Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics

129 p.-7 fig.-7 fig.supl.-1 tab.supl.

Detalles Bibliográficos
Autores: Frattini, Camilla, Villa, Sara, Pellicanò, Grazia, Jossen, Rachel, Katou, Yuki, Shirahige, Katsuhiko, Bermejo, Rodrigo
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2017
País:España
Institución:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/160840
Acceso en línea:http://hdl.handle.net/10261/160840
Access Level:acceso abierto
Palabra clave:Cdc148/p97
DNA replication
Checkpoint
Chromosome dynamics
Cohesins
Genome integrity
Replication forks
Replication stress
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spelling Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamicsFrattini, CamillaVilla, SaraPellicanò, GraziaJossen, RachelKatou, YukiShirahige, KatsuhikoBermejo, RodrigoCdc148/p97DNA replicationCheckpointChromosome dynamicsCohesinsGenome integrityReplication forksReplication stress129 p.-7 fig.-7 fig.supl.-1 tab.supl.Replication fork integrity is challenged in conditions of stress and protected by the Mec1/ATR checkpoint to preserve genome stability. Still poorly understood in fork protection is the role played by the structural maintenance of chromosomes (SMC) cohesin complex. We uncovered a role for the Rsp5Bul2 ubiquitin ligase in promoting survival to replication stress by preserving stalled fork integrity. Rsp5Bul2 physically interacts with cohesin and the Mec1 kinase, thus promoting checkpoint-dependent cohesin ubiquitylation and cohesin-mediated fork protection. Ubiquitylation mediated by Rsp5Bul2 promotes cohesin mobilization from chromatin neighboring stalled forks, likely by stimulating the Cdc48/p97 ubiquitin-selective segregase, and its timely association to nascent chromatids. This Rsp5Bul2 fork protection mechanism requires the Wpl1 cohesin mobilizer as well as the function of the Eco1 acetyltransferase securing sister chromatid entrapment. Our data indicate that ubiquitylation facilitates cohesin dynamic interfacing with replication forks within a mechanism preserving stalled-fork functional architecture.This work was supported by the Spanish Ministry of Science and Innovation [RYC-2010-07131 and BFU2011-24909 to R.B.], the Ministry of Economy and Competitiveness [BFU2014-52529-R to R.B.]), the European Community’s 7th Framework Programme [under grant agreement nº 293770 to R.B.], the Spanish National Research Council (CSIC) [PII-.201410I001], the Castilla y León regional government [to C.F.], the Spanish Formación del Personal Investigador (FPI) program [to S.V-H. and G.P.] and JSPS KAKENHI (15H05976 and 15H02369) [to K.S.].Peer reviewedElsevierMinisterio de Ciencia e Innovación (España)Ministerio de Economía y Competitividad (España)European CommissionConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]201820182017info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Postprintinfo:eu-repo/semantics/acceptedVersionhttp://hdl.handle.net/10261/160840reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52529-Rinfo:eu-repo/grantAgreement/EC/FP7/293770http://dx.doi.org/10.1016/j.molcel.2017.10.012Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1608402026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
title Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
spellingShingle Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
Frattini, Camilla
Cdc148/p97
DNA replication
Checkpoint
Chromosome dynamics
Cohesins
Genome integrity
Replication forks
Replication stress
title_short Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
title_full Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
title_fullStr Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
title_full_unstemmed Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
title_sort Cohesin ubiquitylation and mobilization facilitate stalled replication fork dynamics
dc.creator.none.fl_str_mv Frattini, Camilla
Villa, Sara
Pellicanò, Grazia
Jossen, Rachel
Katou, Yuki
Shirahige, Katsuhiko
Bermejo, Rodrigo
author Frattini, Camilla
author_facet Frattini, Camilla
Villa, Sara
Pellicanò, Grazia
Jossen, Rachel
Katou, Yuki
Shirahige, Katsuhiko
Bermejo, Rodrigo
author_role author
author2 Villa, Sara
Pellicanò, Grazia
Jossen, Rachel
Katou, Yuki
Shirahige, Katsuhiko
Bermejo, Rodrigo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Ciencia e Innovación (España)
Ministerio de Economía y Competitividad (España)
European Commission
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Cdc148/p97
DNA replication
Checkpoint
Chromosome dynamics
Cohesins
Genome integrity
Replication forks
Replication stress
topic Cdc148/p97
DNA replication
Checkpoint
Chromosome dynamics
Cohesins
Genome integrity
Replication forks
Replication stress
description 129 p.-7 fig.-7 fig.supl.-1 tab.supl.
publishDate 2017
dc.date.none.fl_str_mv 2017
2018
2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Postprint
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/160840
url http://hdl.handle.net/10261/160840
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/BFU2014-52529-R
info:eu-repo/grantAgreement/EC/FP7/293770
http://dx.doi.org/10.1016/j.molcel.2017.10.012
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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