Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor

This work presents the scale-up of the conidia production of the entomopathogenic fungus Beauveria bassiana using two different wastes, coupled with concentration and virulence tests of the produced conidia against the pest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state ferm...

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Autores: Sala Martí, Arnau|||0000-0001-7010-6984, Barrena, Raquel|||0000-0002-6077-7765, Meyling, Nicolai Vitt, Artola, Adriana|||0000-0002-0524-2119
Tipo de recurso: artículo
Fecha de publicación:2023
País:España
Institución:Universitat Autònoma de Barcelona
Repositorio:Dipòsit Digital de Documents de la UAB
Idioma:inglés
OAI Identifier:oai:ddd.uab.cat:274808
Acceso en línea:https://ddd.uab.cat/record/274808
https://dx.doi.org/urn:doi:10.1016/j.jenvman.2023.118059
Access Level:acceso abierto
Palabra clave:Solid state fermentation
Substrate biodegradability
Virulence
Beauveria bassiana
Tenebrio molitor
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spelling Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactorEffect of substrate biodegradability on conidia virulenceSala Martí, Arnau|||0000-0001-7010-6984Barrena, Raquel|||0000-0002-6077-7765Meyling, Nicolai VittArtola, Adriana|||0000-0002-0524-2119Solid state fermentationSubstrate biodegradabilityVirulenceBeauveria bassianaTenebrio molitorThis work presents the scale-up of the conidia production of the entomopathogenic fungus Beauveria bassiana using two different wastes, coupled with concentration and virulence tests of the produced conidia against the pest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state fermentation (SSF) operating under batch strategy. Two substrates with different biodegradability (rice husk and beer draff) were tested, successfully scaling from 1.5 L to 22 L bioreactors. Higher conidia production was reached using beer draff as substrate (2.5 × 109 and 6.0 × 108 conidia g-1 dry matter in 1.5 and 22 L reactors respectively) highlighting air free porosity relevance as scale-up parameter. Concentration and dose-response tests against larvae and adult Tenebrio molitor were performed to compare strain CECT 20374 with control strain KVL 13-39 (a B. bassiana strain previously tested against T. molitor). Virulence effect of the 22 L fermentation product of strain CECT using rice husk or beer draff was tested against T. molitor adult stage. However, quality loses between conidia produced in agar plates and fermented products were observed (from 75 to 80% mortality in plates to 40% in rice husk and 50-60% in beer draff fermented products respectively). The differences between plate and fermented samples also indicated fermentation process, extraction and conservation steps as possible causes for quality losses, highlighting the need to optimize them to maximize virulence maintenance. 22023-01-0120232023-01-01Articlehttp://purl.org/coar/resource_type/c_6501VoRhttp://purl.org/coar/version/c_970fb48d4fbd8a85info:eu-repo/semantics/articleapplication/pdfhttps://ddd.uab.cat/record/274808https://dx.doi.org/urn:doi:10.1016/j.jenvman.2023.118059reponame:Dipòsit Digital de Documents de la UABinstname:Universitat Autònoma de BarcelonaInglésengAgencia Estatal de Investigación https://doi.org/10.13039/501100011033 PLEC2022-009252open accesshttp://purl.org/coar/access_right/c_abf2Aquest document està subjecte a una llicència d'ús Creative Commons. Es permet la reproducció total o parcial, la distribució, i la comunicació pública de l'obra, sempre que no sigui amb finalitats comercials, i sempre que es reconegui l'autoria de l'obra original. No es permet la creació d'obres derivades.https://creativecommons.org/licenses/by-nc-nd/4.0/info:eu-repo/semantics/openAccessoai:ddd.uab.cat:2748082026-06-06T12:50:31Z
dc.title.none.fl_str_mv Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
Effect of substrate biodegradability on conidia virulence
title Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
spellingShingle Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
Sala Martí, Arnau|||0000-0001-7010-6984
