Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles

We present a comprehensive study of two indocarbocyanines, DiI (1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate) and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate), in water suspensions in the presence of a CTAB surfactant both above and below the critic...

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Autores: Delledonne, Andrea, Morla Folch, Judit, Anzola, Mattia, Bertocchi, Francesco, Vargas Nadal, Guillem, Köber, Mariana, Sissa, Cristina, Ventosa, Nora, Painelli, Anna
Tipo de recurso: artículo
Estado:Versión publicada
Fecha de publicación:2021
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/263644
Acceso en línea:http://hdl.handle.net/10261/263644
https://api.elsevier.com/content/abstract/scopus_id/85114029447
Access Level:acceso abierto
Palabra clave:Cyanine dyes
Fluorescence properties
Single-molecule
Nanoparticles
Scattering
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dc.title.none.fl_str_mv Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
title Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
spellingShingle Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
Delledonne, Andrea
Cyanine dyes
Fluorescence properties
Single-molecule
Nanoparticles
Scattering
title_short Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
title_full Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
title_fullStr Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
title_full_unstemmed Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
title_sort Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micelles
dc.creator.none.fl_str_mv Delledonne, Andrea
Morla Folch, Judit
Anzola, Mattia
Bertocchi, Francesco
Vargas Nadal, Guillem
Köber, Mariana
Sissa, Cristina
Ventosa, Nora
Painelli, Anna
author Delledonne, Andrea
author_facet Delledonne, Andrea
Morla Folch, Judit
Anzola, Mattia
Bertocchi, Francesco
Vargas Nadal, Guillem
Köber, Mariana
Sissa, Cristina
Ventosa, Nora
Painelli, Anna
author_role author
author2 Morla Folch, Judit
Anzola, Mattia
Bertocchi, Francesco
Vargas Nadal, Guillem
Köber, Mariana
Sissa, Cristina
Ventosa, Nora
Painelli, Anna
author2_role author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministero dell'Istruzione, dell'Università e della Ricerca
Ministerio de Economía y Competitividad (España)
Ministerio de Ciencia, Innovación y Universidades (España)
European Commission
Generalitat de Catalunya
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cyanine dyes
Fluorescence properties
Single-molecule
Nanoparticles
Scattering
topic Cyanine dyes
Fluorescence properties
Single-molecule
Nanoparticles
Scattering
description We present a comprehensive study of two indocarbocyanines, DiI (1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate) and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate), in water suspensions in the presence of a CTAB surfactant both above and below the critical micellar concentration. The very good affinity of the two dyes with CTAB allows them to be brought into aqueous solutions minimizing aggregation phenomena. When the dyes are loaded inside the micelles, stable fluorescent nanostructures are formed that can be exploited for fundamental studies and for imaging applications. Of special interest are micelles loaded with both dyes: indeed, the large local dye concentration inside the micelles allows observing resonance energy transfer in systems where the global dye concentration is maintained at a low level, so that detailed and robust spectroscopic characterization is possible. Quite impressively, the efficiency of resonance energy transfer is boosted when diluting the sample below the critical micellar concentration. This counterintuitive result is explained in terms of the very large affinity between the dyes and CTAB which favors the dynamical formation of small molecular clusters containing both dyes. Fluorescent micelles are widely used in bioimaging and pharmacokinetic applications. More specifically, the observation of resonant energy transfer in micelles or more generally in nanostructures loaded with two dyes is routinely exploited as a way to assess the nanostructure integrity. In this context, our results demonstrate the importance of robust spectroscopic characterization of the relevant systems in different environments in order to safely assess the viability of the integrity test of nanoparticles based on resonant energy transfer.
publishDate 2021
dc.date.none.fl_str_mv 2021
2022
2022
dc.type.none.fl_str_mv info:eu-repo/semantics/article
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Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/263644
https://api.elsevier.com/content/abstract/scopus_id/85114029447
url http://hdl.handle.net/10261/263644
https://api.elsevier.com/content/abstract/scopus_id/85114029447
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
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info:eu-repo/grantAgreement/MICIU/Plan Estatal de investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000917-S
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Journal of Materials Chemistry C
http://dx.doi.org/10.1039/d1tc02888j

