Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals

Plants mainly acquire N from the soil in the form of nitrate (NO3-) or ammonium (NH4+). Ammonium-based nutrition is gaining interest because it helps to avoid the environmental pollution associated with nitrate fertilization. However, in general, plants prefer NO3- and indeed, when growing only with...

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Autores: De la Peña Cuao, Marlon, González Moro, María Begoña, Marino Bilbao, Daniel
Tipo de recurso: artículo
Fecha de publicación:2019
País:España
Institución:Universidad del País Vasco
Repositorio:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/40529
Acceso en línea:http://hdl.handle.net/10810/40529
Access Level:acceso abierto
Palabra clave:ammonium assimilation
Asn
carbon metabolism
Gln
monocots
nitrate
nitrogen metabolism
root
TCA cycle
glutamine-synthetase
phosphoenolpyruvate carboxylase
arabidopsis-thaliana
gene-expression
plant nitrogen
use efficiency
crucial role
tolerance
toxicity
metabolism
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spelling Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cerealsDe la Peña Cuao, MarlonGonzález Moro, María BegoñaMarino Bilbao, Danielammonium assimilationAsncarbon metabolismGlnmonocotsnitratenitrogen metabolismrootTCA cycleglutamine-synthetasephosphoenolpyruvate carboxylasearabidopsis-thalianagene-expressionplant nitrogenuse efficiencycrucial roletolerancetoxicitymetabolismPlants mainly acquire N from the soil in the form of nitrate (NO3-) or ammonium (NH4+). Ammonium-based nutrition is gaining interest because it helps to avoid the environmental pollution associated with nitrate fertilization. However, in general, plants prefer NO3- and indeed, when growing only with NH4+ they can encounter so-called ammonium stress. Since Brachypodium distachyon is a useful model species for the study of monocot physiology and genetics, we chose it to characterize performance under ammonium nutrition. Brachypodium distachyon Bd21 plants were grown hydroponically in 1 or 2.5 mM NO3- or NH4+. Nitrogen and carbon metabolism associated with NH4+ assimilation was evaluated in terms of tissue contents of NO3-, NH4+, K, Mg, Ca, amino acids and organic acids together with tricarboxylic acid (TCA) cycle and NH4+-assimilating enzyme activities and RNA transcript levels. The roots behaved as a physiological barrier preventing NH4+ translocation to aerial parts, as indicated by a sizeable accumulation of NH4+, Asn and Gln in the roots. A continuing high NH4+ assimilation rate was made possible by a tuning of the TCA cycle and its associated anaplerotic pathways to match 2-oxoglutarate and oxaloacetate demand for Gln and Asn synthesis. These results show B. distachyon to be a highly suitable tool for the study of the physiological, molecular and genetic basis of ammonium nutrition in cereals.This research was funded/supported by the Basque Government (IT932-16) and the Spanish Ministry of Economy and Competitiveness (BIO2017-84035-R co-funded by FEDER).Oxford University Press202020202019info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/40529reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/BIO2017-84035-R/https://academic.oup.com/aobpla/article/11/3/plz029/5487766info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited. (CC BY 4.0)Atribución 3.0 Españaoai:addi.ehu.eus:10810/405292026-06-18T09:23:17Z
dc.title.none.fl_str_mv Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
title Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
spellingShingle Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
De la Peña Cuao, Marlon
ammonium assimilation
Asn
carbon metabolism
Gln
monocots
nitrate
nitrogen metabolism
root
TCA cycle
glutamine-synthetase
phosphoenolpyruvate carboxylase
arabidopsis-thaliana
gene-expression
plant nitrogen
use efficiency
crucial role
tolerance
toxicity
metabolism
title_short Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
title_full Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
title_fullStr Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
title_full_unstemmed Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
title_sort Providing carbon skeletons to sustain amide synthesis in roots underlines the suitability of Brachypodium distachyon for the study of ammonium stress in cereals
dc.creator.none.fl_str_mv De la Peña Cuao, Marlon
González Moro, María Begoña
Marino Bilbao, Daniel
author De la Peña Cuao, Marlon
author_facet De la Peña Cuao, Marlon
González Moro, María Begoña
Marino Bilbao, Daniel
author_role author
author2 González Moro, María Begoña
Marino Bilbao, Daniel
author2_role author
author
dc.subject.none.fl_str_mv ammonium assimilation
Asn
carbon metabolism
Gln
monocots
nitrate
nitrogen metabolism
root
TCA cycle
glutamine-synthetase
phosphoenolpyruvate carboxylase
arabidopsis-thaliana
gene-expression
plant nitrogen
use efficiency
crucial role
tolerance
toxicity
metabolism
topic ammonium assimilation
Asn
carbon metabolism
Gln
monocots
nitrate
nitrogen metabolism
root
TCA cycle
glutamine-synthetase
phosphoenolpyruvate carboxylase
arabidopsis-thaliana
gene-expression
plant nitrogen
use efficiency
crucial role
tolerance
toxicity
metabolism
description Plants mainly acquire N from the soil in the form of nitrate (NO3-) or ammonium (NH4+). Ammonium-based nutrition is gaining interest because it helps to avoid the environmental pollution associated with nitrate fertilization. However, in general, plants prefer NO3- and indeed, when growing only with NH4+ they can encounter so-called ammonium stress. Since Brachypodium distachyon is a useful model species for the study of monocot physiology and genetics, we chose it to characterize performance under ammonium nutrition. Brachypodium distachyon Bd21 plants were grown hydroponically in 1 or 2.5 mM NO3- or NH4+. Nitrogen and carbon metabolism associated with NH4+ assimilation was evaluated in terms of tissue contents of NO3-, NH4+, K, Mg, Ca, amino acids and organic acids together with tricarboxylic acid (TCA) cycle and NH4+-assimilating enzyme activities and RNA transcript levels. The roots behaved as a physiological barrier preventing NH4+ translocation to aerial parts, as indicated by a sizeable accumulation of NH4+, Asn and Gln in the roots. A continuing high NH4+ assimilation rate was made possible by a tuning of the TCA cycle and its associated anaplerotic pathways to match 2-oxoglutarate and oxaloacetate demand for Gln and Asn synthesis. These results show B. distachyon to be a highly suitable tool for the study of the physiological, molecular and genetic basis of ammonium nutrition in cereals.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/40529
url http://hdl.handle.net/10810/40529
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/BIO2017-84035-R/
https://academic.oup.com/aobpla/article/11/3/plz029/5487766
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Oxford University Press
publisher.none.fl_str_mv Oxford University Press
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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