Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis

Chagas disease is caused by the parasite Trypanosoma cruzi. It affects several million people, mainly in Latin America, and severe cardiac and/or digestive complications occur in ~30% of the chronically infected patients. Disease acute stage is mostly asymptomatic and infection goes undiagnosed. In...

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Detalhes bibliográficos
Autores: Egüez, Karina E., Alonso Padilla, Julio, Teran, Carolina, Chipana, Zenobia, García, Wilson, Torrico, Faustino, Gascón i Brustenga, Joaquim, Lozano Beltran, Daniel-Franz, Pinazo, Maria-Jesus
Tipo de documento: artigo
Estado:Versão publicada
Data de publicação:2017
País:España
Recursos:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositório:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/110395
Acesso em linha:https://hdl.handle.net/2445/110395
Access Level:Acceso aberto
Palavra-chave:Malaltia de Chagas
Diagnòstic
Chagas' disease
Diagnosis
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spelling Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosisEgüez, Karina E.Alonso Padilla, JulioTeran, CarolinaChipana, ZenobiaGarcía, WilsonTorrico, FaustinoGascón i Brustenga, JoaquimLozano Beltran, Daniel-FranzPinazo, Maria-JesusMalaltia de ChagasDiagnòsticChagas' diseaseDiagnosisChagas disease is caused by the parasite Trypanosoma cruzi. It affects several million people, mainly in Latin America, and severe cardiac and/or digestive complications occur in ~30% of the chronically infected patients. Disease acute stage is mostly asymptomatic and infection goes undiagnosed. In the chronic phase direct parasite detection is hampered due to its concealed presence and diagnosis is achieved by serological methods, like ELISA or indirect hemagglutination assays. Agreement in at least two tests must be obtained due to parasite wide antigenic variability. These techniques require equipped labs and trained personnel and are not available in distant regions. As a result, many infected people often remain undiagnosed until it is too late, as the two available chemotherapies show diminished efficacy in the advanced chronic stage. Easy-to-use rapid diagnostic tests have been developed to be implemented in remote areas as an alternative to conventional tests. They do not need electricity, nor cold chain, they can return results within an hour and some even work with whole blood as sample, like Chagas Stat-Pak (ChemBio Inc.) and Chagas Detect Plus (InBIOS Inc.). Nonetheless, in order to qualify a rapidly diagnosed positive patient for treatment, conventional serological confirmation is obligatory, which might risk its start. In this study two rapid tests based on distinct antigen sets were used in parallel as a way to obtain a fast and conclusive Chagas disease diagnosis using whole blood samples. Chagas Stat-Pak and Chagas Detect Plus were validated by comparison with three conventional tests yielding 100% sensitivity and 99.3% specificity over 342 patients seeking Chagas disease diagnosis in a reference centre in Sucre (Bolivia). Combined used of RDTs in distant regions could substitute laborious conventional serology, allowing immediate treatment and favouring better adhesion to it.Public Library of Science (PLoS)2017201720172017info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersion11 p.application/pdfhttps://hdl.handle.net/2445/110395Articles publicats en revistes (ISGlobal)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0005501PLoS Neglected Tropical Diseases, 2017, vol. 11, num. 4, p. e0005501http://dx.doi.org/10.1371/journal.pntd.0005501cc by (c) Egüez et al., 2017http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1103952026-05-29T05:05:01Z
dc.title.none.fl_str_mv Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
title Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
spellingShingle Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
Egüez, Karina E.
Malaltia de Chagas
Diagnòstic
Chagas' disease
Diagnosis
title_short Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
title_full Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
title_fullStr Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
title_full_unstemmed Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
title_sort Rapid diagnostic tests duo as alternative to conventional serological assays for conclusive Chagas disease diagnosis
dc.creator.none.fl_str_mv Egüez, Karina E.
Alonso Padilla, Julio
Teran, Carolina
Chipana, Zenobia
García, Wilson
Torrico, Faustino
Gascón i Brustenga, Joaquim
Lozano Beltran, Daniel-Franz
Pinazo, Maria-Jesus
author Egüez, Karina E.
author_facet Egüez, Karina E.
Alonso Padilla, Julio
Teran, Carolina
Chipana, Zenobia
García, Wilson
Torrico, Faustino
Gascón i Brustenga, Joaquim
Lozano Beltran, Daniel-Franz
Pinazo, Maria-Jesus
author_role author
author2 Alonso Padilla, Julio
Teran, Carolina
Chipana, Zenobia
García, Wilson
Torrico, Faustino
Gascón i Brustenga, Joaquim
Lozano Beltran, Daniel-Franz
Pinazo, Maria-Jesus
author2_role author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Malaltia de Chagas
Diagnòstic
Chagas' disease
Diagnosis
topic Malaltia de Chagas
Diagnòstic
Chagas' disease
Diagnosis
description Chagas disease is caused by the parasite Trypanosoma cruzi. It affects several million people, mainly in Latin America, and severe cardiac and/or digestive complications occur in ~30% of the chronically infected patients. Disease acute stage is mostly asymptomatic and infection goes undiagnosed. In the chronic phase direct parasite detection is hampered due to its concealed presence and diagnosis is achieved by serological methods, like ELISA or indirect hemagglutination assays. Agreement in at least two tests must be obtained due to parasite wide antigenic variability. These techniques require equipped labs and trained personnel and are not available in distant regions. As a result, many infected people often remain undiagnosed until it is too late, as the two available chemotherapies show diminished efficacy in the advanced chronic stage. Easy-to-use rapid diagnostic tests have been developed to be implemented in remote areas as an alternative to conventional tests. They do not need electricity, nor cold chain, they can return results within an hour and some even work with whole blood as sample, like Chagas Stat-Pak (ChemBio Inc.) and Chagas Detect Plus (InBIOS Inc.). Nonetheless, in order to qualify a rapidly diagnosed positive patient for treatment, conventional serological confirmation is obligatory, which might risk its start. In this study two rapid tests based on distinct antigen sets were used in parallel as a way to obtain a fast and conclusive Chagas disease diagnosis using whole blood samples. Chagas Stat-Pak and Chagas Detect Plus were validated by comparison with three conventional tests yielding 100% sensitivity and 99.3% specificity over 342 patients seeking Chagas disease diagnosis in a reference centre in Sucre (Bolivia). Combined used of RDTs in distant regions could substitute laborious conventional serology, allowing immediate treatment and favouring better adhesion to it.
publishDate 2017
dc.date.none.fl_str_mv 2017
2017
2017
2017
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/110395
url https://hdl.handle.net/2445/110395
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1371/journal.pntd.0005501
PLoS Neglected Tropical Diseases, 2017, vol. 11, num. 4, p. e0005501
http://dx.doi.org/10.1371/journal.pntd.0005501
dc.rights.none.fl_str_mv cc by (c) Egüez et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv cc by (c) Egüez et al., 2017
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 11 p.
application/pdf
dc.publisher.none.fl_str_mv Public Library of Science (PLoS)
publisher.none.fl_str_mv Public Library of Science (PLoS)
dc.source.none.fl_str_mv Articles publicats en revistes (ISGlobal)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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