Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors

This work reports for the first time an electrochemical immunosensor for the determination of adrenocorticotropin hormone (ACTH). The immunoelectrode design involves the use of amino phenylboronic acid for the oriented immobilization of anti-ACTH antibodies onto screen-printed carbon modified electr...

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Detalles Bibliográficos
Autores: Moreno Guzmán, María, Villalonga Santana, Reynaldo, Ojeda Fernández, Irene, González Cortés, Araceli, Yáñez-Sedeño Orive, Paloma, Pingarrón Carrazón, José Manuel
Tipo de recurso: artículo
Fecha de publicación:2012
País:España
Institución:Universidad Complutense de Madrid (UCM)
Repositorio:Docta Complutense
Idioma:inglés
OAI Identifier:oai:docta.ucm.es:20.500.14352/42874
Acceso en línea:https://hdl.handle.net/20.500.14352/42874
Access Level:acceso abierto
Palabra clave:543
Adrenocorticotropin hormone
Phenyl boronic acid
Electrochemical immunosensor
Química analítica (Química)
2301 Química Analítica
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oai_identifier_str oai:docta.ucm.es:20.500.14352/42874
network_acronym_str ES
network_name_str España
repository_id_str
spelling Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensorsMoreno Guzmán, MaríaVillalonga Santana, ReynaldoOjeda Fernández, IreneGonzález Cortés, AraceliYáñez-Sedeño Orive, PalomaPingarrón Carrazón, José Manuel543Adrenocorticotropin hormonePhenyl boronic acidElectrochemical immunosensorQuímica analítica (Química)2301 Química AnalíticaThis work reports for the first time an electrochemical immunosensor for the determination of adrenocorticotropin hormone (ACTH). The immunoelectrode design involves the use of amino phenylboronic acid for the oriented immobilization of anti-ACTH antibodies onto screen-printed carbon modified electrode surfaces. A competitive immunoassay between the antigen and the biotinylated hormone for the binding sites of the immobilized antibody was performed. The electroanalytical response was generated by using alkaline phosphatase-labelled streptavidin and 1-naphtyl phosphate as the enzyme substrate. The electrochemical oxidation of the enzyme reaction product, 1-naphtol, measured by differential pulse voltammetry was employed to monitor the affinity reaction. Under the optimized working conditions, an extremely low detection limit of 18 pg/L was obtained. Cross-reactivity was evaluated against other hormones (cortisol, estradiol, testosterone, progesterone, hGH and prolactin) and the obtained results demonstrated an excellent selectivity. The developed immunosensor was applied to a human serum sample containing a certified amount of ACTH with good results.ElsevierUniversidad Complutense de Madrid20122012-01-0120122012-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/20.500.14352/42874reponame:Docta Complutenseinstname:Universidad Complutense de Madrid (UCM)Inglésengopen accesshttp://purl.org/coar/access_right/c_abf2Atribución-NoComercial-SinDerivadas 3.0 Españahttps://creativecommons.org/licenses/by-nc-nd/3.0/es/info:eu-repo/semantics/openAccessoai:docta.ucm.es:20.500.14352/428742026-06-02T12:44:21Z
dc.title.none.fl_str_mv Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
title Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
spellingShingle Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
Moreno Guzmán, María
543
Adrenocorticotropin hormone
Phenyl boronic acid
Electrochemical immunosensor
Química analítica (Química)
2301 Química Analítica
title_short Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
title_full Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
title_fullStr Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
title_full_unstemmed Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
title_sort Ultrasensitive detection of adrenocorticotropin hormone (ACTH) using disposable phenylboronic-modified electrochemical immunosensors
dc.creator.none.fl_str_mv Moreno Guzmán, María
Villalonga Santana, Reynaldo
Ojeda Fernández, Irene
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author Moreno Guzmán, María
author_facet Moreno Guzmán, María
Villalonga Santana, Reynaldo
Ojeda Fernández, Irene
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author_role author
author2 Villalonga Santana, Reynaldo
Ojeda Fernández, Irene
González Cortés, Araceli
Yáñez-Sedeño Orive, Paloma
Pingarrón Carrazón, José Manuel
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Universidad Complutense de Madrid
dc.subject.none.fl_str_mv 543
Adrenocorticotropin hormone
Phenyl boronic acid
Electrochemical immunosensor
Química analítica (Química)
2301 Química Analítica
topic 543
Adrenocorticotropin hormone
Phenyl boronic acid
Electrochemical immunosensor
Química analítica (Química)
2301 Química Analítica
description This work reports for the first time an electrochemical immunosensor for the determination of adrenocorticotropin hormone (ACTH). The immunoelectrode design involves the use of amino phenylboronic acid for the oriented immobilization of anti-ACTH antibodies onto screen-printed carbon modified electrode surfaces. A competitive immunoassay between the antigen and the biotinylated hormone for the binding sites of the immobilized antibody was performed. The electroanalytical response was generated by using alkaline phosphatase-labelled streptavidin and 1-naphtyl phosphate as the enzyme substrate. The electrochemical oxidation of the enzyme reaction product, 1-naphtol, measured by differential pulse voltammetry was employed to monitor the affinity reaction. Under the optimized working conditions, an extremely low detection limit of 18 pg/L was obtained. Cross-reactivity was evaluated against other hormones (cortisol, estradiol, testosterone, progesterone, hGH and prolactin) and the obtained results demonstrated an excellent selectivity. The developed immunosensor was applied to a human serum sample containing a certified amount of ACTH with good results.
publishDate 2012
dc.date.none.fl_str_mv 2012
2012-01-01
2012
2012-01-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/20.500.14352/42874
url https://hdl.handle.net/20.500.14352/42874
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Atribución-NoComercial-SinDerivadas 3.0 España
https://creativecommons.org/licenses/by-nc-nd/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:Docta Complutense
instname:Universidad Complutense de Madrid (UCM)
instname_str Universidad Complutense de Madrid (UCM)
reponame_str Docta Complutense
collection Docta Complutense
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869412715032739840
score 15,301629

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