Solid state fermentation
Substrate biodegradability
Virulence
Beauveria bassiana
Tenebrio molitor
title_short Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
title_full Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
title_fullStr Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
title_full_unstemmed Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
title_sort Conidia production of the entomopathogenic fungus Beauveria bassiana using packed-bed bioreactor
dc.creator.none.fl_str_mv Sala Martí, Arnau|||0000-0001-7010-6984
Barrena, Raquel|||0000-0002-6077-7765
Meyling, Nicolai Vitt
Artola, Adriana|||0000-0002-0524-2119
author Sala Martí, Arnau|||0000-0001-7010-6984
author_facet Sala Martí, Arnau|||0000-0001-7010-6984
Barrena, Raquel|||0000-0002-6077-7765
Meyling, Nicolai Vitt
Artola, Adriana|||0000-0002-0524-2119
author_role author
author2 Barrena, Raquel|||0000-0002-6077-7765
Meyling, Nicolai Vitt
Artola, Adriana|||0000-0002-0524-2119
author2_role author
author
author
dc.subject.none.fl_str_mv Solid state fermentation
Substrate biodegradability
Virulence
Beauveria bassiana
Tenebrio molitor
topic Solid state fermentation
Substrate biodegradability
Virulence
Beauveria bassiana
Tenebrio molitor
description This work presents the scale-up of the conidia production of the entomopathogenic fungus Beauveria bassiana using two different wastes, coupled with concentration and virulence tests of the produced conidia against the pest Tenebrio molitor. Beauveria bassiana CECT 20374 was used in solid state fermentation (SSF) operating under batch strategy. Two substrates with different biodegradability (rice husk and beer draff) were tested, successfully scaling from 1.5 L to 22 L bioreactors. Higher conidia production was reached using beer draff as substrate (2.5 × 109 and 6.0 × 108 conidia g-1 dry matter in 1.5 and 22 L reactors respectively) highlighting air free porosity relevance as scale-up parameter. Concentration and dose-response tests against larvae and adult Tenebrio molitor were performed to compare strain CECT 20374 with control strain KVL 13-39 (a B. bassiana strain previously tested against T. molitor). Virulence effect of the 22 L fermentation product of strain CECT using rice husk or beer draff was tested against T. molitor adult stage. However, quality loses between conidia produced in agar plates and fermented products were observed (from 75 to 80% mortality in plates to 40% in rice husk and 50-60% in beer draff fermented products respectively). The differences between plate and fermented samples also indicated fermentation process, extraction and conservation steps as possible causes for quality losses, highlighting the need to optimize them to maximize virulence maintenance.
publishDate 2023
dc.date.none.fl_str_mv 2
2023-01-01
2023
2023-01-01
dc.type.none.fl_str_mv Article
http://purl.org/coar/resource_type/c_6501
VoR
http://purl.org/coar/version/c_970fb48d4fbd8a85
dc.type.openaire.fl_str_mv info:eu-repo/semantics/article
format article
dc.identifier.none.fl_str_mv https://ddd.uab.cat/record/274808
https://dx.doi.org/urn:doi:10.1016/j.jenvman.2023.118059
url https://ddd.uab.cat/record/274808
https://dx.doi.org/urn:doi:10.1016/j.jenvman.2023.118059
dc.language.none.fl_str_mv Inglés
eng
language_invalid_str_mv Inglés
language eng
dc.relation.none.fl_str_mv Agencia Estatal de Investigación https://doi.org/10.13039/501100011033 PLEC2022-009252
dc.rights.none.fl_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
dc.rights.openaire.fl_str_mv info:eu-repo/semantics/openAccess
rights_invalid_str_mv open access
http://purl.org/coar/access_right/c_abf2
https://creativecommons.org/licenses/by-nc-nd/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.source.none.fl_str_mv reponame:Dipòsit Digital de Documents de la UAB
instname:Universitat Autònoma de Barcelona
instname_str Universitat Autònoma de Barcelona
reponame_str Dipòsit Digital de Documents de la UAB
collection Dipòsit Digital de Documents de la UAB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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