Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29

Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 28-02-2014

Detalles Bibliográficos
Autor: Ballesteros Plaza, David
Tipo de recurso: tesis doctoral
Fecha de publicación:2014
País:España
Institución:Universidad Autónoma de Madrid
Repositorio:Biblos-e Archivo. Repositorio Institucional de la UAM
Idioma:español
OAI Identifier:oai:repositorio.uam.es:10486/660213
Acceso en línea:http://hdl.handle.net/10486/660213
Access Level:acceso abierto
Palabra clave:Bacteriófagos - Tesis doctorales
Biología y Biomedicina / Biología
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spelling Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29Ballesteros Plaza, DavidBacteriófagos - Tesis doctoralesBiología y Biomedicina / BiologíaTesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 28-02-2014Trabajo realizado en el Centro de Biología Molecular “Severo Ochoa” (CSIC/UAM), en el grupo de Replicación del DNA del bacteriófago φ29.Bacillus subtilis bacteriophage φ29 is a well-studied virus used as a model organism to expand knowledge in aspects concerning to DNA replication and transcription. However, little is known about the in vivo organization of phage φ29 DNA replication and the proteins involved in this process. Phage φ29 proteins p1 and p17 are known to be required for DNA replication in vivo but their temporal and spatial organizations in infected cells have not yet been determined. Although the role of p17 in viral DNA replication in vivo has been partially elucidated, phage φ29 protein p1 function remained elusive. Protein p1 is a membrane-associated protein that forms large protofilament sheets that resemble eukaryotic tubulin and bacterial FtsZ polymers. In the absence of protein p1, phage φ29 DNA replication is impaired. In this Thesis, it is shown that a functional fusion of protein p1 to YFP localizes at the medial region of B. subtilis cells independently of other phage-encoded proteins. Moreover, protein p1 colocalizes with the B. subtilis cell division protein FtsZ and these proteins are associated in a complex. Importantly, the midcell localization of YFP-p1 was disrupted in a strain that does not express FtsZ, and the fluorescent signal was distributed all over the cell. Depletion of penicillin-binding protein 2B (PBP2B) in B. subtilis cells did not affect the subcellular localization of YFP-p1, indicating that its distribution does not depend on septal wall synthesis. Interestingly, when φ29 protein p1 was expressed, B. subtilis cells were about 1.5-fold longer than wild type cells, and the accumulation of φ29 DNA was higher in mutant B. subtilis cells with increased length. We propose that protein p1 promotes viral DNA replication by increasing the length of B. subtilis cells. By using a bacterial two hybrids system, we have shown that protein p1 associates with protein p17 in vivo. Apart from its role in DNA replication, protein p17 is required for the entrance of viral DNA into the cell during the injection process. In this Thesis, it has been observed that p17 localizes in a helical pattern along the whole B. subtilis cell in the absence of other viral components. Also, this localization does not require the cytoskeletal proteins MreB, Mbl or MreBH. The helix-like localization of p17 and its association with the viral protein p16.7, which also distributes in a helical manner, might reflect another role for protein p17, stabilizing p16.7 to redistribute viral genomes at the final stages of φ29 DNA replication.Salas Falgueras, MargaritaMuñoz Espín, DanielDepartamento de Biología MolecularFacultad de CienciasCentro de Biología Molecular Severo Ochoa (CBM)20142014-02-28doctoral thesishttp://purl.org/coar/resource_type/c_db06NAhttp://purl.org/coar/version/c_be7fb7dd8ff6fe43info:eu-repo/semantics/doctoralThesisapplication/pdfhttp://hdl.handle.net/10486/660213reponame:Biblos-e Archivo. Repositorio Institucional de la UAMinstname:Universidad Autónoma de MadridEspañolspaopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:repositorio.uam.es:10486/6602132026-06-23T12:46:27Z
dc.title.none.fl_str_mv Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
title Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
spellingShingle Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
Ballesteros Plaza, David
Bacteriófagos - Tesis doctorales
Biología y Biomedicina / Biología
title_short Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
title_full Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
title_fullStr Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
title_full_unstemmed Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
title_sort Estudio funcional de las proteínas p1 y p17 del bacteriófago phi 29
dc.creator.none.fl_str_mv Ballesteros Plaza, David
author Ballesteros Plaza, David
author_facet Ballesteros Plaza, David
author_role author
dc.contributor.none.fl_str_mv Salas Falgueras, Margarita
Muñoz Espín, Daniel
Departamento de Biología Molecular
Facultad de Ciencias
Centro de Biología Molecular Severo Ochoa (CBM)
dc.subject.none.fl_str_mv Bacteriófagos - Tesis doctorales
Biología y Biomedicina / Biología
topic Bacteriófagos - Tesis doctorales
Biología y Biomedicina / Biología
description Tesis Doctoral inédita leída en la Universidad Autónoma de Madrid, Facultad de Ciencias, Departamento de Biología Molecular. Fecha de lectura: 28-02-2014
publishDate 2014
dc.date.none.fl_str_mv 2014
2014-02-28
dc.type.none.fl_str_mv doctoral thesis
http://purl.org/coar/resource_type/c_db06
NA
http://purl.org/coar/version/c_be7fb7dd8ff6fe43
dc.type.openaire.fl_str_mv info:eu-repo/semantics/doctoralThesis
format doctoralThesis
dc.identifier.none.fl_str_mv http://hdl.handle.net/10486/660213
url http://hdl.handle.net/10486/660213
dc.language.none.fl_str_mv Español
spa
language_invalid_str_mv Español
language spa
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Biblos-e Archivo. Repositorio Institucional de la UAM
instname:Universidad Autónoma de Madrid
instname_str Universidad Autónoma de Madrid
reponame_str Biblos-e Archivo. Repositorio Institucional de la UAM
collection Biblos-e Archivo. Repositorio Institucional de la UAM
repository.name.fl_str_mv
repository.mail.fl_str_mv
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