Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana

Este estudio fue respaldado, en su totalidad o en parte, por la Fundación Bill y Melinda Gates (OPP1143172 e INV-005889).

Detalhes bibliográficos
Autores: Jiang, Xi, Coroian, Diana, Barahona, Emma, Echavarri-Erasun, Carlos, Castellanos-Rueda, Rocío, Eseverri, Álvaro, Aznar-Moreno, Jose, Buren, Stefan, Rubio, Luis Manuel
Formato: artículo
Fecha de publicación:2022
País:España
Recursos:Universidad Rey Juan Carlos
Repositorio:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
OAI Identifier:oai:burjcdigital.urjc.es:10115/27913
Acesso em linha:https://hdl.handle.net/10115/27913
Access Level:acceso abierto
Palavra-chave:FeMo-cofactor
metalloproteins
nif
nitrogen fixation
synthetic biology
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spelling Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamianaJiang, XiCoroian, DianaBarahona, EmmaEchavarri-Erasun, CarlosCastellanos-Rueda, RocíoEseverri, ÁlvaroAznar-Moreno, JoseBuren, StefanRubio, Luis ManuelFeMo-cofactormetalloproteinsnifnitrogen fixationsynthetic biologyEste estudio fue respaldado, en su totalidad o en parte, por la Fundación Bill y Melinda Gates (OPP1143172 e INV-005889).Engineering plants to synthesize nitrogenase and assimilate atmospheric N2 will reduce crop dependency on industrial N fertilizers. This technology can be achieved by expressing prokaryotic nitrogen fixation gene products for the assembly of a functional nitrogenase in plants. NifB is a critical nitrogenase component since it catalyzes the first committed step in the biosynthesis of all types of nitrogenase active-site cofactors. Here, we used a library of 30 distinct nifB sequences originating from different phyla and ecological niches to restore diazotrophic growth of an Azotobacter vinelandii nifB mutant. Twenty of these variants rescued the nifB mutant phenotype despite their phylogenetic distance to A. vinelandii. Because multiple protein interactions are required in the iron-molybdenum cofactor (FeMo-co) biosynthetic pathway, the maturation of nitrogenase in a heterologous host can be divided in independent modules containing interacting proteins that function together to produce a specific intermediate. Therefore, nifB functional modules composed of a nifB variant, together with the A. vinelandii NifS and NifU proteins (for biosynthesis of NifB [Fe4S4] clusters) and the FdxN ferredoxin (for NifB function), were expressed in Nicotiana benthamiana chloroplasts and mitochondria. Three archaeal NifB proteins accumulated at high levels in soluble fractions of chloroplasts (Methanosarcina acetivorans and Methanocaldococcus infernus) or mitochondria (M. infernus and Methanothermobacter thermautotrophicus). These NifB proteins were shown to accept [Fe4S4] clusters from NifU and were functional in FeMo-co synthesis in vitro. The accumulation of significant levels of soluble and functional NifB proteins in chloroplasts and mitochondria is critical to engineering biological nitrogen fixation in plants.American Society for Microbiology202320232022info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10115/27913reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlosinstname:Universidad Rey Juan CarlosInglésAtribución 4.0 Internacionalhttp://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:burjcdigital.urjc.es:10115/279132026-06-24T12:48:17Z
dc.title.none.fl_str_mv Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
title Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
spellingShingle Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
Jiang, Xi
FeMo-cofactor
metalloproteins
nif
nitrogen fixation
synthetic biology
title_short Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
title_full Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
title_fullStr Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
title_full_unstemmed Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
title_sort Functional nitrogenase cofactor maturase NifB in mitochondria and chloroplasts of Nicotiana benthamiana
dc.creator.none.fl_str_mv Jiang, Xi
Coroian, Diana
Barahona, Emma
Echavarri-Erasun, Carlos
Castellanos-Rueda, Rocío
Eseverri, Álvaro
Aznar-Moreno, Jose
Buren, Stefan
Rubio, Luis Manuel
author Jiang, Xi
author_facet Jiang, Xi
Coroian, Diana
Barahona, Emma
Echavarri-Erasun, Carlos
Castellanos-Rueda, Rocío
Eseverri, Álvaro
Aznar-Moreno, Jose
Buren, Stefan
Rubio, Luis Manuel
author_role author
author2 Coroian, Diana
Barahona, Emma
Echavarri-Erasun, Carlos
Castellanos-Rueda, Rocío
Eseverri, Álvaro
Aznar-Moreno, Jose
Buren, Stefan
Rubio, Luis Manuel
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv FeMo-cofactor
metalloproteins
nif
nitrogen fixation
synthetic biology
topic FeMo-cofactor
metalloproteins
nif
nitrogen fixation
synthetic biology
description Este estudio fue respaldado, en su totalidad o en parte, por la Fundación Bill y Melinda Gates (OPP1143172 e INV-005889).
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10115/27913
url https://hdl.handle.net/10115/27913
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv Atribución 4.0 Internacional
http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
instname:Universidad Rey Juan Carlos
instname_str Universidad Rey Juan Carlos
reponame_str BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
collection BURJC-Digital. Repositorio Institucional de la Universidad Rey Juan Carlos
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869410550002221056
score 15,812455

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