Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus

Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. A cross-sectional, descriptive, multicentre study was performed o...

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Autores: Attipa, Charalampos|||0000-0001-6039-6586, Papasouliotis, Kostas, Solano Gallego, Laia|||0000-0001-8479-4896, Baneth, Gad|||0000-0002-7549-1305, Nachum-Biala, Yaarit, Sarvani, Elpida, Knowles, Toby G., Mengi, Sena, Morris, David, Helps, Chris, Tasker, Séverine|||0000-0002-4059-1402
Tipo de recurso: artículo
Fecha de publicación:2017
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:253831
Acceso en línea:https://ddd.uab.cat/record/253831
https://dx.doi.org/urn:doi:10.1186/s13071-017-2063-2
Access Level:acceso abierto
Palabra clave:Cyprus
Feline vector-borne pathogens
Leishmania infantum
Bartonella henselae
Anaplasma platys
Hepatozoon felis
Haemoplasma
FeLV
FIV
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spelling Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from CyprusAttipa, Charalampos|||0000-0001-6039-6586Papasouliotis, KostasSolano Gallego, Laia|||0000-0001-8479-4896Baneth, Gad|||0000-0002-7549-1305Nachum-Biala, YaaritSarvani, ElpidaKnowles, Toby G.Mengi, SenaMorris, DavidHelps, ChrisTasker, Séverine|||0000-0002-4059-1402CyprusFeline vector-borne pathogensLeishmania infantumBartonella henselaeAnaplasma platysHepatozoon felisHaemoplasmaFeLVFIVFeline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. A cross-sectional, descriptive, multicentre study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus. Real-time quantitative polymerase chain reaction (qPCR) assays were used to detect Mycoplasma haemofelis (Mhf), " Candidatus Mycoplasma haemominutum" (CMhm) and " Candidatus Mycoplasma turicensis" (CMt). The population was tested for four FVBP including Bartonella henselae and Leishmania spp. using qPCR, while conventional PCR assays were used to detect Ehrlichia/Anaplasma spp. and Hepatozoon spp. Serological assays were performed to detect Leishmania infantum antibodies, feline leukaemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies. Statistical analysis was performed to test associations and possible risk factors between variables and infectious agents. Ninety-six (55.2%) of the 174 cats were PCR-positive for at least one infectious agent. Forty-six cats (26.4%) were haemoplasma positive, including 13 (7.5%) for Mhf, 36 (20.7%) for CMhm and 12 (6.9%) for CMt. Sixty-six cats (37.9%) were positive for Hepatozoon spp., while 19 (10.9%) were positive for B. henselae, four (2.3%) for Leishmania spp. and one (0.6%) for Ehrlichia/Anaplasma spp. Sequencing revealed the presence of Hepatozoon felis, L. infantum and Anaplasma platys. Of the 164 cats that underwent retroviral serology, 10 (6.1%) were FeLV-positive and 31 (18.9%) were FIV-positive, while L. infantum serology was positive in 7 (4.4%) of the 160 cats tested. Multivariable logistic regression revealed significant associations for various infectious agents including L. infantum with each of Hepatozoon spp. and CMt infection. A high prevalence of infectious agents was found in cats from Cyprus with Mhf, CMhm, CMt, L. infantum, B. henselae, H. felis, A. platys, FeLV and FIV infections reported for the first time. The significant associations between different pathogens provide a better understanding of similarities in the epidemiology of these pathogens and interactions between them. The online version of this article (doi:10.1186/s13071-017-2063-2) contains supplementary material, which is available to authorized users. 22017-01-0120172017-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/253831https://dx.doi.org/urn:doi:10.1186/s13071-017-2063-2reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengopen accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, la comunicació pública de l'obra i la creació d'obres derivades, fins i tot amb finalitats comercials, sempre i quan es reconegui l'autoria de l'obra original.https://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2538312026-06-06T12:50:31Z
dc.title.none.fl_str_mv Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
title Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
spellingShingle Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
Attipa, Charalampos|||0000-0001-6039-6586
Cyprus
Feline vector-borne pathogens
Leishmania infantum
Bartonella henselae
Anaplasma platys
Hepatozoon felis
Haemoplasma
FeLV
FIV
title_short Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
title_full Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
title_fullStr Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
title_full_unstemmed Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
title_sort Prevalence study and risk factor analysis of selected bacterial, protozoal and viral, including vector-borne, pathogens in cats from Cyprus
dc.creator.none.fl_str_mv Attipa, Charalampos|||0000-0001-6039-6586
Papasouliotis, Kostas
Solano Gallego, Laia|||0000-0001-8479-4896
Baneth, Gad|||0000-0002-7549-1305
Nachum-Biala, Yaarit
Sarvani, Elpida
Knowles, Toby G.
