Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation

Biodiversity loss is one major outcome of human-mediated ecosystem disturbance. One way that humans have triggered wildlife declines is by transporting disease-causing agents to remote areas of the world. Amphibians have been hit particularly hard by disease due in part to a globally distributed pat...

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Autores: Byrne, Allison Q., Vredenburg, Vance T., Martel, An, Pasmans, Frank, Bell, Rayna C., Blackburn, David C., Bletz, Molly C., Bosch, Jaime, Briggs, Cheryl J., Brown, Rafe M., Catenazzi, Alessandro, Familiar López, Mariel, Figueroa-Valenzuela, Raúl, Ghose, Sonia L., Jaeger, Jef R., Jani, Andrea J., Jirku, Miloslav, Knapp, Roland A., Muñoz, Antonio, Portik, Daniel M., Richards-Zawacki, Corine L., Rockney, Heidi, Rovito, Sean M., Sulaeman, Hasan, Tao, Nguyen Thien, Voyles, Jamie, Waddle, Anthony W., Yuan, Zhiyong, Rosenblum, Erica Bree
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/224491
Acesso em linha:http://hdl.handle.net/10261/224491
Access Level:acceso abierto
Palavra-chave:Batrachochytrium
dendrobatidis
Amphibians
Conservation
Genetic monitoring
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repository_id_str
spelling Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservationByrne, Allison Q.Vredenburg, Vance T.Martel, AnPasmans, FrankBell, Rayna C.Blackburn, David C.Bletz, Molly C.Bosch, JaimeBriggs, Cheryl J.Brown, Rafe M.Catenazzi, AlessandroFamiliar López, MarielFigueroa-Valenzuela, RaúlGhose, Sonia L.Jaeger, Jef R.Jani, Andrea J.Jirku, MiloslavKnapp, Roland A.Muñoz, AntonioPortik, Daniel M.Richards-Zawacki, Corine L.Rockney, HeidiRovito, Sean M.Sulaeman, HasanTao, Nguyen ThienVoyles, JamieWaddle, Anthony W.Yuan, ZhiyongRosenblum, Erica BreeBatrachochytriumdendrobatidisAmphibiansConservationGenetic monitoringBiodiversity loss is one major outcome of human-mediated ecosystem disturbance. One way that humans have triggered wildlife declines is by transporting disease-causing agents to remote areas of the world. Amphibians have been hit particularly hard by disease due in part to a globally distributed pathogenic chytrid fungus (Batrachochytrium dendrobatidis [Bd]). Prior research has revealed important insights into the biology and distribution of Bd; however, there are still many outstanding questions in this system. Although we know that there are multiple divergent lineages of Bd that differ in pathogenicity, we know little about how these lineages are distributed around the world and where lineages may be coming into contact. Here, we implement a custom genotyping method for a global set of Bd samples. This method is optimized to amplify and sequence degraded DNA from noninvasive skin swab samples. We describe a divergent lineage of Bd, which we call BdASIA3, that appears to be widespread in Southeast Asia. This lineage co-occurs with the global panzootic lineage (BdGPL) in multiple localities. Additionally, we shed light on the global distribution of BdGPL and highlight the expanded range of another lineage, BdCAPE. Finally,we argue that more monitoring needs to take place where Bd lineages are coming into contact and where we know little about Bd lineage diversity. Monitoring need not use expensive or difficult field techniques but can use archived swab samples to further explore the history—and predict the future impacts—of this devastating pathogen.This work was supported by NSF Grant IOS 1354241 (to E.B.R.), NSF Grant DEB 1557190 (to C.J.B., R.A.K., and E.B.R.), NSF Grant DEB 1551488 (to J.V., C.L.R.-Z., and E.B.R.), and the NSF Graduate Research Fellowship Program (A.Q.B.). Sequencing done at the University of Idaho Institute of Bioinformatics and Evolutionary Studies Genomics Resources Core is supported by NIH Centers of Biomedical Research Excellence Phase III Grant P30GM103324. Sample collection was supported by grants from the NSF (DEB-1202609 [to D.C.B.]; DEB-0743491, 0334952, 1418895, and 1654388 [to R.M.B.]; and Belmont Forum 1633948 [to V.T.V.]), the Czech Science Foundation and Institute of Parasitology (Grants P506/10/2330 and RVO 60077344 [to M.J.]), the National Natural Science Foundation of China (Grant 31702008 [to Z.Y.]), and the Yunnan Applied Basic Research Project (Grant 2018FD047 [to Z.Y.]).National Academy of Sciences (U.S.)National Science Foundation (US)Czech Science FoundationNational Natural Science Foundation of ChinaConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]2020202020192020info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionhttp://hdl.handle.net/10261/224491reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttp://dx.doi.org/10.1073/pnas.1908289116Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2244912026-05-22T06:33:51Z
dc.title.none.fl_str_mv Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
title Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
spellingShingle Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
Byrne, Allison Q.
