Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa

[Data availability] All data supporting the findings of this study are available within the paper and the Supplementary Information. RNA-Seq data can be retrieved from the NCBI Sequence Read Archive (SRA) [https://www.ncbi.nlm.nih.gov/sra] under the BioProject accession ID PRJNA1246127.

Detalhes bibliográficos
Autores: Maestro-Gaitan, Isaac, González-Bodi, S., Hassinger-Lino, Gabriel Rennato, Redondo-Nieto, Miguel, Rodríguez-Casillas, Laura, Matías, Javier, Zamarreño, Ángel M., García-Mina, José María, Granado Rodríguez, Sara, Bolaños, Luis, Reguera, María
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2025
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/417186
Acesso em linha:http://hdl.handle.net/10261/417186
https://api.elsevier.com/content/abstract/scopus_id/105025397110
Access Level:Acceso aberto
Palavra-chave:Abiotic stress
Development
Drought tolerance
Metabolism
Quinoa
Source-sink relationships
id ES_683a6dbfc04e022e903d863cedc4a3b3
oai_identifier_str oai:digital.csic.es:10261/417186
network_acronym_str ES
network_name_str España
repository_id_str
dc.title.none.fl_str_mv Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
title Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
spellingShingle Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
Maestro-Gaitan, Isaac
Abiotic stress
Development
Drought tolerance
Metabolism
Quinoa
Source-sink relationships
title_short Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
title_full Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
title_fullStr Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
title_full_unstemmed Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
title_sort Transcriptional reprogramming under drought reveals divergent adaptive strategies in Quinoa
dc.creator.none.fl_str_mv Maestro-Gaitan, Isaac
González-Bodi, S.
Hassinger-Lino, Gabriel Rennato
Redondo-Nieto, Miguel
Rodríguez-Casillas, Laura
Matías, Javier
Zamarreño, Ángel M.
García-Mina, José María
Granado Rodríguez, Sara
Bolaños, Luis
Reguera, María
author Maestro-Gaitan, Isaac
author_facet Maestro-Gaitan, Isaac
González-Bodi, S.
Hassinger-Lino, Gabriel Rennato
Redondo-Nieto, Miguel
Rodríguez-Casillas, Laura
Matías, Javier
Zamarreño, Ángel M.
García-Mina, José María
Granado Rodríguez, Sara
Bolaños, Luis
Reguera, María
author_role author
author2 González-Bodi, S.
Hassinger-Lino, Gabriel Rennato
Redondo-Nieto, Miguel
Rodríguez-Casillas, Laura
Matías, Javier
Zamarreño, Ángel M.
García-Mina, José María
Granado Rodríguez, Sara
Bolaños, Luis
Reguera, María
author2_role author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agencia Estatal de Investigación (España)
Ministerio de Ciencia e Innovación (España)
European Commission
González-Bodi, S. [0000-0003-3178-2976]
Granado Rodríguez, Sara [0000-0001-9618-5051]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Abiotic stress
Development
Drought tolerance
Metabolism
Quinoa
Source-sink relationships
topic Abiotic stress
Development
Drought tolerance
Metabolism
Quinoa
Source-sink relationships
description [Data availability] All data supporting the findings of this study are available within the paper and the Supplementary Information. RNA-Seq data can be retrieved from the NCBI Sequence Read Archive (SRA) [https://www.ncbi.nlm.nih.gov/sra] under the BioProject accession ID PRJNA1246127.
publishDate 2025
dc.date.none.fl_str_mv 2025
2026
2026
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/417186
https://api.elsevier.com/content/abstract/scopus_id/105025397110
url http://hdl.handle.net/10261/417186
https://api.elsevier.com/content/abstract/scopus_id/105025397110
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/AEI//CEX2020-000999-S-20-4
info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137688OB-I00
The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1186/s12870-025-07687-0
https://doi.org/10.1186/s12870-025-07687-0
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Springer Nature
BioMed Central
publisher.none.fl_str_mv Springer Nature
BioMed Central
