Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions

Polyaromatic phenanthrene (Phe) and benzo[a]pyrene (BaP) are highly toxic, mutagenic, and carcinogenic contaminants widely dispersed in nature, including saline environments. Polyextremotolerant Rhodotorula mucilaginosa EXF-1630, isolated from Arctic sea ice, was grown on a huge concentration range...

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Autores: Batista-García, Ramón, Martínez-Ávila, Liliana, Peidro-Guzmán, Heidy, Pérez-Llano, Yordanis, Moreno-Perlin, Tonatiuh, Sánchez-Reyes, Ayixon, Fernández-Silva, Arline, Folch, Jorge Luis, Cabana, Hubert, Gunde-Cimerman, Nina, Ángeles, Gabriela, Aranda-Ballesteros, Elizabet
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2021
País:España
Recursos:Universidad de Jaén
Repositório:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
OAI Identifier:oai:ruja.ujaen.es:10953/4539
Acesso em linha:https://hdl.handle.net/10953/4539
Access Level:Acceso aberto
Palavra-chave:Polycyclic aromatic hydrocarbons
Transcriptomic
PAH metabolites
Bioremediation
Halotolerant yeasts
Benzo[a]pyrene
Phenanthrene
Rhodotorula mucilaginosa
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spelling Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditionsBatista-García, RamónMartínez-Ávila, LilianaPeidro-Guzmán, HeidyPérez-Llano, YordanisMoreno-Perlin, TonatiuhSánchez-Reyes, AyixonFernández-Silva, ArlineFolch, Jorge LuisCabana, HubertGunde-Cimerman, NinaÁngeles, GabrielaAranda-Ballesteros, ElizabetPolycyclic aromatic hydrocarbonsTranscriptomicPAH metabolitesBioremediationHalotolerant yeastsBenzo[a]pyrenePhenanthreneRhodotorula mucilaginosaPolyaromatic phenanthrene (Phe) and benzo[a]pyrene (BaP) are highly toxic, mutagenic, and carcinogenic contaminants widely dispersed in nature, including saline environments. Polyextremotolerant Rhodotorula mucilaginosa EXF-1630, isolated from Arctic sea ice, was grown on a huge concentration range -10 to 500 ppm- of Phe and BaP as sole carbon sources at hypersaline conditions (1 M NaCl). Selected polycyclic aromatic hydrocarbons (PAHs) supported growth as well as glucose, even at high PAH concentrations. Initially, up to 40% of Phe and BaP were adsorbed, followed by biodegradation, resulting in 80% removal in 10 days. While extracellular laccase, peroxidase, and un-specific peroxygenase activities were not detected, NADPH-cytochrome c reductase activity peaked at 4 days. The successful removal of PAHs and the absence of toxic metabolites were confirmed by toxicological tests on moss Physcomitrium patens, bacterium Aliivibrio fischeri, human erythrocytes, and pulmonary epithelial cells (A549). Metabolic profiles were determined at the midpoint of the biodegradation exponential phase, with added Phe and BaP (100 ppm) and 1 M NaCl. Different hydroxylated products were found in the culture medium, while the conjugative metabolite 1-phenanthryl-b-D glucopyranose was detected in the medium and in the cells. Transcriptome analysis resulted in 870 upregulated and 2,288 downregulated transcripts on PAHs, in comparison to glucose. Genomic mining of 61 available yeast genomes showed a widespread distribution of 31 xenobiotic degradation pathways in different yeast lineages. Two distributions with similar metabolic capacities included black yeasts and mainly members of the Sporidiobolaceae family (including EXF-1630), respectively. This is the first work describing a metabolic profile and transcriptomic analysis of PAH degradation by yeast.Elsevier202520252021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/10953/4539reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaéninstname:Universidad de JaénInglésEnvironmental Pollution 271 (2021) 116358info:eu-repo/semantics/openAccessoai:ruja.ujaen.es:10953/45392026-06-24T12:41:07Z
dc.title.none.fl_str_mv Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
title Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
spellingShingle Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
Batista-García, Ramón
Polycyclic aromatic hydrocarbons
Transcriptomic
PAH metabolites
Bioremediation
Halotolerant yeasts
Benzo[a]pyrene
Phenanthrene
Rhodotorula mucilaginosa
