Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease

Worldwide, Esca is a complex and devastating Grapevine Trunk Disease (GTD), characterized by inconstant foliar symptoms and internal wood degradation. A large range of fungal taxa have been reported as causal agents. We applied both culture-dependent and culture-independent methods (Illumina Technol...

ver descrição completa

Detalhes bibliográficos
Autores: Martín, Laura, García-García, Blanca, Alguacil García, María del Mar
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/410377
Acesso em linha:http://hdl.handle.net/10261/410377
https://api.elsevier.com/content/abstract/scopus_id/85143804367
Access Level:acceso abierto
Palavra-chave:Botryosphaeriaceae
GTDs
Hymenochaetaceae
Illumina® NGS
Phaeomoniella chlamydospora
Grapevine
Pathotrophs
id ES_801cb6bd5f7da6035244eeb1827eb11f
oai_identifier_str oai:digital.csic.es:10261/410377
network_acronym_str ES
network_name_str España
repository_id_str
spelling Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk DiseaseMartín, LauraGarcía-García, BlancaAlguacil García, María del MarBotryosphaeriaceaeGTDsHymenochaetaceaeIllumina® NGSPhaeomoniella chlamydosporaGrapevinePathotrophsWorldwide, Esca is a complex and devastating Grapevine Trunk Disease (GTD), characterized by inconstant foliar symptoms and internal wood degradation. A large range of fungal taxa have been reported as causal agents. We applied both culture-dependent and culture-independent methods (Illumina Technology and q-PCR) to investigate this concerning disease. Woods from vines with asymptomatic leaves and vines with leaf Esca symptoms were compared. Internally, different types of wood were found, from healthy wood with black necrosis to wood with white rot. A combination of leaf and wood Esca symptoms resulted in four experimental categories. Although there was no relation with symptoms, culture-independent mycobiome composition revealed Phaeomoniella chlamydospora, a GTD pathogen, as the most abundant species (detected in 85.4% of wood samples, with 14.8% relative abundance). Using TaqMan q-PCR, P. chlamydospora DNA was detected in 60.4% of samples (far from the 18.8% of positive results in the culture-dependent approach). There was a predominance of saprotrophs, even if their abundance was not affected by Esca symptoms. Concerning pathotrophs, the white rot development within grapevines was linked to the abundance of fungi belonging to the Hymenochaetaceae family. The Botryosphaeriaceae family was identified as an indicator for expression of Esca foliar symptoms. Lastly, the Aureobasidiaceae family was found to be a potential biocontrol agent for Esca, since it was most abundant in the control asymptomatic plantsThis work was financially supported by FEDER funds (“Una manera de hacer Europa”) and CICYTEX through MIPEX, IB18102 and GR21196 (Research Group AGA001) projects. L. Martín was supported by the DOC-INIA 2015 Program, and B. García-García received funding via the INIA-2019-0011 project to develop a PhD (PRE2019-087630)Peer reviewedMultidisciplinary Digital Publishing InstituteEuropean CommissionCSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)Alguacil García, María del Mar [0000-0001-8495-8707]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202520252022info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/410377https://api.elsevier.com/content/abstract/scopus_id/85143804367reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésInternational journal of molecular scienceshttps://doi.org/10.3390/ijms232314726Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4103772026-05-22T06:33:51Z
dc.title.none.fl_str_mv Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
title Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
spellingShingle Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
Martín, Laura
Botryosphaeriaceae
GTDs
Hymenochaetaceae
Illumina® NGS
Phaeomoniella chlamydospora
Grapevine
Pathotrophs
title_short Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
title_full Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
title_fullStr Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
title_full_unstemmed Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
title_sort Interactions of the Fungal Community in the Complex Patho-System of Esca, a Grapevine Trunk Disease
dc.creator.none.fl_str_mv Martín, Laura
García-García, Blanca
Alguacil García, María del Mar
author Martín, Laura
author_facet Martín, Laura
García-García, Blanca
Alguacil García, María del Mar
author_role author
author2 García-García, Blanca
Alguacil García, María del Mar
author2_role author
author
dc.contributor.none.fl_str_mv European Commission
CSIC - Instituto Nacional de Investigación y Tecnología Agraria y Alimentaria (INIA)
Alguacil García, María del Mar [0000-0001-8495-8707]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Botryosphaeriaceae
GTDs
Hymenochaetaceae
Illumina® NGS
Phaeomoniella chlamydospora
Grapevine
Pathotrophs
topic Botryosphaeriaceae
GTDs
Hymenochaetaceae
Illumina® NGS
Phaeomoniella chlamydospora
Grapevine
Pathotrophs
description Worldwide, Esca is a complex and devastating Grapevine Trunk Disease (GTD), characterized by inconstant foliar symptoms and internal wood degradation. A large range of fungal taxa have been reported as causal agents. We applied both culture-dependent and culture-independent methods (Illumina Technology and q-PCR) to investigate this concerning disease. Woods from vines with asymptomatic leaves and vines with leaf Esca symptoms were compared. Internally, different types of wood were found, from healthy wood with black necrosis to wood with white rot. A combination of leaf and wood Esca symptoms resulted in four experimental categories. Although there was no relation with symptoms, culture-independent mycobiome composition revealed Phaeomoniella chlamydospora, a GTD pathogen, as the most abundant species (detected in 85.4% of wood samples, with 14.8% relative abundance). Using TaqMan q-PCR, P. chlamydospora DNA was detected in 60.4% of samples (far from the 18.8% of positive results in the culture-dependent approach). There was a predominance of saprotrophs, even if their abundance was not affected by Esca symptoms. Concerning pathotrophs, the white rot development within grapevines was linked to the abundance of fungi belonging to the Hymenochaetaceae family. The Botryosphaeriaceae family was identified as an indicator for expression of Esca foliar symptoms. Lastly, the Aureobasidiaceae family was found to be a potential biocontrol agent for Esca, since it was most abundant in the control asymptomatic plants
publishDate 2022
dc.date.none.fl_str_mv 2022
2025
2025
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/410377
https://api.elsevier.com/content/abstract/scopus_id/85143804367
url http://hdl.handle.net/10261/410377
https://api.elsevier.com/content/abstract/scopus_id/85143804367
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv International journal of molecular sciences
https://doi.org/10.3390/ijms232314726
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869411874115682304
score 15,812455

Registos relacionados