded by the MAPT gene, is a microtubule-associated protein involved in the regulation of microtubule stability in neurons, contributing to cell shape maintenance and intracellular transport, among other functions. Tau is not found as a unique isoform; instead, different Tau isoforms of varying sizes are present in the brain, but a Full-length Tau isoform (Full Tau) containing all 16 exons has never been previously identified. This study has explored the structure and function of the Full Tau isoform, which includes all exons of the MAPT gene. To achieve this, we expressed the Full Tau isoform in bacteria, alongside the Tau 4R2N isoform as a control, and tested its microtubule-binding capacity, self-aggregation propensity, and effects on cultured cells regarding cell proliferation and cell death. Our results indicated several differences between the Full Tau and Tau 4R2N isoforms, suggesting distinct roles in cellular dynamics. To explain these differences, we suggest the role of exon 8, which is present in the Full Tau isoform but absent in Tau 4R2N.Facultad de Ciencias Experimentales20252025-10-0120252025-10-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10641/7575reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoriainstname:Universidad Francisco de VitoriaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2http://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddfv.ufv.es:10641/75752026-06-11T12:44:57Z
dc.title.none.fl_str_mv Structure and function of Full-length Tau
title Structure and function of Full-length Tau
spellingShingle Structure and function of Full-length Tau
Vallés-Saiz, Laura
General
Journal Article
Yes
yes
title_short Structure and function of Full-length Tau
title_full Structure and function of Full-length Tau
title_fullStr Structure and function of Full-length Tau
title_full_unstemmed Structure and function of Full-length Tau
title_sort Structure and function of Full-length Tau
dc.creator.none.fl_str_mv Vallés-Saiz, Laura
Domene-Serrano, Indalo
Picher, Angel J.
Pérez, Mar
García-Escudero, Vega
Hernández, Félix
Avila, Jesús
author Vallés-Saiz, Laura
author_facet Vallés-Saiz, Laura
Domene-Serrano, Indalo
Picher, Angel J.
Pérez, Mar
García-Escudero, Vega
Hernández, Félix
Avila, Jesús
author_role author
author2 Domene-Serrano, Indalo
Picher, Angel J.
Pérez, Mar
García-Escudero, Vega
Hernández, Félix
Avila, Jesús
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Facultad de Ciencias Experimentales
dc.subject.none.fl_str_mv General
Journal Article
Yes
yes
topic General
Journal Article
Yes
yes
description Tau protein, encoded by the MAPT gene, is a microtubule-associated protein involved in the regulation of microtubule stability in neurons, contributing to cell shape maintenance and intracellular transport, among other functions. Tau is not found as a unique isoform; instead, different Tau isoforms of varying sizes are present in the brain, but a Full-length Tau isoform (Full Tau) containing all 16 exons has never been previously identified. This study has explored the structure and function of the Full Tau isoform, which includes all exons of the MAPT gene. To achieve this, we expressed the Full Tau isoform in bacteria, alongside the Tau 4R2N isoform as a control, and tested its microtubule-binding capacity, self-aggregation propensity, and effects on cultured cells regarding cell proliferation and cell death. Our results indicated several differences between the Full Tau and Tau 4R2N isoforms, suggesting distinct roles in cellular dynamics. To explain these differences, we suggest the role of exon 8, which is present in the Full Tau isoform but absent in Tau 4R2N.
publishDate 2025
dc.date.none.fl_str_mv 2025
2025-10-01
2025
2025-10-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10641/7575
url https://hdl.handle.net/10641/7575
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2

http://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
instname:Universidad Francisco de Vitoria
instname_str Universidad Francisco de Vitoria
reponame_str DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
collection DDFV. Repositorio Institucional de la Universidad Francisco de Vitoria
repository.name.fl_str_mv
repository.mail.fl_str_mv
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