title_short Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
title_full Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
title_fullStr Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
title_full_unstemmed Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
title_sort Tracking gene expression, metabolic profiles, and biochemical analysis in the halotolerant basidiomycetous yeast Rhodotorula mucilaginosa EXF-1630 during benzo[a]pyrene and phenanthrene biodegradation under hypersaline conditions
dc.creator.none.fl_str_mv Batista-García, Ramón
Martínez-Ávila, Liliana
Peidro-Guzmán, Heidy
Pérez-Llano, Yordanis
Moreno-Perlin, Tonatiuh
Sánchez-Reyes, Ayixon
Fernández-Silva, Arline
Folch, Jorge Luis
Cabana, Hubert
Gunde-Cimerman, Nina
Ángeles, Gabriela
Aranda-Ballesteros, Elizabet
author Batista-García, Ramón
author_facet Batista-García, Ramón
Martínez-Ávila, Liliana
Peidro-Guzmán, Heidy
Pérez-Llano, Yordanis
Moreno-Perlin, Tonatiuh
Sánchez-Reyes, Ayixon
Fernández-Silva, Arline
Folch, Jorge Luis
Cabana, Hubert
Gunde-Cimerman, Nina
Ángeles, Gabriela
Aranda-Ballesteros, Elizabet
author_role author
author2 Martínez-Ávila, Liliana
Peidro-Guzmán, Heidy
Pérez-Llano, Yordanis
Moreno-Perlin, Tonatiuh
Sánchez-Reyes, Ayixon
Fernández-Silva, Arline
Folch, Jorge Luis
Cabana, Hubert
Gunde-Cimerman, Nina
Ángeles, Gabriela
Aranda-Ballesteros, Elizabet
author2_role author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Polycyclic aromatic hydrocarbons
Transcriptomic
PAH metabolites
Bioremediation
Halotolerant yeasts
Benzo[a]pyrene
Phenanthrene
Rhodotorula mucilaginosa
topic Polycyclic aromatic hydrocarbons
Transcriptomic
PAH metabolites
Bioremediation
Halotolerant yeasts
Benzo[a]pyrene
Phenanthrene
Rhodotorula mucilaginosa
description Polyaromatic phenanthrene (Phe) and benzo[a]pyrene (BaP) are highly toxic, mutagenic, and carcinogenic contaminants widely dispersed in nature, including saline environments. Polyextremotolerant Rhodotorula mucilaginosa EXF-1630, isolated from Arctic sea ice, was grown on a huge concentration range -10 to 500 ppm- of Phe and BaP as sole carbon sources at hypersaline conditions (1 M NaCl). Selected polycyclic aromatic hydrocarbons (PAHs) supported growth as well as glucose, even at high PAH concentrations. Initially, up to 40% of Phe and BaP were adsorbed, followed by biodegradation, resulting in 80% removal in 10 days. While extracellular laccase, peroxidase, and un-specific peroxygenase activities were not detected, NADPH-cytochrome c reductase activity peaked at 4 days. The successful removal of PAHs and the absence of toxic metabolites were confirmed by toxicological tests on moss Physcomitrium patens, bacterium Aliivibrio fischeri, human erythrocytes, and pulmonary epithelial cells (A549). Metabolic profiles were determined at the midpoint of the biodegradation exponential phase, with added Phe and BaP (100 ppm) and 1 M NaCl. Different hydroxylated products were found in the culture medium, while the conjugative metabolite 1-phenanthryl-b-D glucopyranose was detected in the medium and in the cells. Transcriptome analysis resulted in 870 upregulated and 2,288 downregulated transcripts on PAHs, in comparison to glucose. Genomic mining of 61 available yeast genomes showed a widespread distribution of 31 xenobiotic degradation pathways in different yeast lineages. Two distributions with similar metabolic capacities included black yeasts and mainly members of the Sporidiobolaceae family (including EXF-1630), respectively. This is the first work describing a metabolic profile and transcriptomic analysis of PAH degradation by yeast.
publishDate 2021
dc.date.none.fl_str_mv 2021
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/10953/4539
url https://hdl.handle.net/10953/4539
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Environmental Pollution 271 (2021) 116358
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
instname:Universidad de Jaén
instname_str Universidad de Jaén
reponame_str RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
collection RUJA. Repositorio Institucional de la Producción Científica de la Universidad de Jaén
repository.name.fl_str_mv
repository.mail.fl_str_mv
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