Human milk metals and metalloids shape infant microbiota

[EN] Background: The profile of metal(loid)s in human milk is essential for infant growth and development, yet its impact on the development of the infant microbiota remains unclear. Elements, such as manganese, zinc, iron or copper, play crucial roles in influencing infant health. Aim: To investiga...

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Detalles Bibliográficos
Autores: Flores Ventura, Eduard, Bernabeu, Manuel, Callejón-Leblic, Belén, Cabrera-Rubio, Raul, Yeruva, Laxmi, Estañ-Capell, Javier, Martinez-Costa, Cecilia, García-Barrera, Tamara, Collado, Maria Carmen
Tipo de recurso: artículo
Fecha de publicación:2024
País:España
Institución:Universitat Politècnica de València (UPV)
Repositorio:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
Idioma:inglés
OAI Identifier:oai:riunet.upv.es:10251/225672
Acceso en línea:https://riunet.upv.es/handle/10251/225672
Access Level:acceso abierto
Palabra clave:Human milk
Infant microbiota
Metals
Metallomic profiling
16S rRNA sequencing
Gut microbiota diversity
Early infant development
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dc.title.none.fl_str_mv Human milk metals and metalloids shape infant microbiota
title Human milk metals and metalloids shape infant microbiota
spellingShingle Human milk metals and metalloids shape infant microbiota
Flores Ventura, Eduard
Human milk
Infant microbiota
Metals
Metallomic profiling
16S rRNA sequencing
Gut microbiota diversity
Early infant development
title_short Human milk metals and metalloids shape infant microbiota
title_full Human milk metals and metalloids shape infant microbiota
title_fullStr Human milk metals and metalloids shape infant microbiota
title_full_unstemmed Human milk metals and metalloids shape infant microbiota
title_sort Human milk metals and metalloids shape infant microbiota
dc.creator.none.fl_str_mv Flores Ventura, Eduard
Bernabeu, Manuel
Callejón-Leblic, Belén
Cabrera-Rubio, Raul
Yeruva, Laxmi
Estañ-Capell, Javier
Martinez-Costa, Cecilia
García-Barrera, Tamara
Collado, Maria Carmen
author Flores Ventura, Eduard
author_facet Flores Ventura, Eduard
Bernabeu, Manuel
Callejón-Leblic, Belén
Cabrera-Rubio, Raul
Yeruva, Laxmi
Estañ-Capell, Javier
Martinez-Costa, Cecilia
García-Barrera, Tamara
Collado, Maria Carmen
author_role author
author2 Bernabeu, Manuel
Callejón-Leblic, Belén
Cabrera-Rubio, Raul
Yeruva, Laxmi
Estañ-Capell, Javier
Martinez-Costa, Cecilia
García-Barrera, Tamara
Collado, Maria Carmen
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv European Commission
Agencia Estatal de Investigación
European Regional Development Fund
Ministerio de Ciencia, Innovación y Universidades
Repositorio Institucional de la Universitat Politècnica de València Riunet
dc.subject.none.fl_str_mv Human milk
Infant microbiota
Metals
Metallomic profiling
16S rRNA sequencing
Gut microbiota diversity
Early infant development
topic Human milk
Infant microbiota
Metals
Metallomic profiling
16S rRNA sequencing
Gut microbiota diversity
Early infant development
description [EN] Background: The profile of metal(loid)s in human milk is essential for infant growth and development, yet its impact on the development of the infant microbiota remains unclear. Elements, such as manganese, zinc, iron or copper, play crucial roles in influencing infant health. Aim: To investigate the metal(loid) content within human milk and its influence on the infant's gut microbiota within the first 2 months after birth. Methods: Human milk samples and infant stool samples from 77 mother¿infant dyads in the MAMI cohort were collected at two time points: the early transitional stage and the mature stage. Metallomic profiling of human milk was conducted using inductively coupled plasma-mass spectrometry (ICP-MS). The infant gut microbiota was profiled through 16S rRNA amplicon sequencing and maternal¿infant clinical data were available. Spearman's rank correlation coefficientsprovided insights into metal(loid)¿microbiota relationships. Results: Independent cross-sectional analyses of mother¿infant pairs at two time points, significant variations in metal concentrations and