Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones
Antibiotic resistance in Streptococcus pneumoniae has increased worldwide by the spread of a few clones. Fluoroquinolone resistance occurs mainly by alteration of their intracellular targets, the type II DNA topoisomerases, which is acquired either by point mutation or by recombination. Increase in...
| Autores: | , , , , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Estado: | Versión publicada |
| Fecha de publicación: | 2014 |
| País: | España |
| Institución: | Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
| Repositorio: | Recercat. Dipósit de la Recerca de Catalunya |
| OAI Identifier: | oai:recercat.cat:2445/66250 |
| Acceso en línea: | https://hdl.handle.net/2445/66250 |
| Access Level: | acceso abierto |
| Palabra clave: | Pneumococs Estreptococs Resistència als medicaments Malalties de l'aparell respiratori Malalties cròniques Streptococcus pneumonia Streptococcus Drug resistance Respiratory organs diseases Chronic diseases |
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Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clonesLópez, ElenaDomenech Pena, ArnauFerrándiz, María JoséFrias, Maria JoaoArdanuy Tisaire, María CarmenRamirez, MarioGarcía, ErnestoLiñares Louzao, JosefinaCampa, Adela G. de laPneumococsEstreptococsResistència als medicamentsMalalties de l'aparell respiratoriMalalties cròniquesStreptococcus pneumoniaStreptococcusDrug resistanceRespiratory organs diseasesChronic diseasesAntibiotic resistance in Streptococcus pneumoniae has increased worldwide by the spread of a few clones. Fluoroquinolone resistance occurs mainly by alteration of their intracellular targets, the type II DNA topoisomerases, which is acquired either by point mutation or by recombination. Increase in fluoroquinolone-resistance may depend on the balance between antibiotic consumption and the cost that resistance imposes to bacterial fitness. In addition, pneumococcal prophages could play an important role. Prophage induction by fluoroquinolones was confirmed in 4 clinical isolates by using Southern blot hybridization. Clinical isolates (105 fluoroquinolone-resistant and 160 fluoroquinolone-susceptible) were tested for lysogeny by using a PCR assay and functional prophage carriage was studied by mitomycin C induction. Fluoroquinolone-resistant strains harbored fewer inducible prophages (17/43) than fluoroquinolone-susceptible strains (49/70) (P = 0.0018). In addition, isolates of clones associated with fluoroquinolone resistance [CC156 (3/25); CC63 (2/20), and CC81 (1/19)], had lower frequency of functional prophages than isolates of clones with low incidence of fluoroquinolone resistance [CC30 (4/21), CC230 (5/20), CC62 (9/21), and CC180 (21/30)]. Likewise, persistent strains from patients with chronic respiratory diseases subjected to fluoroquinolone treatment had a low frequency of inducible prophages (1/11). Development of ciprofloxacin resistance was tested with two isogenic strains, one lysogenic and the other non-lysogenic: emergence of resistance was only observed in the non-lysogenic strain. These results are compatible with the lysis of lysogenic isolates receiving fluoroquinolones before the development of resistance and explain the inverse relation between presence of inducible prophages and fluoroquinolone-resistance.Public Library of Science (PLoS)2015201520142015info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion8 p.application/pdfapplication/pdfhttps://hdl.handle.net/2445/66250Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0094358PLoS One, 2014, vol. 9, num. 4, p. e94358http://dx.doi.org/10.1371/journal.pone.0094358info:eu-repo/grantAgreement/EC/H2020/692022info:eu-repo/grantAgreement/EC/H2020/667824cc-by (c) López, Elena et al., 2014http://creativecommons.org/licenses/by/3.0/esinfo:eu-repo/semantics/openAccessoai:recercat.cat:2445/662502026-05-29T05:05:01Z |
| dc.title.none.fl_str_mv |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| title |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| spellingShingle |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones López, Elena Pneumococs Estreptococs Resistència als medicaments Malalties de l'aparell respiratori Malalties cròniques Streptococcus pneumonia Streptococcus Drug resistance Respiratory organs diseases Chronic diseases |
| title_short |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| title_full |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| title_fullStr |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| title_full_unstemmed |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| title_sort |
Induction of prophages by fluoroquinolones in streptococcus pneumoniae: implications for emergence of resistance in genetically-related clones |