Mengi, Sena
Morris, David
Helps, Chris
Tasker, Séverine|||0000-0002-4059-1402
author Attipa, Charalampos|||0000-0001-6039-6586
author_facet Attipa, Charalampos|||0000-0001-6039-6586
Papasouliotis, Kostas
Solano Gallego, Laia|||0000-0001-8479-4896
Baneth, Gad|||0000-0002-7549-1305
Nachum-Biala, Yaarit
Sarvani, Elpida
Knowles, Toby G.
Mengi, Sena
Morris, David
Helps, Chris
Tasker, Séverine|||0000-0002-4059-1402
author_role author
author2 Papasouliotis, Kostas
Solano Gallego, Laia|||0000-0001-8479-4896
Baneth, Gad|||0000-0002-7549-1305
Nachum-Biala, Yaarit
Sarvani, Elpida
Knowles, Toby G.
Mengi, Sena
Morris, David
Helps, Chris
Tasker, Séverine|||0000-0002-4059-1402
author2_role author
author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Cyprus
Feline vector-borne pathogens
Leishmania infantum
Bartonella henselae
Anaplasma platys
Hepatozoon felis
Haemoplasma
FeLV
FIV
topic Cyprus
Feline vector-borne pathogens
Leishmania infantum
Bartonella henselae
Anaplasma platys
Hepatozoon felis
Haemoplasma
FeLV
FIV
description Feline infectious agent studies are lacking in Cyprus. The aims of this study were to determine the prevalence and risk factors for various feline infectious agents, including feline vector-borne pathogens (FVBP), in cats from Cyprus. A cross-sectional, descriptive, multicentre study was performed on 174 feline samples [138 owned and 36 shelter-feral, including both healthy (43) and non-healthy (131), cats] from private veterinary clinics from all six districts of Cyprus. Real-time quantitative polymerase chain reaction (qPCR) assays were used to detect Mycoplasma haemofelis (Mhf), " Candidatus Mycoplasma haemominutum" (CMhm) and " Candidatus Mycoplasma turicensis" (CMt). The population was tested for four FVBP including Bartonella henselae and Leishmania spp. using qPCR, while conventional PCR assays were used to detect Ehrlichia/Anaplasma spp. and Hepatozoon spp. Serological assays were performed to detect Leishmania infantum antibodies, feline leukaemia virus (FeLV) antigen and feline immunodeficiency virus (FIV) antibodies. Statistical analysis was performed to test associations and possible risk factors between variables and infectious agents. Ninety-six (55.2%) of the 174 cats were PCR-positive for at least one infectious agent. Forty-six cats (26.4%) were haemoplasma positive, including 13 (7.5%) for Mhf, 36 (20.7%) for CMhm and 12 (6.9%) for CMt. Sixty-six cats (37.9%) were positive for Hepatozoon spp., while 19 (10.9%) were positive for B. henselae, four (2.3%) for Leishmania spp. and one (0.6%) for Ehrlichia/Anaplasma spp. Sequencing revealed the presence of Hepatozoon felis, L. infantum and Anaplasma platys. Of the 164 cats that underwent retroviral serology, 10 (6.1%) were FeLV-positive and 31 (18.9%) were FIV-positive, while L. infantum serology was positive in 7 (4.4%) of the 160 cats tested. Multivariable logistic regression revealed significant associations for various infectious agents including L. infantum with each of Hepatozoon spp. and CMt infection. A high prevalence of infectious agents was found in cats from Cyprus with Mhf, CMhm, CMt, L. infantum, B. henselae, H. felis, A. platys, FeLV and FIV infections reported for the first time. The significant associations between different pathogens provide a better understanding of similarities in the epidemiology of these pathogens and interactions between them. The online version of this article (doi:10.1186/s13071-017-2063-2) contains supplementary material, which is available to authorized users.
publishDate 2017
dc.date.none.fl_str_mv 2
2017-01-01
2017
2017-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/253831
https://dx.doi.org/urn:doi:10.1186/s13071-017-2063-2
url https://ddd.uab.cat/record/253831
https://dx.doi.org/urn:doi:10.1186/s13071-017-2063-2
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
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https://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
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dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
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