Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer

Background: colorectal cancer is the third cancer cause of death in Spain. It is important to investigate new tumoral markers for early diagnosis, disease monitoring and prevention strategies. Telomeres protect the chromosome from degradation by nucleases and endto- end fusion. The progressive loss...

Descripción completa

Detalles Bibliográficos
Autores: Valls i Bautista, Cristina, Piñol Felis, Carme, Reñé Espinet, Josep Maria, Buenestado Garcia, Juan, Viñas Salas, Joan
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2012
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10459.1/49253
Acceso en línea:https://doi.org/10.4321/S1130-01082012001000005
http://hdl.handle.net/10459.1/49253
Access Level:acceso abierto
Palabra clave:Telomeric repeat factor 1
Telomere length
Colorectal cancer
Telomerase activity
id ES_8199d46fffb58f9d4721b111ae8ec294
oai_identifier_str oai:recercat.cat:10459.1/49253
network_acronym_str ES
network_name_str España
repository_id_str
spelling Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancerValls i Bautista, CristinaPiñol Felis, CarmeReñé Espinet, Josep MariaBuenestado Garcia, JuanViñas Salas, JoanTelomeric repeat factor 1Telomere lengthColorectal cancerTelomerase activityBackground: colorectal cancer is the third cancer cause of death in Spain. It is important to investigate new tumoral markers for early diagnosis, disease monitoring and prevention strategies. Telomeres protect the chromosome from degradation by nucleases and endto- end fusion. The progressive loss of the telomeric ends of chromosomes is an important mechanism in the timing of human cellular aging. Telomeric Repeat Factor 1 (TRF1) is a protein that binds at telomere ends. Purpose: to measure the concentrations of TRF1 and the relationships among telomere length, telomerase activity, and TRF1 levels in tumor and normal colorectal mucosa. Method: from normal and tumoral samples of 83 patients who underwent surgery for colorectal cancer we analyzed TRF1 protein concentration by Western Blot, telomerase activity, by the fluorescent- telomeric repeat amplification protocol assay and telomere length by Southern Blot. Results: high levels of TRF1 were observed in 68.7% of tumor samples, while the majority of normal samples (59%) showed negative or weak TRF1 concentrations. Among the tumor samples, telomere length was significantly associated with TRF1 protein levels (p = 0.023). Conclusions: a relationship was found between telomere length and TRF1 abundance protein in tumor samples, which means that TRF1 is an important factor in the tumor progression and maybe a diagnostic factor.Sociedad Española de Patología DigestivaArán Ediciones2012info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionhttps://doi.org/10.4321/S1130-01082012001000005http://hdl.handle.net/10459.1/49253reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a https://doi.org/10.4321/S1130-01082012001000005Revista Española de Enfermedades Digestivas, 2012, vol. 104, núm. 10, p. 530-536(c) Arán Ediciones, 2012info:eu-repo/semantics/openAccessoai:recercat.cat:10459.1/492532026-05-29T05:05:01Z
dc.title.none.fl_str_mv Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
title Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
spellingShingle Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
Valls i Bautista, Cristina
Telomeric repeat factor 1
Telomere length
Colorectal cancer
Telomerase activity
title_short Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
title_full Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
title_fullStr Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
title_full_unstemmed Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
title_sort Telomeric repeat factor 1 protein levels correlates with telomere length in colorectal cancer
dc.creator.none.fl_str_mv Valls i Bautista, Cristina
Piñol Felis, Carme
Reñé Espinet, Josep Maria
Buenestado Garcia, Juan
Viñas Salas, Joan
author Valls i Bautista, Cristina
author_facet Valls i Bautista, Cristina
Piñol Felis, Carme
Reñé Espinet, Josep Maria
Buenestado Garcia, Juan
Viñas Salas, Joan
author_role author
author2 Piñol Felis, Carme
Reñé Espinet, Josep Maria
Buenestado Garcia, Juan
Viñas Salas, Joan
author2_role author
author
author
author
dc.subject.none.fl_str_mv Telomeric repeat factor 1
Telomere length
Colorectal cancer
Telomerase activity
topic Telomeric repeat factor 1
Telomere length
Colorectal cancer
Telomerase activity
description Background: colorectal cancer is the third cancer cause of death in Spain. It is important to investigate new tumoral markers for early diagnosis, disease monitoring and prevention strategies. Telomeres protect the chromosome from degradation by nucleases and endto- end fusion. The progressive loss of the telomeric ends of chromosomes is an important mechanism in the timing of human cellular aging. Telomeric Repeat Factor 1 (TRF1) is a protein that binds at telomere ends. Purpose: to measure the concentrations of TRF1 and the relationships among telomere length, telomerase activity, and TRF1 levels in tumor and normal colorectal mucosa. Method: from normal and tumoral samples of 83 patients who underwent surgery for colorectal cancer we analyzed TRF1 protein concentration by Western Blot, telomerase activity, by the fluorescent- telomeric repeat amplification protocol assay and telomere length by Southern Blot. Results: high levels of TRF1 were observed in 68.7% of tumor samples, while the majority of normal samples (59%) showed negative or weak TRF1 concentrations. Among the tumor samples, telomere length was significantly associated with TRF1 protein levels (p = 0.023). Conclusions: a relationship was found between telomere length and TRF1 abundance protein in tumor samples, which means that TRF1 is an important factor in the tumor progression and maybe a diagnostic factor.
publishDate 2012
dc.date.none.fl_str_mv 2012
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://doi.org/10.4321/S1130-01082012001000005
http://hdl.handle.net/10459.1/49253
url https://doi.org/10.4321/S1130-01082012001000005
http://hdl.handle.net/10459.1/49253
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a https://doi.org/10.4321/S1130-01082012001000005
Revista Española de Enfermedades Digestivas, 2012, vol. 104, núm. 10, p. 530-536
dc.rights.none.fl_str_mv (c) Arán Ediciones, 2012
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Arán Ediciones, 2012
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Sociedad Española de Patología Digestiva
Arán Ediciones
publisher.none.fl_str_mv Sociedad Española de Patología Digestiva
Arán Ediciones
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411985275224064
score 15,223283

Similares en LA Referencia