Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea

The Lluta Valley in Northern Chile is an important agricultural area affected by both salinity and boron (B) toxicity. <i>Zea mays</i> L. amylacea, an ecotype arisen because of the seed selection practiced in this valley, shows a high tolerance to salt and B levels. In the present study...

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Autores: Fuertes Mendizábal, Teresa, Bastías, Elizabeth Irica, González Murua, María del Carmen Begoña, González Moro, María Begoña
Tipo de recurso: artículo
Fecha de publicación:2020
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/42771
Acceso en línea:http://hdl.handle.net/10810/42771
Access Level:acceso abierto
Palabra clave:amino acids
glutamine synthetase
nitrate reductase
proline
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spelling Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. AmylaceaFuertes Mendizábal, TeresaBastías, Elizabeth IricaGonzález Murua, María del Carmen BegoñaGonzález Moro, María Begoñaamino acidsglutamine synthetasenitrate reductaseprolineThe Lluta Valley in Northern Chile is an important agricultural area affected by both salinity and boron (B) toxicity. <i>Zea mays</i> L. amylacea, an ecotype arisen because of the seed selection practiced in this valley, shows a high tolerance to salt and B levels. In the present study the interaction between B and salt was studied after 20 days of treatment at low (100 mM) and high salinity (430 mM NaCl), assessing changes in nitrogen metabolites and in the activity of key nitrogen-assimilating enzymes. Under non-saline conditions, the presence of excessive B favored higher nitrate and ammonium mobilization to leaves, increasing nitrate reductase (NR) activity but not glutamine synthetase (GS). Thus, the increment of nitrogen use efficiency by B application would contribute partially to maintain the biomass production in this ecotype. Positive relationships between NR activity, nitrate, and stomatal conductance were observed in leaves. The increment of major amino acids alanine and serine would indicate a photoprotective role of photorespiration under low-salinity conditions, thus the inhibition of nitrogen assimilation pathway (NR and GS activities) occurred only at high salinity. The role of cytosolic GS regarding the proline accumulation is discussed.Research funded by The Basque Government (IT932-1). E.I. Bastías received a pre-doctoral fellowship from the Universidad de Tarapacá (Arica, Chile) and financial support by Convenio Desempeño Regional UTA-1795.MDPI2020202020202020info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/42771reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoIngléshttps://www.mdpi.com/2223-7747/9/3/322info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/© 2020 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (http://creativecommons.org/licenses/by/4.0/).oai:addi.ehu.eus:10810/427712026-06-18T09:23:17Z
dc.title.none.fl_str_mv Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
title Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
spellingShingle Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
Fuertes Mendizábal, Teresa
amino acids
glutamine synthetase
nitrate reductase
proline
title_short Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
title_full Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
title_fullStr Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
title_full_unstemmed Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
title_sort Nitrogen Assimilation in the Highly Salt- and Boron-Tolerant Ecotype Zea mays L. Amylacea
dc.creator.none.fl_str_mv Fuertes Mendizábal, Teresa
Bastías, Elizabeth Irica
González Murua, María del Carmen Begoña
González Moro, María Begoña
author Fuertes Mendizábal, Teresa
author_facet Fuertes Mendizábal, Teresa
Bastías, Elizabeth Irica
González Murua, María del Carmen Begoña
González Moro, María Begoña
author_role author
author2 Bastías, Elizabeth Irica
González Murua, María del Carmen Begoña
González Moro, María Begoña
author2_role author
author
author
dc.subject.none.fl_str_mv amino acids
glutamine synthetase
nitrate reductase
proline
topic amino acids
glutamine synthetase
nitrate reductase
proline
description The Lluta Valley in Northern Chile is an important agricultural area affected by both salinity and boron (B) toxicity. <i>Zea mays</i> L. amylacea, an ecotype arisen because of the seed selection practiced in this valley, shows a high tolerance to salt and B levels. In the present study the interaction between B and salt was studied after 20 days of treatment at low (100 mM) and high salinity (430 mM NaCl), assessing changes in nitrogen metabolites and in the activity of key nitrogen-assimilating enzymes. Under non-saline conditions, the presence of excessive B favored higher nitrate and ammonium mobilization to leaves, increasing nitrate reductase (NR) activity but not glutamine synthetase (GS). Thus, the increment of nitrogen use efficiency by B application would contribute partially to maintain the biomass production in this ecotype. Positive relationships between NR activity, nitrate, and stomatal conductance were observed in leaves. The increment of major amino acids alanine and serine would indicate a photoprotective role of photorespiration under low-salinity conditions, thus the inhibition of nitrogen assimilation pathway (NR and GS activities) occurred only at high salinity. The role of cytosolic GS regarding the proline accumulation is discussed.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/42771
url http://hdl.handle.net/10810/42771
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://www.mdpi.com/2223-7747/9/3/322
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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