differences in microbial abundances and diversities were observed. Notably, Bifidobacterium genus abundance was higher during the mature lactation stage. During early lactation, we found a significant positive correlation between infant gut Corynebacterium and human milk nickel concentrations, and negative correlations between Veillonella spp. and antimony, and Enterobacter spp. and copper. Additionally, Simpson's diversity was negatively correlated with iron. In the mature lactation stage, we identified eleven significant correlations between metals and microbiota. Notably, Klebsiella genus showed multiple negative correlations with iron, antimony, and vanadium. Conclusion: Our study highlights the significance of metal(loid)¿microbiota interactions in early infant development, indicating that infant gut Klebsiella genus may be particularly vulnerable to fluctuations in metal(loid) levels present in human milk, when compared to other genera. Future research should explore these interactions at a strain level and the implications on infant health and development. This trial was registered as NCT03552939.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024-12-01
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://riunet.upv.es/handle/10251/225672
url https://riunet.upv.es/handle/10251/225672
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-123073NB-C21 METALOMICA Y METODOLOGIAS (META)OMICAS PARA ESTUDIAR LA NEUROTOXICIDAD Y EFECTOS EN LA SALUD REPRODUCTIVA DE LOS METALES. INFLUENCIA DE LA MICROBIOTA INTESTINAL Y DEL SELENIO
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-139475OB-I00 EL IMPACTO DEL MICROBIOMA Y DIETA EN LA PROGRAMACION, CRECIMIENTO Y DESARROLLO DE LA SALUD INFANTIL: SEGUIMIENTO 6 AÑOS DE LA COHORTE MAMI
European Commission https://doi.org/10.13039/501100000780 H2020 639226 The Power of Maternal Microbes on Infant Health
Ministerio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 CEX2021-001189-S-20-1 Severo Ochoa
Agencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 JDC2022-050269-I
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
Reconocimiento - No comercial (by-nc)
http://creativecommons.org/licenses/by-nc/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv The Royal Society of Chemistry
publisher.none.fl_str_mv The Royal Society of Chemistry
dc.source.none.fl_str_mv reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
instname:Universitat Politècnica de València (UPV)
instname_str Universitat Politècnica de València (UPV)
reponame_str RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
collection RiuNet. Repositorio Institucional de la Universitat Politécnica de Valéncia
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Human milk metals and metalloids shape infant microbiotaFlores Ventura, EduardBernabeu, ManuelCallejón-Leblic, BelénCabrera-Rubio, RaulYeruva, LaxmiEstañ-Capell, JavierMartinez-Costa, CeciliaGarcía-Barrera, TamaraCollado, Maria CarmenHuman milkInfant microbiotaMetalsMetallomic profiling16S rRNA sequencingGut microbiota diversityEarly infant development[EN] Background: The profile of metal(loid)s in human milk is essential for infant growth and development, yet its impact on the development of the infant microbiota remains unclear. Elements, such as manganese, zinc, iron or copper, play crucial roles in influencing infant health. Aim: To investigate the metal(loid) content within human milk and its influence on the infant's gut microbiota within the first 2 months after birth. Methods: Human milk samples and infant stool samples from 77 mother¿infant dyads in the MAMI cohort were collected at two time points: the early transitional stage and the mature stage. Metallomic profiling of human milk was conducted using inductively coupled plasma-mass spectrometry (ICP-MS). The infant gut microbiota was profiled through 16S rRNA amplicon sequencing and maternal¿infant clinical data were available. Spearman's rank correlation coefficientsprovided insights into metal(loid)¿microbiota relationships. Results: Independent cross-sectional analyses of mother¿infant pairs at two time points, significant variations in metal concentrations and