Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )

Background: The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information abou...

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Autores: Coleto Reyes, Inmaculada, De la Peña Cuao, Marlon, Rodríguez Escalante, Jon, Bejarano Ayerbe, Iraide, Glauser, Gaetan, Aparicio Tejo, Pedro M., González Moro, María Begoña, Marino Bilbao, Daniel
Tipo de documento: artigo
Data de publicação:2017
País:España
Recursos:Universidad del País Vasco
Repositório:Addi. Archivo Digital para la Docencia y la Investigación
OAI Identifier:oai:addi.ehu.eus:10810/27270
Acesso em linha:http://hdl.handle.net/10810/27270
Access Level:Acceso aberto
Palavra-chave:ammonium
brassica napus
glucosinolates
nitrate
nitrogen
oilseed rape
sulfur
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spelling Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )Coleto Reyes, InmaculadaDe la Peña Cuao, MarlonRodríguez Escalante, JonBejarano Ayerbe, IraideGlauser, GaetanAparicio Tejo, Pedro M.González Moro, María BegoñaMarino Bilbao, Danielammoniumbrassica napusglucosinolatesnitratenitrogenoilseed rapesulfurBackground: The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM). Results: Metabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4+, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression. Conclusions: The results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.The design of the study, analysis and interpretation of data and writing of the manuscript was supported by the Basque Government [IT932-16], the Spanish Ministry of Economy and Competitiveness [AGL2015-68881-REDT] and [BIO2014-56271-R] co-funded by FEDER and the People Programme (Marie Curie Actions) of the European Union's Seventh Framework Programme (FP7/2007-2013) under REA grant agreement number 334019.Biomed Central201820182017info:eu-repo/semantics/articleapplication/pdfhttp://hdl.handle.net/10810/27270reponame:Addi. Archivo Digital para la Docencia y la Investigacióninstname:Universidad del País VascoInglésinfo:eu-repo/grantAgreement/MINECO/AGL2015-68881-REDT/info:eu-repo/grantAgreement/MINECO/BIO2014-56271-R/info:eu-repo/grantAgreement/EC/FP7/334019https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-017-1100-9info:eu-repo/semantics/openAccesshttp://creativecommons.org/licenses/by/3.0/es/This article is distributed under the terms of the Creative Commons Attribution 4.0 International License (http://creativecommons.org/licenses/by/4.0/), which permits unrestricted use, distribution, and reproduction in any medium, provided you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.Atribución 3.0 Españaoai:addi.ehu.eus:10810/272702026-06-18T09:23:17Z
dc.title.none.fl_str_mv Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
title Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
spellingShingle Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
Coleto Reyes, Inmaculada
ammonium
brassica napus
glucosinolates
nitrate
nitrogen
oilseed rape
sulfur
title_short Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
title_full Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
title_fullStr Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
title_full_unstemmed Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
title_sort Leaves play a central role in the adaptation of nitrogen and sulfur metabolism to ammonium nutrition in oilseed rape ( Brassica napus )
dc.creator.none.fl_str_mv Coleto Reyes, Inmaculada
De la Peña Cuao, Marlon
Rodríguez Escalante, Jon
Bejarano Ayerbe, Iraide
Glauser, Gaetan
Aparicio Tejo, Pedro M.
González Moro, María Begoña
Marino Bilbao, Daniel
author Coleto Reyes, Inmaculada
author_facet Coleto Reyes, Inmaculada
De la Peña Cuao, Marlon
Rodríguez Escalante, Jon
Bejarano Ayerbe, Iraide
Glauser, Gaetan
Aparicio Tejo, Pedro M.
González Moro, María Begoña
Marino Bilbao, Daniel
author_role author
author2 De la Peña Cuao, Marlon
Rodríguez Escalante, Jon
Bejarano Ayerbe, Iraide
Glauser, Gaetan
Aparicio Tejo, Pedro M.
González Moro, María Begoña
Marino Bilbao, Daniel
author2_role author
author
author
author
author
author
author
dc.subject.none.fl_str_mv ammonium
brassica napus
glucosinolates
nitrate
nitrogen
oilseed rape
sulfur
topic ammonium
brassica napus
glucosinolates
nitrate
nitrogen
oilseed rape
sulfur
description Background: The coordination between nitrogen (N) and sulfur (S) assimilation is required to suitably provide plants with organic compounds essential for their development and growth. The N source induces the adaptation of many metabolic processes in plants; however, there is scarce information about the influence that it may exert on the functioning of S metabolism. The aim of this work was to provide an overview of N and S metabolism in oilseed rape (Brassica napus) when exposed to different N sources. To do so, plants were grown in hydroponic conditions with nitrate or ammonium as N source at two concentrations (0.5 and 1 mM). Results: Metabolic changes mainly occurred in leaves, where ammonium caused the up-regulation of enzymes involved in the primary assimilation of N and a general increase in the concentration of N-compounds (NH4+, amino acids and proteins). Similarly, the activity of key enzymes of primary S assimilation and the content of S-compounds (glutathione and glucosinolates) were also higher in leaves of ammonium-fed plants. Interestingly, sulfate level was lower in leaves of ammonium-fed plants, which was accompanied by the down-regulation of SULTR1 transporters gene expression. Conclusions: The results highlight the impact of the N source on different steps of N and S metabolism in oilseed rape, notably inducing N and S assimilation in leaves, and put forward the potential of N source management to modulate the synthesis of compounds with biotechnological interest, such as glucosinolates.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10810/27270
url http://hdl.handle.net/10810/27270
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv info:eu-repo/grantAgreement/MINECO/AGL2015-68881-REDT/
info:eu-repo/grantAgreement/MINECO/BIO2014-56271-R/
info:eu-repo/grantAgreement/EC/FP7/334019
https://bmcplantbiol.biomedcentral.com/articles/10.1186/s12870-017-1100-9
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
eu_rights_str_mv openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/3.0/es/
Atribución 3.0 España
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Biomed Central
publisher.none.fl_str_mv Biomed Central
dc.source.none.fl_str_mv reponame:Addi. Archivo Digital para la Docencia y la Investigación
instname:Universidad del País Vasco
instname_str Universidad del País Vasco
reponame_str Addi. Archivo Digital para la Docencia y la Investigación
collection Addi. Archivo Digital para la Docencia y la Investigación
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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