Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides

Sensitivities to polycationic peptides and EDTA were compared in Yersinia enterocolitica pathogenic and environmental biogroups. As shown by changes in permeability to the fluorescent hydrophobic probe N-phenylnaphthylamine (NPN), the outer membranes (OMs) of pathogenic and environmental strains gro...

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Autores: Bengoechea, J.A. (José A.)|||/items/200f73db-0871-416d-90a7-79d648267682, Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c, Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
Tipo de recurso: artículo
Fecha de publicación:1996
País:España
Institución:Universidad de Navarra
Repositorio:Dadun. Depósito Académico Digital de la Universidad de Navarra
Idioma:inglés
OAI Identifier:oai:dadun.unav.edu:10171/29431
Acceso en línea:https://hdl.handle.net/10171/29431
Access Level:acceso abierto
Palabra clave:Bacterial outer membrane proteins metabolism
Edetic acid pharmacology
Peptides pharmacology
Yersinia enterocolitica metabolism
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spelling Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptidesBengoechea, J.A. (José A.)|||/items/200f73db-0871-416d-90a7-79d648267682Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861cMoriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7bBacterial outer membrane proteins metabolismEdetic acid pharmacologyPeptides pharmacologyYersinia enterocolitica metabolismSensitivities to polycationic peptides and EDTA were compared in Yersinia enterocolitica pathogenic and environmental biogroups. As shown by changes in permeability to the fluorescent hydrophobic probe N-phenylnaphthylamine (NPN), the outer membranes (OMs) of pathogenic and environmental strains grown at 26 degrees C in standard broth were more resistant to poly-L-lysine, poly-L-ornithine, melittin, cecropin P1, polymyxin B, and EDTA than Escherichia coli OMs. At 37 degrees C, OMs of pathogenic biogroups were resistant to EDTA and polycations and OMs of environmental strains were resistant to EDTA whereas E. coli OMs were sensitive to both EDTA and polycations. Similar results were found when testing deoxycholate sensitivity after polycation exposure or when isogenic pairs with or without virulence plasmid pYV were compared. With bacteria grown without Ca++ available, OM permeability to NPN was drastically increased in pathogenic but not in environmental strains or E. coli. Under these conditions, OMs of pYV+ and pYV- cells showed small differences in NPN permeability but differences in polycation sensitivity could not be detected by fluorimetry. O:1,6 (environmental type) lipopolysaccharide (LPS), but not O:3 or O:8 LPS, was markedly rough at 37 degrees C, and this could explain the differences in polycation sensitivity. LPSs from serotypes O:3 and O:8 grown at 37 degrees C were more permeable to NPN than O:1,6 LPS, and O:8 LPS was resistant to polycation-induced permeabilization. These data suggest that LPSs relate to some but not all the OM differences described. It is hypothesized that the different OM properties of environmental and pathogenic biogroups reflect the adaptation of the latter biogroups to pathogenicity.American Society for MicrobiologyDadun. Depósito Académico Digital Universidad de Navarra20132013-06-2519961996-01-0119961996-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/29431reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/294312026-06-21T12:47:57Z
dc.title.none.fl_str_mv Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
title Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
spellingShingle Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
Bengoechea, J.A. (José A.)|||/items/200f73db-0871-416d-90a7-79d648267682
Bacterial outer membrane proteins metabolism
Edetic acid pharmacology
Peptides pharmacology
Yersinia enterocolitica metabolism
title_short Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
title_full Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
title_fullStr Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
title_full_unstemmed Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
title_sort Outer membrane differences between pathogenic and environmental Yersinia enterocolitica biogroups probed with hydrophobic permeants and polycationic peptides
dc.creator.none.fl_str_mv Bengoechea, J.A. (José A.)|||/items/200f73db-0871-416d-90a7-79d648267682
Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
author Bengoechea, J.A. (José A.)|||/items/200f73db-0871-416d-90a7-79d648267682
author_facet Bengoechea, J.A. (José A.)|||/items/200f73db-0871-416d-90a7-79d648267682
Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
author_role author
author2 Diaz, R. (Ramón)|||/items/5326d716-1718-4b1a-adf2-64bb72a0861c
Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b
author2_role author
author
dc.contributor.none.fl_str_mv Dadun. Depósito Académico Digital Universidad de Navarra
dc.subject.none.fl_str_mv Bacterial outer membrane proteins metabolism
Edetic acid pharmacology
Peptides pharmacology
Yersinia enterocolitica metabolism
topic Bacterial outer membrane proteins metabolism
Edetic acid pharmacology
Peptides pharmacology
Yersinia enterocolitica metabolism
description Sensitivities to polycationic peptides and EDTA were compared in Yersinia enterocolitica pathogenic and environmental biogroups. As shown by changes in permeability to the fluorescent hydrophobic probe N-phenylnaphthylamine (NPN), the outer membranes (OMs) of pathogenic and environmental strains grown at 26 degrees C in standard broth were more resistant to poly-L-lysine, poly-L-ornithine, melittin, cecropin P1, polymyxin B, and EDTA than Escherichia coli OMs. At 37 degrees C, OMs of pathogenic biogroups were resistant to EDTA and polycations and OMs of environmental strains were resistant to EDTA whereas E. coli OMs were sensitive to both EDTA and polycations. Similar results were found when testing deoxycholate sensitivity after polycation exposure or when isogenic pairs with or without virulence plasmid pYV were compared. With bacteria grown without Ca++ available, OM permeability to NPN was drastically increased in pathogenic but not in environmental strains or E. coli. Under these conditions, OMs of pYV+ and pYV- cells showed small differences in NPN permeability but differences in polycation sensitivity could not be detected by fluorimetry. O:1,6 (environmental type) lipopolysaccharide (LPS), but not O:3 or O:8 LPS, was markedly rough at 37 degrees C, and this could explain the differences in polycation sensitivity. LPSs from serotypes O:3 and O:8 grown at 37 degrees C were more permeable to NPN than O:1,6 LPS, and O:8 LPS was resistant to polycation-induced permeabilization. These data suggest that LPSs relate to some but not all the OM differences described. It is hypothesized that the different OM properties of environmental and pathogenic biogroups reflect the adaptation of the latter biogroups to pathogenicity.
publishDate 1996
dc.date.none.fl_str_mv 1996
1996-01-01
1996
1996-01-01
2013
2013-06-25
dc.type.none.fl_str_mv journal article
http://purl.org/coar/resource_type/c_6501
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://hdl.handle.net/10171/29431
url https://hdl.handle.net/10171/29431
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv American Society for Microbiology
publisher.none.fl_str_mv American Society for Microbiology
dc.source.none.fl_str_mv reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra
instname:Universidad de Navarra
instname_str Universidad de Navarra
reponame_str Dadun. Depósito Académico Digital de la Universidad de Navarra
collection Dadun. Depósito Académico Digital de la Universidad de Navarra
repository.name.fl_str_mv
repository.mail.fl_str_mv
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