Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet

High-energy (hyperlipidic) cafeteria diets induce insulin resistance limiting glucose oxidation, and lower amino acid catabolism. Despite high amino-N intake, amino acids are preserved, lowering urea excretion. We analysed how energy partition induced by cafeteria diet affects liver ammonium handlin...

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Detalhes bibliográficos
Autores: Agnelli, Silvia, Arriarán, Sofía, Oliva Lorenzo, Laia, Remesar Betlloch, Xavier, Fernández López, José Antonio, Alemany, Marià, 1946-
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2016
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/130027
Acesso em linha:https://hdl.handle.net/2445/130027
Access Level:Acceso aberto
Palavra-chave:Urea
Alimentació
Rates (Animals de laboratori)
Diet
Rats as laboratory animals
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spelling Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria dietAgnelli, SilviaArriarán, SofíaOliva Lorenzo, LaiaRemesar Betlloch, XavierFernández López, José AntonioAlemany, Marià, 1946-UreaAlimentacióRates (Animals de laboratori)UreaDietRats as laboratory animalsHigh-energy (hyperlipidic) cafeteria diets induce insulin resistance limiting glucose oxidation, and lower amino acid catabolism. Despite high amino-N intake, amino acids are preserved, lowering urea excretion. We analysed how energy partition induced by cafeteria diet affects liver ammonium handling and urea cycle. Female and male rats were fed control or cafeteria diets for 30 days. There was a remarkable constancy on enzyme activities and expressions of urea cycle and ammonium metabolism. The key enzymes controlling urea cycle: carbamoyl-P synthase 1, arginino-succinate synthase and arginase expressions were decreased by diet (albeit more markedly in males), and their activities were correlated with the gene expressions. The effects observed, in ammonium handling enzyme activities and expressions behaved in a way similar to that of the urea cycle, showing a generalized downregulation of liver amino acid catabolism. This process was affected by sex. The different strategies of amino-N handling by females and males further modulated the preservation of 2-amino N under sufficient available energy. The effects of sex were more marked than those of diet were, since different metabolism survival strategies changed substrate partition and fate. The data presented suggest a lower than expected N flow to the liver, which overall importance for amino acid metabolism tends to decrease with both cafeteria diet and female sex. Under standard conditions, liver availability of ammonium was low and controlled. The situation was unchanged (or even lowered) in cafeteria-fed rats, ultimately depending on intestinal amino acid catabolism.Royal Society of Chemistry2016info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/130027Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1039/c5ra25174eRSC Advances, 2016, vol. 6, num. 14, p. 11278-11288https://doi.org/10.1039/c5ra25174e(c) Agnelli, S. et al., 2016info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1300272026-05-27T06:46:51Z
dc.title.none.fl_str_mv Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
title Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
spellingShingle Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
Agnelli, Silvia
Urea
Alimentació
Rates (Animals de laboratori)
Urea
Diet
Rats as laboratory animals
title_short Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
title_full Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
title_fullStr Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
title_full_unstemmed Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
title_sort Modulation of rat liver urea cycle and related ammonium metabolism by sex and cafeteria diet
dc.creator.none.fl_str_mv Agnelli, Silvia
Arriarán, Sofía
Oliva Lorenzo, Laia
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
author Agnelli, Silvia
author_facet Agnelli, Silvia
Arriarán, Sofía
Oliva Lorenzo, Laia
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
author_role author
author2 Arriarán, Sofía
Oliva Lorenzo, Laia
Remesar Betlloch, Xavier
Fernández López, José Antonio
Alemany, Marià, 1946-
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Urea
Alimentació
Rates (Animals de laboratori)
Urea
Diet
Rats as laboratory animals
topic Urea
Alimentació
Rates (Animals de laboratori)
Urea
Diet
Rats as laboratory animals
description High-energy (hyperlipidic) cafeteria diets induce insulin resistance limiting glucose oxidation, and lower amino acid catabolism. Despite high amino-N intake, amino acids are preserved, lowering urea excretion. We analysed how energy partition induced by cafeteria diet affects liver ammonium handling and urea cycle. Female and male rats were fed control or cafeteria diets for 30 days. There was a remarkable constancy on enzyme activities and expressions of urea cycle and ammonium metabolism. The key enzymes controlling urea cycle: carbamoyl-P synthase 1, arginino-succinate synthase and arginase expressions were decreased by diet (albeit more markedly in males), and their activities were correlated with the gene expressions. The effects observed, in ammonium handling enzyme activities and expressions behaved in a way similar to that of the urea cycle, showing a generalized downregulation of liver amino acid catabolism. This process was affected by sex. The different strategies of amino-N handling by females and males further modulated the preservation of 2-amino N under sufficient available energy. The effects of sex were more marked than those of diet were, since different metabolism survival strategies changed substrate partition and fate. The data presented suggest a lower than expected N flow to the liver, which overall importance for amino acid metabolism tends to decrease with both cafeteria diet and female sex. Under standard conditions, liver availability of ammonium was low and controlled. The situation was unchanged (or even lowered) in cafeteria-fed rats, ultimately depending on intestinal amino acid catabolism.
publishDate 2016
dc.date.none.fl_str_mv 2016
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/130027
url https://hdl.handle.net/2445/130027
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1039/c5ra25174e
RSC Advances, 2016, vol. 6, num. 14, p. 11278-11288
https://doi.org/10.1039/c5ra25174e
dc.rights.none.fl_str_mv (c) Agnelli, S. et al., 2016
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Agnelli, S. et al., 2016
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Royal Society of Chemistry
publisher.none.fl_str_mv Royal Society of Chemistry
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,301629

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