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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spelling Transcriptional reprogramming under drought reveals divergent adaptive strategies in QuinoaMaestro-Gaitan, IsaacGonzález-Bodi, S.Hassinger-Lino, Gabriel RennatoRedondo-Nieto, MiguelRodríguez-Casillas, LauraMatías, JavierZamarreño, Ángel M.García-Mina, José MaríaGranado Rodríguez, SaraBolaños, LuisReguera, MaríaAbiotic stressDevelopmentDrought toleranceMetabolismQuinoaSource-sink relationships[Data availability] All data supporting the findings of this study are available within the paper and the Supplementary Information. RNA-Seq data can be retrieved from the NCBI Sequence Read Archive (SRA) [https://www.ncbi.nlm.nih.gov/sra] under the BioProject accession ID PRJNA1246127.[Background] Quinoa (Chenopodium quinoa Willd.) is known for its resilience to drought, yet the organ-specific transcriptional mechanisms underlying this trait remain insufficiently characterized. To address this, we investigated long-term drought responses in two contrasting cultivars, a sensitive (F15) and a tolerant (F16) genotype, by analyzing gene expression in leaves and seeds across three key reproductive stages: early (milky), thick, and mature seed development. This study complements previous physiological and biochemical investigations in the same genotypes, offering a deeper understanding of the molecular basis of drought tolerance.[Results] Transcriptomic profiling has revealed distinct drought responses based on cultivar and organ. The sensitive cultivar F15 exhibited rapid changes in gene expression, with significant downregulation of photosynthesis- and ribosome-related genes, suggesting growth arrest. In contrast, F16 showed more stable expression patterns and maintained essential metabolic functions longer under stress. Regulation of genes involved in sugar metabolism, raffinose oligosaccharides, phenylpropanoids, and fatty acids varied between the two genotypes and tissues. Hormonal profiling indicated that F16 leaves had reduced jasmonate levels and increased cytokinin accumulation, indicating sustained growth potential. In F15 leaves, salicylic acid (SA) levels increased during the early stages. Additionally, mature F16 seeds showed reduced jasmonoyl-isoleucine levels, reflecting organ-specific and genotype-dependent hormonal changes.[Conclusions] These results suggest that drought tolerance in quinoa involves a finely regulated, genotype-specific coordination of gene networks and hormonal pathways that control development, metabolism, and stress responses in an organ-dependent manner. The tolerant cultivar F16 exhibited a more controlled transcriptional and hormonal response under drought, maintaining photosynthetic and metabolic stability while activating protective mechanisms related to sugar and osmolyte metabolism, including raffinose and stachyose biosynthesis, as well as cytokinin-mediated regulation of senescence. This study identifies molecular targets for future breeding efforts to improve quinoa drought tolerance for cultivation in water-limited environments.The authors gratefully acknowledge the financial support received from the Ministerio de Ciencia e Innovación (MICINN, Spain) and the Agencia Estatal de Investigación (MCIN/AEI/https://doi.org/10.13039/501100011033) through the grants PID2022-137688OB-I00 and CNS2022-135167. Research was also supported by grants from the Spanish Government/CEX2020-000999-S-20-4 Severo Ochoa Program for Centres of Excellence to CBGP, funded by MCIN/AEI/https://doi.org/10.13039/501100011033 (to LRC).With funding form the Spanish government through the "Severa Ochoa Centre of Excellence" accreditation (CEX2020-000999-S-20-4).Peer reviewedSpringer NatureBioMed CentralAgencia Estatal de Investigación (España)Ministerio de Ciencia e Innovación (España)European CommissionGonzález-Bodi, S. [0000-0003-3178-2976]Granado Rodríguez, Sara [0000-0001-9618-5051]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202620262025info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/417186https://api.elsevier.com/content/abstract/scopus_id/105025397110reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/AEI//CEX2020-000999-S-20-4info:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2021-2023/PID2022-137688OB-I00The underlying dataset has been published as supplementary material of the article in the publisher platform at DOI https://doi.org/10.1186/s12870-025-07687-0https://doi.org/10.1186/s12870-025-07687-0Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/4171862026-05-22T06:33:51Z
score 15,812455

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