Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension

Chronic thromboembolic pulmonary hypertension (CTEPH) is a vascular disease characterized by the presence of organized thromboembolic material in pulmonary arteries leading to increased vascular resistance, heart failure and death. Dysfunction of endothelial cells is involved in CTEPH. The present s...

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Autores: Nukala, Sarath Babu, Tura-Ceide, Olga, Aldini, Giancarlo, Françoise Smolders, Valérie, Blanco Vich, Isabel, Peinado, Víctor I., Castellà Pericàs, Manuel, Barberà i Mir, Joan Albert, Altomare, Alessandra, Baron, Giovanna, Carini, Marina, Cascante i Serratosa, Marta, D'Amato, Alfonsina
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/177022
Acesso em linha:https://hdl.handle.net/2445/177022
Access Level:acceso abierto
Palavra-chave:Hipertensió pulmonar
Tromboembolisme
Pulmonary hypertension
Thromboembolism
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spelling Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertensionNukala, Sarath BabuTura-Ceide, OlgaAldini, GiancarloFrançoise Smolders, ValérieBlanco Vich, IsabelPeinado, Víctor I.Castellà Pericàs, ManuelBarberà i Mir, Joan AlbertAltomare, AlessandraBaron, GiovannaCarini, MarinaCascante i Serratosa, MartaD'Amato, AlfonsinaHipertensió pulmonarTromboembolismePulmonary hypertensionThromboembolismChronic thromboembolic pulmonary hypertension (CTEPH) is a vascular disease characterized by the presence of organized thromboembolic material in pulmonary arteries leading to increased vascular resistance, heart failure and death. Dysfunction of endothelial cells is involved in CTEPH. The present study describes for the first time the molecular processes underlying endothelial dysfunction in the development of the CTEPH. The advanced analytical approach and the protein network analyses of patient derived CTEPH endothelial cells allowed the quantitation of 3258 proteins. The 673 differentially regulated proteins were associated with functional and disease protein network modules. The protein network analyses resulted in the characterization of dysregulated pathways associated with endothelial dysfunction, such as mitochondrial dysfunction, oxidative phosphorylation, sirtuin signaling, inflammatory response, oxidative stress and fatty acid metabolism related pathways. In addition, the quantification of advanced oxidation protein products, total protein carbonyl content, and intracellular reactive oxygen species resulted increased attesting the dysregulation of oxidative stress response. In conclusion this is the first quantitative study to highlight the involvement of endothelial dysfunction in CTEPH using patient samples and by network medicine approach.Nature Publishing Group2021info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/177022Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: https://doi.org/10.1038/s41598-021-85004-zScientific Reports, 2021, vol. 11, num. 1, p. 5583https://doi.org/10.1038/s41598-021-85004-zinfo:eu-repo/grantAgreement/EC/H2020/675527cc-by (c) Nukala, Sarath Babu et al., 2021http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1770222026-05-27T06:46:51Z
dc.title.none.fl_str_mv Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
title Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
spellingShingle Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
Nukala, Sarath Babu
Hipertensió pulmonar
Tromboembolisme
Pulmonary hypertension
Thromboembolism
title_short Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
title_full Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
title_fullStr Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
title_full_unstemmed Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
title_sort Protein network analyses of pulmonary endothelial cells in chronic thromboembolic pulmonary hypertension
dc.creator.none.fl_str_mv Nukala, Sarath Babu
Tura-Ceide, Olga
Aldini, Giancarlo
Françoise Smolders, Valérie
Blanco Vich, Isabel
Peinado, Víctor I.
Castellà Pericàs, Manuel
Barberà i Mir, Joan Albert
Altomare, Alessandra
Baron, Giovanna
Carini, Marina
Cascante i Serratosa, Marta
D'Amato, Alfonsina
author Nukala, Sarath Babu
author_facet Nukala, Sarath Babu
Tura-Ceide, Olga
Aldini, Giancarlo
Françoise Smolders, Valérie
Blanco Vich, Isabel
Peinado, Víctor I.
Castellà Pericàs, Manuel
Barberà i Mir, Joan Albert
Altomare, Alessandra
Baron, Giovanna
Carini, Marina
Cascante i Serratosa, Marta
D'Amato, Alfonsina
author_role author
author2 Tura-Ceide, Olga
Aldini, Giancarlo
Françoise Smolders, Valérie
Blanco Vich, Isabel
Peinado, Víctor I.
Castellà Pericàs, Manuel
Barberà i Mir, Joan Albert
Altomare, Alessandra
Baron, Giovanna
Carini, Marina
Cascante i Serratosa, Marta
D'Amato, Alfonsina
author2_role author
author
author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Hipertensió pulmonar
Tromboembolisme
Pulmonary hypertension
Thromboembolism
topic Hipertensió pulmonar
Tromboembolisme
Pulmonary hypertension
Thromboembolism
description Chronic thromboembolic pulmonary hypertension (CTEPH) is a vascular disease characterized by the presence of organized thromboembolic material in pulmonary arteries leading to increased vascular resistance, heart failure and death. Dysfunction of endothelial cells is involved in CTEPH. The present study describes for the first time the molecular processes underlying endothelial dysfunction in the development of the CTEPH. The advanced analytical approach and the protein network analyses of patient derived CTEPH endothelial cells allowed the quantitation of 3258 proteins. The 673 differentially regulated proteins were associated with functional and disease protein network modules. The protein network analyses resulted in the characterization of dysregulated pathways associated with endothelial dysfunction, such as mitochondrial dysfunction, oxidative phosphorylation, sirtuin signaling, inflammatory response, oxidative stress and fatty acid metabolism related pathways. In addition, the quantification of advanced oxidation protein products, total protein carbonyl content, and intracellular reactive oxygen species resulted increased attesting the dysregulation of oxidative stress response. In conclusion this is the first quantitative study to highlight the involvement of endothelial dysfunction in CTEPH using patient samples and by network medicine approach.
publishDate 2021
dc.date.none.fl_str_mv 2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/177022
url https://hdl.handle.net/2445/177022
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1038/s41598-021-85004-z
Scientific Reports, 2021, vol. 11, num. 1, p. 5583
https://doi.org/10.1038/s41598-021-85004-z
info:eu-repo/grantAgreement/EC/H2020/675527
dc.rights.none.fl_str_mv cc-by (c) Nukala, Sarath Babu et al., 2021
http://creativecommons.org/licenses/by/3.0/es
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by (c) Nukala, Sarath Babu et al., 2021
http://creativecommons.org/licenses/by/3.0/es
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Nature Publishing Group
publisher.none.fl_str_mv Nature Publishing Group
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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