Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species

Aging is characterized by a number of hallmarks including loss of mitochondrial homeostasis and decay in stress tolerance, among others. Unicellular eukaryotes have been widely used to study chronological aging. As a general trait, calorie restriction and activation of mitochondrial respiration has...

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Authors: Gröger, Anna, Martínez-Albo, Ilune, Albà Soler, Mar, Ayté del Olmo, José, Vega, Montserrat, Hidalgo Hernando, Elena
Format: article
Status:Published version
Publication Date:2023
Country:España
Institution:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repository:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:10230/58515
Online Access:http://hdl.handle.net/10230/58515
http://dx.doi.org/10.3390/antiox12101810
Access Level:Open access
Keyword:Ascomycota
H2O2 tolerance
S. cerevisiae
S. pombe
Aging
Mitochondrial activity
Respiration
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spelling Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast speciesGröger, AnnaMartínez-Albo, IluneAlbà Soler, MarAyté del Olmo, JoséVega, MontserratHidalgo Hernando, ElenaAscomycotaH2O2 toleranceS. cerevisiaeS. pombeAgingMitochondrial activityRespirationAging is characterized by a number of hallmarks including loss of mitochondrial homeostasis and decay in stress tolerance, among others. Unicellular eukaryotes have been widely used to study chronological aging. As a general trait, calorie restriction and activation of mitochondrial respiration has been proposed to contribute to an elongated lifespan. Most aging-related studies have been conducted with the Crabtree-positive yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, and with deletion collections deriving from these conventional yeast models. We have performed an unbiased characterization of longevity using thirteen fungi species, including S. cerevisiae and S. pombe, covering a wide range of the Ascomycota clade. We have determined their mitochondrial activity by oxygen consumption, complex IV activity, and mitochondrial redox potential, and the results derived from these three methodologies are highly overlapping. We have phenotypically compared the lifespans of the thirteen species and their capacity to tolerate oxidative stress. Longevity and elevated tolerance to hydrogen peroxide are correlated in some but not all yeasts. Mitochondrial activity per se cannot anticipate the length of the lifespan. We have classified the strains in four groups, with members of group 1 (Kluyveromyces lactis, Saccharomyces bayanus and Lodderomyces elongisporus) displaying high mitochondrial activity, elevated resistance to oxidative stress, and elongated lifespan.This work is supported by grants PGC2018-093920-B-I00 and PID2021-122837NB-I00 funded by MICIN/AEI/10.13039/501100011033/FEDER, UE to E.H., funded by Ministerio de Ciencia e Innovación, Agencia Estatal de Investigación and Fondo Europeo de Desarrollo Regional. The Oxidative Stress and Cell Cycle group is also supported by Generalitat de Catalunya (Spain) (2017-SGR-539 and 2021-SGR-00007) and by Excellence Unit María de Maeztu Grant CEX2018-000792-M funded by MCIN/AEI/10.13039/501100011033. E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain).MDPI202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfapplication/pdfhttp://hdl.handle.net/10230/58515http://dx.doi.org/10.3390/antiox12101810reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésAntioxidants (Basel). 2023 Sep 28;12(10):1810info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00info:eu-repo/grantAgreement/ES/3PE/PID2021-122837NB-I00info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M© 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).http://creativecommons.org/licenses/by/4.0/info:eu-repo/semantics/openAccessoai:recercat.cat:10230/585152026-05-29T05:05:01Z
dc.title.none.fl_str_mv Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
title Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
spellingShingle Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
Gröger, Anna
Ascomycota
H2O2 tolerance
S. cerevisiae
S. pombe
Aging
Mitochondrial activity
Respiration
title_short Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
title_full Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
title_fullStr Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
title_full_unstemmed Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
title_sort Comparing mitochondrial activity, oxidative stress tolerance, and longevity of thirteen Ascomycota yeast species
dc.creator.none.fl_str_mv Gröger, Anna
Martínez-Albo, Ilune
Albà Soler, Mar
Ayté del Olmo, José
Vega, Montserrat
Hidalgo Hernando, Elena
author Gröger, Anna
author_facet Gröger, Anna
Martínez-Albo, Ilune
Albà Soler, Mar
Ayté del Olmo, José
Vega, Montserrat
Hidalgo Hernando, Elena
author_role author
author2 Martínez-Albo, Ilune
Albà Soler, Mar
Ayté del Olmo, José
Vega, Montserrat
Hidalgo Hernando, Elena
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Ascomycota
H2O2 tolerance
S. cerevisiae
S. pombe
Aging
Mitochondrial activity
Respiration
topic Ascomycota
H2O2 tolerance
S. cerevisiae
S. pombe
Aging
Mitochondrial activity
Respiration
description Aging is characterized by a number of hallmarks including loss of mitochondrial homeostasis and decay in stress tolerance, among others. Unicellular eukaryotes have been widely used to study chronological aging. As a general trait, calorie restriction and activation of mitochondrial respiration has been proposed to contribute to an elongated lifespan. Most aging-related studies have been conducted with the Crabtree-positive yeasts Saccharomyces cerevisiae and Schizosaccharomyces pombe, and with deletion collections deriving from these conventional yeast models. We have performed an unbiased characterization of longevity using thirteen fungi species, including S. cerevisiae and S. pombe, covering a wide range of the Ascomycota clade. We have determined their mitochondrial activity by oxygen consumption, complex IV activity, and mitochondrial redox potential, and the results derived from these three methodologies are highly overlapping. We have phenotypically compared the lifespans of the thirteen species and their capacity to tolerate oxidative stress. Longevity and elevated tolerance to hydrogen peroxide are correlated in some but not all yeasts. Mitochondrial activity per se cannot anticipate the length of the lifespan. We have classified the strains in four groups, with members of group 1 (Kluyveromyces lactis, Saccharomyces bayanus and Lodderomyces elongisporus) displaying high mitochondrial activity, elevated resistance to oxidative stress, and elongated lifespan.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10230/58515
http://dx.doi.org/10.3390/antiox12101810
url http://hdl.handle.net/10230/58515
http://dx.doi.org/10.3390/antiox12101810
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Antioxidants (Basel). 2023 Sep 28;12(10):1810
info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00
info:eu-repo/grantAgreement/ES/3PE/PID2021-122837NB-I00
info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
dc.rights.none.fl_str_mv http://creativecommons.org/licenses/by/4.0/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv http://creativecommons.org/licenses/by/4.0/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
application/pdf
dc.publisher.none.fl_str_mv MDPI
publisher.none.fl_str_mv MDPI
dc.source.none.fl_str_mv reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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