Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440

Purpose: Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when...

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Autores: López-Lara, L. I., Pazos-Rojas, L.A., López-Cruz, L. E., Morales-García, Y.E., Quintero-Hernández, V., Torre Zúñiga, Jesús de la, Van Dillewijn, Pieter
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2020
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/224923
Acceso en línea:http://hdl.handle.net/10261/224923
Access Level:acceso abierto
Palabra clave:Pazos-Rojas, L.A.
López-Cruz, L.E.
Morales-García, Y.E.
Quintero-Hernández
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spelling Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440López-Lara, L. I.Pazos-Rojas, L.A.López-Cruz, L. E.Morales-García, Y.E.Quintero-Hernández, V.Torre Zúñiga, Jesús de laVan Dillewijn, PieterPazos-Rojas, L.A.López-Cruz, L.E.Morales-García, Y.E.Quintero-HernándezPurpose: Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when desiccated cells are rehydrated for at least 24 h. Methods: To determine which genes of transport, oxidation-reduction, and transcription processes could be involved in the return of P. putida KT2440 to the culturable state, a transcriptome analysis was carried out comparing the gene expression of non-desiccated samples with samples subjected to desiccation followed by 20 min of rehydration or desiccation followed by 24 h of rehydration. Results: Desiccation stress triggered a VBNC state of P. putida. The major response was detected after 24 h of rehydration with 148 upregulated and 42 downregulated genes. During the VBNC state, P. putida activated transmembrane transport processes like that of siderophores through a TonB-dependent transporter and putative polyhydric alcohol transport systems. Prolonged rehydration with distilled water resuscitated P. putida KT2440 cells activating the catabolism of phenylalanine/tyrosine to provide energy and carbon for ubiquinone biosynthesis while maintaining a reduced protein synthesis. On the other hand, the interruption of the TonB-dependent receptor gene (PP_1446) increased desiccation survival of the mutant strain. Conclusion: The activation of the iron transport system (TonB-dependent siderophore receptor) and alcohol transport can be helping the VBNC state of P. putida. Activation of catabolism of phenylalanine/tyrosine and reduced protein synthesis was needed for resuscitation from the VBNC state.Funding was provided by the intramural program of the Vicerrectoria de Investigación y Estudios de Posgrado, BUAP through grants PV16-ID00450, PV17-ID00337. Lilia Isela Lopez Lara received a scholarship (number 273274) from the National Council of Science and Technology (CONACyT).Springer NatureBenemérita Universidad Autónoma de PueblaConsejo Nacional de Ciencia y Tecnología (México)Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020202020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/224923reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://link.springer.com/article/10.1186/s13213-020-01596-3Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2249232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
title Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
spellingShingle Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
López-Lara, L. I.
Pazos-Rojas, L.A.
López-Cruz, L.E.
Morales-García, Y.E.
Quintero-Hernández
title_short Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
title_full Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
title_fullStr Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
title_full_unstemmed Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
title_sort Influence of rehydration on transcriptome during resuscitation of desiccated Pseudomonas putida KT2440
dc.creator.none.fl_str_mv López-Lara, L. I.
Pazos-Rojas, L.A.
López-Cruz, L. E.
Morales-García, Y.E.
Quintero-Hernández, V.
Torre Zúñiga, Jesús de la
Van Dillewijn, Pieter
author López-Lara, L. I.
author_facet López-Lara, L. I.
Pazos-Rojas, L.A.
López-Cruz, L. E.
Morales-García, Y.E.
Quintero-Hernández, V.
Torre Zúñiga, Jesús de la
Van Dillewijn, Pieter
author_role author
author2 Pazos-Rojas, L.A.
López-Cruz, L. E.
Morales-García, Y.E.
Quintero-Hernández, V.
Torre Zúñiga, Jesús de la
Van Dillewijn, Pieter
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Benemérita Universidad Autónoma de Puebla
Consejo Nacional de Ciencia y Tecnología (México)
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Pazos-Rojas, L.A.
López-Cruz, L.E.
Morales-García, Y.E.
Quintero-Hernández
topic Pazos-Rojas, L.A.
López-Cruz, L.E.
Morales-García, Y.E.
Quintero-Hernández
description Purpose: Pseudomonas putida KT2440 is a desiccation-sensitive bacterium that loses culturability after 15 days of air desiccation. We have previously shown that P. putida KT2440 can develop a viable but nonculturable (VBNC) state after being exposed to desiccation stress and eventually recover when desiccated cells are rehydrated for at least 24 h. Methods: To determine which genes of transport, oxidation-reduction, and transcription processes could be involved in the return of P. putida KT2440 to the culturable state, a transcriptome analysis was carried out comparing the gene expression of non-desiccated samples with samples subjected to desiccation followed by 20 min of rehydration or desiccation followed by 24 h of rehydration. Results: Desiccation stress triggered a VBNC state of P. putida. The major response was detected after 24 h of rehydration with 148 upregulated and 42 downregulated genes. During the VBNC state, P. putida activated transmembrane transport processes like that of siderophores through a TonB-dependent transporter and putative polyhydric alcohol transport systems. Prolonged rehydration with distilled water resuscitated P. putida KT2440 cells activating the catabolism of phenylalanine/tyrosine to provide energy and carbon for ubiquinone biosynthesis while maintaining a reduced protein synthesis. On the other hand, the interruption of the TonB-dependent receptor gene (PP_1446) increased desiccation survival of the mutant strain. Conclusion: The activation of the iron transport system (TonB-dependent siderophore receptor) and alcohol transport can be helping the VBNC state of P. putida. Activation of catabolism of phenylalanine/tyrosine and reduced protein synthesis was needed for resuscitation from the VBNC state.
publishDate 2020
dc.date.none.fl_str_mv 2020
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/224923
url http://hdl.handle.net/10261/224923
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://link.springer.com/article/10.1186/s13213-020-01596-3
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Springer Nature
publisher.none.fl_str_mv Springer Nature
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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