Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?

The severity of canine leishmaniosis (CanL) due to Leishmania infantum might be affected by other vector-borne organisms that mimic its clinical signs and clinicopathological abnormalities. The aim of this study was to determine co-infections with other vector-borne pathogens based on serological an...

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Autores: Baxarias, Marta|||0000-0002-1830-4366, Álvarez-Fernández, Alejandra, Martínez-Orellana, Pamela|||0000-0002-2025-9445, Montserrat-Sangrà, Sara, Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358, Rojas, Alicia, Nachum-Biala, Yaarit, Baneth, Gad|||0000-0002-7549-1305, Solano Gallego, Laia|||0000-0001-8479-4896
Tipo de documento: artigo
Data de publicação:2018
País:España
Recursos:Universitat Autònoma de Barcelona
Repositório:Dipòsit Digital de Documents de la UAB
Idioma:inglês
OAI Identifier:oai:ddd.uab.cat:190763
Acesso em linha:https://ddd.uab.cat/record/190763
https://dx.doi.org/urn:doi:10.1186/s13071-018-2724-9
Access Level:Acceso aberto
Palavra-chave:Canine leishmaniosis
Spain
Leishmania infantum
Rickettsia conorii
Ehrlichia canis
Anaplasma phagocytophilum
Anaplasma platys
Hepatozoon canis
Bartonella henselae
Co-infection
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network_name_str España
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spelling Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?Baxarias, Marta|||0000-0002-1830-4366Álvarez-Fernández, AlejandraMartínez-Orellana, Pamela|||0000-0002-2025-9445Montserrat-Sangrà, SaraOrdeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358Rojas, AliciaNachum-Biala, YaaritBaneth, Gad|||0000-0002-7549-1305Solano Gallego, Laia|||0000-0001-8479-4896Canine leishmaniosisSpainLeishmania infantumRickettsia conoriiEhrlichia canisAnaplasma phagocytophilumAnaplasma platysHepatozoon canisBartonella henselaeCo-infectionThe severity of canine leishmaniosis (CanL) due to Leishmania infantum might be affected by other vector-borne organisms that mimic its clinical signs and clinicopathological abnormalities. The aim of this study was to determine co-infections with other vector-borne pathogens based on serological and molecular techniques in dogs with clinical leishmaniosis living in Spain and to associate them with clinical signs and clinicopathological abnormalities as well as disease severity. Sixty-one dogs with clinical leishmaniosis and 16 apparently healthy dogs were tested for Rickettsia conorii, Ehrlichia canis, Anaplasma phagocytophilum and Bartonella henselae antigens by the immunofluorescence antibody test (IFAT) and for E. canis, Anaplasma spp., Hepatozoon spp., Babesia spp. and filarioid DNA by polymerase chain reaction (PCR). Among the dogs examined by IFAT, the seroprevalences were: 69% for R. conorii, 57% for E. canis, 44% for A. phagocytophilum and 37% for B. henselae ; while the prevalences found by PCR were: 8% for Ehrlichia / Anaplasma, 3% for Anaplasma platys and 1% for H. canis. No other pathogen DNA was detected. Statistical association was found between dogs with clinical leishmaniosis and seroreactivity to R. conorii antigen (Fisher's exact test: P = 0.025, OR = 4.1, 95% CI = 1-17) and A. phagocytophilum antigen (Fisher's exact test: P = 0.002, OR = 14.3, 95% CI = 2-626) and being positive to more than one serological or molecular tests (co-infections) (Mann-Whitney test: U = 243, Z = -2.6, n = 14, n = 61, P = 0.01) when compared with healthy dogs. Interestingly, a statistical association was found between the presence of R. conorii, E. canis, A. phagocytophilum and B. henselae antibodies in sick dogs and some clinicopathological abnormalities such as albumin and albumin/globulin ratio decrease and increase in serum globulins. Furthermore, seroreactivity with A. phagocytophilum antigens was statistically associated with CanL clinical stages III and IV. This study demonstrates that dogs with clinical leishmaniosis from Catalonia (Spain) have a higher rate of co-infections with other vector-borne pathogens when compared with healthy controls. Furthermore, positivity to some vector-borne pathogens was associated with more marked clinicopathological abnormalities as well as disease severity with CanL. 22018-01-0120182018-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/190763https://dx.doi.org/urn:doi:10.1186/s13071-018-2724-9reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengMinisterio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2012-32498Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2015-68477open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:1907632026-06-06T12:50:31Z
dc.title.none.fl_str_mv Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
title Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
spellingShingle Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