differences in microbial abundances and diversities were observed. Notably, Bifidobacterium genus abundance was higher during the mature lactation stage. During early lactation, we found a significant positive correlation between infant gut Corynebacterium and human milk nickel concentrations, and negative correlations between Veillonella spp. and antimony, and Enterobacter spp. and copper. Additionally, Simpson's diversity was negatively correlated with iron. In the mature lactation stage, we identified eleven significant correlations between metals and microbiota. Notably, Klebsiella genus showed multiple negative correlations with iron, antimony, and vanadium. Conclusion: Our study highlights the significance of metal(loid)¿microbiota interactions in early infant development, indicating that infant gut Klebsiella genus may be particularly vulnerable to fluctuations in metal(loid) levels present in human milk, when compared to other genera. Future research should explore these interactions at a strain level and the implications on infant health and development. This trial was registered as NCT03552939.The authors would like to thank the families involved in the MAMI study, as well as all the members of the MAMI cohort study. This research was supported by the European Research Council (ERC) under the European Union's Horizon 2020 Research and Innovation Programme (ERC starting grant no. 639226) and the Spanish Ministry of Science and Innovation (MAMI-Plus ref. PID2022-139475OB-I00); PID2021-123073NB-C21 from the Spanish Ministry of Science and Innovation (MICIN); the Generacion del Conocimiento (MCI/AEI/FEDER "Una manera de hacer Europa"), and UHU-1256905 and UHU-202009 from the FEDER Andalusian Operative Program 2014-2020 (Ministry of Economy, Knowledge, Business and Universities, Regional Government of Andalusia, Spain). E.F.V. is grateful for the predoctoral grant awarded by the Spanish Ministry of Science and Innovation and thanks the European Social Fund Plus (ESF+) for the training of doctors within the framework of the State Plan for Scientific, Technical and Innovation Research 2021-2023 (ref. CEX2021-001189-S-20-1). M.B. is grateful for the post-PhD grant "Juan de la Cierva" (ref. JDC2022-050269-I) supported by the Spanish government MCIU/AEI and the European Union "NextGenerationEU"/PRTR. R.C.R. acknowledges the support from the Generalitat-Valenciana for the grant of the Plan GenT project (CDEIGENT 2020). IATA-CSIC authors also acknowledge the Spanish government MCIN/AEI to the Center of Excellence Accreditation Severo Ochoa (CEX2021-001189-S/MCIN/AEI/10.13039/501100011033).The Royal Society of ChemistryEuropean CommissionAgencia Estatal de InvestigaciónEuropean Regional Development FundMinisterio de Ciencia, Innovación y UniversidadesRepositorio Institucional de la Universitat Politècnica de València Riunet20242024-12-01journal articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://riunet.upv.es/handle/10251/225672reponame:RiuNet. Repositorio Institucional de la Universitat Politécnica de Valénciainstname:Universitat Politècnica de València (UPV)InglésengAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2021-123073NB-C21 METALOMICA Y METODOLOGIAS (META)OMICAS PARA ESTUDIAR LA NEUROTOXICIDAD Y EFECTOS EN LA SALUD REPRODUCTIVA DE LOS METALES. INFLUENCIA DE LA MICROBIOTA INTESTINAL Y DEL SELENIOAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023 PID2022-139475OB-I00 EL IMPACTO DEL MICROBIOMA Y DIETA EN LA PROGRAMACION, CRECIMIENTO Y DESARROLLO DE LA SALUD INFANTIL: SEGUIMIENTO 6 AÑOS DE LA COHORTE MAMIEuropean Commission https://doi.org/10.13039/501100000780 H2020 639226 The Power of Maternal Microbes on Infant HealthMinisterio de Ciencia, Innovación y Universidades https://doi.org/10.13039/100014440 CEX2021-001189-S-20-1 Severo OchoaAgencia Estatal de Investigación http://dx.doi.org/10.13039/501100011033 JDC2022-050269-Iopen accesshttp://purl.org/coar/access_right/c_abf2Reconocimiento - No comercial (by-nc) http://creativecommons.org/licenses/by-nc/4.0/info:eu-repo/semantics/openAccessoai:riunet.upv.es:10251/2256722026-06-13T07:49:27Z
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