Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles

To evaluate the effects of dietary protein/carbohydrate (P/CHO) ratio and feeding frequency (FF) on growth, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles, two practical isolipidic (17%) diets were formulated to include high protein (50%)/ low starch...

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Authors: Basto-Silva, Catarina, Enes, Paula, Oliva-Teles, Aires, Capilla Campos, Encarnación, Guerreiro, Inês
Format: article
Status:Versión aceptada para publicación
Publication Date:2022
Country:España
Institution:Universidad de Barcelona
Repository:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/186265
Online Access:https://hdl.handle.net/2445/186265
Access Level:Open access
Keyword:Expressió gènica
Orada
Gene expression
Sparus aurata
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repository_id_str
spelling Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juvenilesBasto-Silva, CatarinaEnes, PaulaOliva-Teles, AiresCapilla Campos, EncarnaciónGuerreiro, InêsExpressió gènicaOradaGene expressionSparus aurataTo evaluate the effects of dietary protein/carbohydrate (P/CHO) ratio and feeding frequency (FF) on growth, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles, two practical isolipidic (17%) diets were formulated to include high protein (50%)/ low starch (10%) (diet P50/CHO10) or low protein (40%)/ high starch (20%) (diet P40/CHO20). Triplicate groups of fish with 9.1 ± 0.01 g were fed for 60 days with these diets until visual satiation at three FF: one (9:00), two (9:00 and 17:00), or three (9:00, 13:00, and 17:00) meals per day. Dietary P/CHO ratios did not affect growth performance while feeding 2 or 3 meals per day improved fish growth. Fish fed diet P40/CHO20 had increased feed intake (FI), protein efficiency ratio (PER), and nitrogen retention (NR), and lower feed efficiency (FE), nitrogen intake (NI), and economic conversion ratio (ECR). Feeding 2 or 3 meals per day increased FI, NI, ECR, and economic profit index, and decreased FE, PER, and NR. Fish fed diet P40/CHO20 presented increased hepatic lipid and glycogen content, hepatocyte area covered by lipid vacuoles, and glucokinase (gk) gene expression, and decreased glutamate dehydrogenase expression. Fish fed 3 meals per day had decreased plasma triglycerides and total protein levels, while fish fed 2 or 3 meals per day presented decreased hepatic growth hormone receptor-i (ghr-i), gk, and fatty acid synthase gene expression. Interaction between P/CHO ratio and FF was only observed in plasmatic glucose, cholesterol, and total lipids levels, and insulin-like growth factor-1, and ghr-ii gene expression. Overall, glycogenesis, glycolysis, and economic efficiency seemed to be increased while the amino acid catabolism was reduced in fish fed the P40/CHO20 diet. Higher FF increased growth and economic efficiency, and reduced glycolysis and lipogenesis pathways. In conclusion, a diet with P40/CHO20 ratio fed twice a day appears to be the most adequate strategy regarding feed utilization and economic efficiency for gilthead seabream juveniles in order to achieve optimum sustainable aquaculture.Elsevier B.V.2022info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/186265Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2022.738182Aquaculture, 2022, vol. 554, p. 738182https://doi.org/10.1016/j.aquaculture.2022.738182cc-by-nc-nd (c) Elsevier B.V., 2022https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1862652026-05-27T06:46:51Z
dc.title.none.fl_str_mv Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
title Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
spellingShingle Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
Basto-Silva, Catarina
Expressió gènica
Orada
Gene expression
Sparus aurata
title_short Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
title_full Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
title_fullStr Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
title_full_unstemmed Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
title_sort Dietary protein/carbohydrate ratio and feeding frequency affect feed utilization, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles
dc.creator.none.fl_str_mv Basto-Silva, Catarina
Enes, Paula
Oliva-Teles, Aires
Capilla Campos, Encarnación
Guerreiro, Inês
author Basto-Silva, Catarina
author_facet Basto-Silva, Catarina
Enes, Paula
Oliva-Teles, Aires
Capilla Campos, Encarnación
Guerreiro, Inês
author_role author
author2 Enes, Paula
Oliva-Teles, Aires
Capilla Campos, Encarnación
Guerreiro, Inês
author2_role author
author
author
author
dc.subject.none.fl_str_mv Expressió gènica
Orada
Gene expression
Sparus aurata
topic Expressió gènica
Orada
Gene expression
Sparus aurata
description To evaluate the effects of dietary protein/carbohydrate (P/CHO) ratio and feeding frequency (FF) on growth, intermediary metabolism, and economic efficiency of gilthead seabream (Sparus aurata) juveniles, two practical isolipidic (17%) diets were formulated to include high protein (50%)/ low starch (10%) (diet P50/CHO10) or low protein (40%)/ high starch (20%) (diet P40/CHO20). Triplicate groups of fish with 9.1 ± 0.01 g were fed for 60 days with these diets until visual satiation at three FF: one (9:00), two (9:00 and 17:00), or three (9:00, 13:00, and 17:00) meals per day. Dietary P/CHO ratios did not affect growth performance while feeding 2 or 3 meals per day improved fish growth. Fish fed diet P40/CHO20 had increased feed intake (FI), protein efficiency ratio (PER), and nitrogen retention (NR), and lower feed efficiency (FE), nitrogen intake (NI), and economic conversion ratio (ECR). Feeding 2 or 3 meals per day increased FI, NI, ECR, and economic profit index, and decreased FE, PER, and NR. Fish fed diet P40/CHO20 presented increased hepatic lipid and glycogen content, hepatocyte area covered by lipid vacuoles, and glucokinase (gk) gene expression, and decreased glutamate dehydrogenase expression. Fish fed 3 meals per day had decreased plasma triglycerides and total protein levels, while fish fed 2 or 3 meals per day presented decreased hepatic growth hormone receptor-i (ghr-i), gk, and fatty acid synthase gene expression. Interaction between P/CHO ratio and FF was only observed in plasmatic glucose, cholesterol, and total lipids levels, and insulin-like growth factor-1, and ghr-ii gene expression. Overall, glycogenesis, glycolysis, and economic efficiency seemed to be increased while the amino acid catabolism was reduced in fish fed the P40/CHO20 diet. Higher FF increased growth and economic efficiency, and reduced glycolysis and lipogenesis pathways. In conclusion, a diet with P40/CHO20 ratio fed twice a day appears to be the most adequate strategy regarding feed utilization and economic efficiency for gilthead seabream juveniles in order to achieve optimum sustainable aquaculture.
publishDate 2022
dc.date.none.fl_str_mv 2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/186265
url https://hdl.handle.net/2445/186265
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1016/j.aquaculture.2022.738182
Aquaculture, 2022, vol. 554, p. 738182
https://doi.org/10.1016/j.aquaculture.2022.738182
dc.rights.none.fl_str_mv cc-by-nc-nd (c) Elsevier B.V., 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc-by-nc-nd (c) Elsevier B.V., 2022
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier B.V.
publisher.none.fl_str_mv Elsevier B.V.
dc.source.none.fl_str_mv Articles publicats en revistes (Biologia Cel·lular, Fisiologia i Immunologia)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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