Baxarias, Marta|||0000-0002-1830-4366
Canine leishmaniosis
Spain
Leishmania infantum
Rickettsia conorii
Ehrlichia canis
Anaplasma phagocytophilum
Anaplasma platys
Hepatozoon canis
Bartonella henselae
Co-infection
title_short Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
title_full Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
title_fullStr Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
title_full_unstemmed Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
title_sort Does co-infection with vector-borne pathogens play a role in clinical canine leishmaniosis?
dc.creator.none.fl_str_mv Baxarias, Marta|||0000-0002-1830-4366
Álvarez-Fernández, Alejandra
Martínez-Orellana, Pamela|||0000-0002-2025-9445
Montserrat-Sangrà, Sara
Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358
Rojas, Alicia
Nachum-Biala, Yaarit
Baneth, Gad|||0000-0002-7549-1305
Solano Gallego, Laia|||0000-0001-8479-4896
author Baxarias, Marta|||0000-0002-1830-4366
author_facet Baxarias, Marta|||0000-0002-1830-4366
Álvarez-Fernández, Alejandra
Martínez-Orellana, Pamela|||0000-0002-2025-9445
Montserrat-Sangrà, Sara
Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358
Rojas, Alicia
Nachum-Biala, Yaarit
Baneth, Gad|||0000-0002-7549-1305
Solano Gallego, Laia|||0000-0001-8479-4896
author_role author
author2 Álvarez-Fernández, Alejandra
Martínez-Orellana, Pamela|||0000-0002-2025-9445
Montserrat-Sangrà, Sara
Ordeix, Laura (Ordeix i Esteve)|||0000-0002-3631-8358
Rojas, Alicia
Nachum-Biala, Yaarit
Baneth, Gad|||0000-0002-7549-1305
Solano Gallego, Laia|||0000-0001-8479-4896
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Canine leishmaniosis
Spain
Leishmania infantum
Rickettsia conorii
Ehrlichia canis
Anaplasma phagocytophilum
Anaplasma platys
Hepatozoon canis
Bartonella henselae
Co-infection
topic Canine leishmaniosis
Spain
Leishmania infantum
Rickettsia conorii
Ehrlichia canis
Anaplasma phagocytophilum
Anaplasma platys
Hepatozoon canis
Bartonella henselae
Co-infection
description The severity of canine leishmaniosis (CanL) due to Leishmania infantum might be affected by other vector-borne organisms that mimic its clinical signs and clinicopathological abnormalities. The aim of this study was to determine co-infections with other vector-borne pathogens based on serological and molecular techniques in dogs with clinical leishmaniosis living in Spain and to associate them with clinical signs and clinicopathological abnormalities as well as disease severity. Sixty-one dogs with clinical leishmaniosis and 16 apparently healthy dogs were tested for Rickettsia conorii, Ehrlichia canis, Anaplasma phagocytophilum and Bartonella henselae antigens by the immunofluorescence antibody test (IFAT) and for E. canis, Anaplasma spp., Hepatozoon spp., Babesia spp. and filarioid DNA by polymerase chain reaction (PCR). Among the dogs examined by IFAT, the seroprevalences were: 69% for R. conorii, 57% for E. canis, 44% for A. phagocytophilum and 37% for B. henselae ; while the prevalences found by PCR were: 8% for Ehrlichia / Anaplasma, 3% for Anaplasma platys and 1% for H. canis. No other pathogen DNA was detected. Statistical association was found between dogs with clinical leishmaniosis and seroreactivity to R. conorii antigen (Fisher's exact test: P = 0.025, OR = 4.1, 95% CI = 1-17) and A. phagocytophilum antigen (Fisher's exact test: P = 0.002, OR = 14.3, 95% CI = 2-626) and being positive to more than one serological or molecular tests (co-infections) (Mann-Whitney test: U = 243, Z = -2.6, n = 14, n = 61, P = 0.01) when compared with healthy dogs. Interestingly, a statistical association was found between the presence of R. conorii, E. canis, A. phagocytophilum and B. henselae antibodies in sick dogs and some clinicopathological abnormalities such as albumin and albumin/globulin ratio decrease and increase in serum globulins. Furthermore, seroreactivity with A. phagocytophilum antigens was statistically associated with CanL clinical stages III and IV. This study demonstrates that dogs with clinical leishmaniosis from Catalonia (Spain) have a higher rate of co-infections with other vector-borne pathogens when compared with healthy controls. Furthermore, positivity to some vector-borne pathogens was associated with more marked clinicopathological abnormalities as well as disease severity with CanL.
publishDate 2018
dc.date.none.fl_str_mv 2
2018-01-01
2018
2018-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/190763
https://dx.doi.org/urn:doi:10.1186/s13071-018-2724-9
url https://ddd.uab.cat/record/190763
https://dx.doi.org/urn:doi:10.1186/s13071-018-2724-9
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2012-32498
Ministerio de Economía y Competitividad https://doi.org/10.13039/501100003329 AGL2015-68477
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.301629

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