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Royal Society of Chemistry (UK)
publisher.none.fl_str_mv Royal Society of Chemistry (UK)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
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spelling Increasing resonance energy transfer upon dilution: A counterintuitive observation in CTAB micellesDelledonne, AndreaMorla Folch, JuditAnzola, MattiaBertocchi, FrancescoVargas Nadal, GuillemKöber, MarianaSissa, CristinaVentosa, NoraPainelli, AnnaCyanine dyesFluorescence propertiesSingle-moleculeNanoparticlesScatteringWe present a comprehensive study of two indocarbocyanines, DiI (1,1′-dioctadecyl-3,3,3′3′-tetramethylindocarbocyanine perchlorate) and DiD (1,1′-dioctadecyl-3,3,3′,3′-tetramethylindodicarbocyanine perchlorate), in water suspensions in the presence of a CTAB surfactant both above and below the critical micellar concentration. The very good affinity of the two dyes with CTAB allows them to be brought into aqueous solutions minimizing aggregation phenomena. When the dyes are loaded inside the micelles, stable fluorescent nanostructures are formed that can be exploited for fundamental studies and for imaging applications. Of special interest are micelles loaded with both dyes: indeed, the large local dye concentration inside the micelles allows observing resonance energy transfer in systems where the global dye concentration is maintained at a low level, so that detailed and robust spectroscopic characterization is possible. Quite impressively, the efficiency of resonance energy transfer is boosted when diluting the sample below the critical micellar concentration. This counterintuitive result is explained in terms of the very large affinity between the dyes and CTAB which favors the dynamical formation of small molecular clusters containing both dyes. Fluorescent micelles are widely used in bioimaging and pharmacokinetic applications. More specifically, the observation of resonant energy transfer in micelles or more generally in nanostructures loaded with two dyes is routinely exploited as a way to assess the nanostructure integrity. In this context, our results demonstrate the importance of robust spectroscopic characterization of the relevant systems in different environments in order to safely assess the viability of the integrity test of nanoparticles based on resonant energy transfer.This work is dedicated to Concepció Rovira and Jaume Veciana, inspiring scientists in the field of functional molecular materials. Working with them is a prized privilege and the basis for a solid friendship. AD, MA, FB, CS and AP benefited from the equipment and support of the COMP-HUB Initiative, funded by the “Departments of Excellence” program of the Italian Ministry for Education, University and Research (MIUR, 2018–2022) and acknowledge the support from the high performance computing center at Parma University. JMF, GVN, MK and NV acknowledge support from MINECO through the Severo Ochoa Programme FUNFUTURE (SEV-2015-0496 and CEX2019-000917-S), and the Ministry of Science and Innovation of Spain through the grant PID2019-105622RB-I00 (Mol4Bio), and gratefully acknowledge the financial support received from European Union's Horizon 2020 research and innovation program under the Marie Skłodowska-Curie grant agreement No. 712949 (TECNIOspring PLUS) and from the Agency for Business Competitiveness of the Government of Catalonia.With funding from the Spanish government through the ‘Severo Ochoa Centre of Excellence’ accreditation (CEX2019-000917-S).Peer reviewedRoyal Society of Chemistry (UK)Ministero dell'Istruzione, dell'Università e della RicercaMinisterio de Economía y Competitividad (España)Ministerio de Ciencia, Innovación y Universidades (España)European CommissionGeneralitat de CatalunyaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202220222021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/263644https://api.elsevier.com/content/abstract/scopus_id/85114029447reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MICIU/Plan Estatal de investigación Científica y Técnica y de Innovación 2017-2020/CEX2019-000917-Sinfo:eu-repo/grantAgreement/AEI/Plan Estatal de Investigación Científica y Técnica y de Innovación 2017-2020/PID2019-105622RB-I00info:eu-repo/grantAgreement/EC/H2020/712949Journal of Materials Chemistry Chttp://dx.doi.org/10.1039/d1tc02888jSíinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2636442026-05-22T06:33:51Z
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