| dc.creator.none.fl_str_mv |
López, Elena Domenech Pena, Arnau Ferrándiz, María José Frias, Maria Joao Ardanuy Tisaire, María Carmen Ramirez, Mario García, Ernesto Liñares Louzao, Josefina Campa, Adela G. de la |
| author |
López, Elena |
| author_facet |
López, Elena Domenech Pena, Arnau Ferrándiz, María José Frias, Maria Joao Ardanuy Tisaire, María Carmen Ramirez, Mario García, Ernesto Liñares Louzao, Josefina Campa, Adela G. de la |
| author_role |
author |
| author2 |
Domenech Pena, Arnau Ferrándiz, María José Frias, Maria Joao Ardanuy Tisaire, María Carmen Ramirez, Mario García, Ernesto Liñares Louzao, Josefina Campa, Adela G. de la |
| author2_role |
author author author author author author author author |
| dc.subject.none.fl_str_mv |
Pneumococs Estreptococs Resistència als medicaments Malalties de l'aparell respiratori Malalties cròniques Streptococcus pneumonia Streptococcus Drug resistance Respiratory organs diseases Chronic diseases |
| topic |
Pneumococs Estreptococs Resistència als medicaments Malalties de l'aparell respiratori Malalties cròniques Streptococcus pneumonia Streptococcus Drug resistance Respiratory organs diseases Chronic diseases |
| description |
Antibiotic resistance in Streptococcus pneumoniae has increased worldwide by the spread of a few clones. Fluoroquinolone resistance occurs mainly by alteration of their intracellular targets, the type II DNA topoisomerases, which is acquired either by point mutation or by recombination. Increase in fluoroquinolone-resistance may depend on the balance between antibiotic consumption and the cost that resistance imposes to bacterial fitness. In addition, pneumococcal prophages could play an important role. Prophage induction by fluoroquinolones was confirmed in 4 clinical isolates by using Southern blot hybridization. Clinical isolates (105 fluoroquinolone-resistant and 160 fluoroquinolone-susceptible) were tested for lysogeny by using a PCR assay and functional prophage carriage was studied by mitomycin C induction. Fluoroquinolone-resistant strains harbored fewer inducible prophages (17/43) than fluoroquinolone-susceptible strains (49/70) (P = 0.0018). In addition, isolates of clones associated with fluoroquinolone resistance [CC156 (3/25); CC63 (2/20), and CC81 (1/19)], had lower frequency of functional prophages than isolates of clones with low incidence of fluoroquinolone resistance [CC30 (4/21), CC230 (5/20), CC62 (9/21), and CC180 (21/30)]. Likewise, persistent strains from patients with chronic respiratory diseases subjected to fluoroquinolone treatment had a low frequency of inducible prophages (1/11). Development of ciprofloxacin resistance was tested with two isogenic strains, one lysogenic and the other non-lysogenic: emergence of resistance was only observed in the non-lysogenic strain. These results are compatible with the lysis of lysogenic isolates receiving fluoroquinolones before the development of resistance and explain the inverse relation between presence of inducible prophages and fluoroquinolone-resistance. |
| publishDate |
2014 |
| dc.date.none.fl_str_mv |
2014 2015 2015 2015 |
| dc.type.none.fl_str_mv |
info:eu-repo/semantics/article info:eu-repo/semantics/publishedVersion |
| format |
article |
| status_str |
publishedVersion |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/2445/66250 |
| url |
https://hdl.handle.net/2445/66250 |
| dc.language.none.fl_str_mv |
Inglés |
| language_invalid_str_mv |
Inglés |
| dc.relation.none.fl_str_mv |
Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pone.0094358 PLoS One, 2014, vol. 9, num. 4, p. e94358 http://dx.doi.org/10.1371/journal.pone.0094358 info:eu-repo/grantAgreement/EC/H2020/692022 info:eu-repo/grantAgreement/EC/H2020/667824 |
| dc.rights.none.fl_str_mv |
cc-by (c) López, Elena et al., 2014 http://creativecommons.org/licenses/by/3.0/es info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
cc-by (c) López, Elena et al., 2014 http://creativecommons.org/licenses/by/3.0/es |
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openAccess |
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8 p. application/pdf application/pdf |
| dc.publisher.none.fl_str_mv |
Public Library of Science (PLoS) |
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Public Library of Science (PLoS) |
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Articles publicats en revistes (Patologia i Terapèutica Experimental) reponame:Recercat. Dipósit de la Recerca de Catalunya instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya) |
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Recercat. Dipósit de la Recerca de Catalunya |
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Recercat. Dipósit de la Recerca de Catalunya |
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