Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity

21 Pág.

Detalles Bibliográficos
Autores: Hurtado-Morillas, Clara, Martínez-Rodrigo, Abel, Orden, J. A., Urbina-Fuentes, Laura de, Mas, Alicia, Domínguez-Bernal, G.
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2024
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/357488
Acceso en línea:http://hdl.handle.net/10261/357488
https://api.elsevier.com/content/abstract/scopus_id/85186116856
Access Level:acceso abierto
Palabra clave:Leishmania infantum
LetiFend®
PLGA nanoparticles
Canine leishmaniosis
Multi-epitope peptide
Nanovaccine
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spelling Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell ImmunityHurtado-Morillas, ClaraMartínez-Rodrigo, AbelOrden, J. A.Urbina-Fuentes, Laura deMas, AliciaDomínguez-Bernal, G.Leishmania infantumLetiFend®PLGA nanoparticlesCanine leishmaniosisMulti-epitope peptideNanovaccine21 Pág.Canine leishmaniosis (CanL) is a growing health problem for which vaccination is a crucial tool for the control of disease. The successful development of an effective vaccine against this disease relies on eliciting a robust and enduring T-cell immune response involving the activation of CD4+ Th1 and CD8+ T-cells. This study aimed to evaluate the immunogenicity and prophylactic efficacy of a novel nanovaccine comprising a multi-epitope peptide, known as HisDTC, encapsulated in PLGA nanoparticles against Leishmania infantum infection in the murine model. The encapsulation strategy was designed to enhance antigen loading and sustain release, ensuring prolonged exposure to the immune system. Our results showed that mice immunized with PLGA-encapsulated HisDTC exhibited a significant reduction in the parasite load in the liver and spleen over both short and long-term duration. This reduction was associated with a cellular immune profile marked by elevated levels of pro-inflammatory cytokines, such as IFN-γ, and the generation of memory T cells. In conclusion, the current study establishes that PLGA-encapsulated HisDTC can promote effective and long-lasting T-cell responses against L. infantum in the murine model. These findings underscore the potential utility of multi-epitope vaccines, in conjunction with appropriate delivery systems, as an alternative strategy for CanL control.This research was partially supported by grants from the Spanish Government project PID2019-106487RB-I00/AEI/10.13039/501100011033, and from the Comunidad de Madrid (Spain) PLATESA2-CM (S2018/BAA-4370). Alicia Mas (A.M.) was supported by a postdoctoral fellowship (Margarita Salas) from European Union funds.Peer reviewedMultidisciplinary Digital Publishing InstituteAgencia Estatal de Investigación (España)Comunidad de MadridEuropean CommissionHurtado-Morillas, Clara [0000-0001-8041-4980]Martínez-Rodrigo, Abel [0000-0003-3405-7171]Orden, J. A. [0000-0001-6352-1426]Mas, Alicia [0000-0001-8947-3207]Domínguez-Bernal, G. [0000-0002-7693-653X]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202420242024info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/357488https://api.elsevier.com/content/abstract/scopus_id/85186116856reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106487RB-I00S2018/BAA-4370Centro de Investigación en Sanidad Animal (CISA)https://doi.org/10.3390/ani14040605Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3574882026-05-22T06:33:51Z
dc.title.none.fl_str_mv Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
spellingShingle Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
Hurtado-Morillas, Clara
Leishmania infantum
LetiFend®
PLGA nanoparticles
Canine leishmaniosis
Multi-epitope peptide
Nanovaccine
title_short Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_full Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_fullStr Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_full_unstemmed Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
title_sort Enhancing Control of Leishmania infantum Infection: A Multi-Epitope Nanovaccine for Durable T-Cell Immunity
dc.creator.none.fl_str_mv Hurtado-Morillas, Clara
Martínez-Rodrigo, Abel
Orden, J. A.
Urbina-Fuentes, Laura de
Mas, Alicia
Domínguez-Bernal, G.
author Hurtado-Morillas, Clara
author_facet Hurtado-Morillas, Clara
Martínez-Rodrigo, Abel
Orden, J. A.
Urbina-Fuentes, Laura de
Mas, Alicia
Domínguez-Bernal, G.
author_role author
author2 Martínez-Rodrigo, Abel
Orden, J. A.
Urbina-Fuentes, Laura de
Mas, Alicia
Domínguez-Bernal, G.
author2_role author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Comunidad de Madrid
European Commission
Hurtado-Morillas, Clara [0000-0001-8041-4980]
Martínez-Rodrigo, Abel [0000-0003-3405-7171]
Orden, J. A. [0000-0001-6352-1426]
Mas, Alicia [0000-0001-8947-3207]
Domínguez-Bernal, G. [0000-0002-7693-653X]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Leishmania infantum
LetiFend®
PLGA nanoparticles
Canine leishmaniosis
Multi-epitope peptide
Nanovaccine
topic Leishmania infantum
LetiFend®
PLGA nanoparticles
Canine leishmaniosis
Multi-epitope peptide
Nanovaccine
description 21 Pág.
publishDate 2024
dc.date.none.fl_str_mv 2024
2024
2024
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/357488
https://api.elsevier.com/content/abstract/scopus_id/85186116856
url http://hdl.handle.net/10261/357488
https://api.elsevier.com/content/abstract/scopus_id/85186116856
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-106487RB-I00
S2018/BAA-4370
Centro de Investigación en Sanidad Animal (CISA)
https://doi.org/10.3390/ani14040605
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
publisher.none.fl_str_mv Multidisciplinary Digital Publishing Institute
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,812455

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