Batrachochytrium
dendrobatidis
Amphibians
Conservation
Genetic monitoring
title_short Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
title_full Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
title_fullStr Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
title_full_unstemmed Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
title_sort Cryptic diversity of a widespread global pathogen reveals expanded threats to amphibian conservation
dc.creator.none.fl_str_mv Byrne, Allison Q.
Vredenburg, Vance T.
Martel, An
Pasmans, Frank
Bell, Rayna C.
Blackburn, David C.
Bletz, Molly C.
Bosch, Jaime
Briggs, Cheryl J.
Brown, Rafe M.
Catenazzi, Alessandro
Familiar López, Mariel
Figueroa-Valenzuela, Raúl
Ghose, Sonia L.
Jaeger, Jef R.
Jani, Andrea J.
Jirku, Miloslav
Knapp, Roland A.
Muñoz, Antonio
Portik, Daniel M.
Richards-Zawacki, Corine L.
Rockney, Heidi
Rovito, Sean M.
Sulaeman, Hasan
Tao, Nguyen Thien
Voyles, Jamie
Waddle, Anthony W.
Yuan, Zhiyong
Rosenblum, Erica Bree
author Byrne, Allison Q.
author_facet Byrne, Allison Q.
Vredenburg, Vance T.
Martel, An
Pasmans, Frank
Bell, Rayna C.
Blackburn, David C.
Bletz, Molly C.
Bosch, Jaime
Briggs, Cheryl J.
Brown, Rafe M.
Catenazzi, Alessandro
Familiar López, Mariel
Figueroa-Valenzuela, Raúl
Ghose, Sonia L.
Jaeger, Jef R.
Jani, Andrea J.
Jirku, Miloslav
Knapp, Roland A.
Muñoz, Antonio
Portik, Daniel M.
Richards-Zawacki, Corine L.
Rockney, Heidi
Rovito, Sean M.
Sulaeman, Hasan
Tao, Nguyen Thien
Voyles, Jamie
Waddle, Anthony W.
Yuan, Zhiyong
Rosenblum, Erica Bree
author_role author
author2 Vredenburg, Vance T.
Martel, An
Pasmans, Frank
Bell, Rayna C.
Blackburn, David C.
Bletz, Molly C.
Bosch, Jaime
Briggs, Cheryl J.
Brown, Rafe M.
Catenazzi, Alessandro
Familiar López, Mariel
Figueroa-Valenzuela, Raúl
Ghose, Sonia L.
Jaeger, Jef R.
Jani, Andrea J.
Jirku, Miloslav
Knapp, Roland A.
Muñoz, Antonio
Portik, Daniel M.
Richards-Zawacki, Corine L.
Rockney, Heidi
Rovito, Sean M.
Sulaeman, Hasan
Tao, Nguyen Thien
Voyles, Jamie
Waddle, Anthony W.
Yuan, Zhiyong
Rosenblum, Erica Bree
author2_role author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv National Science Foundation (US)
Czech Science Foundation
National Natural Science Foundation of China
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Batrachochytrium
dendrobatidis
Amphibians
Conservation
Genetic monitoring
topic Batrachochytrium
dendrobatidis
Amphibians
Conservation
Genetic monitoring
description Biodiversity loss is one major outcome of human-mediated ecosystem disturbance. One way that humans have triggered wildlife declines is by transporting disease-causing agents to remote areas of the world. Amphibians have been hit particularly hard by disease due in part to a globally distributed pathogenic chytrid fungus (Batrachochytrium dendrobatidis [Bd]). Prior research has revealed important insights into the biology and distribution of Bd; however, there are still many outstanding questions in this system. Although we know that there are multiple divergent lineages of Bd that differ in pathogenicity, we know little about how these lineages are distributed around the world and where lineages may be coming into contact. Here, we implement a custom genotyping method for a global set of Bd samples. This method is optimized to amplify and sequence degraded DNA from noninvasive skin swab samples. We describe a divergent lineage of Bd, which we call BdASIA3, that appears to be widespread in Southeast Asia. This lineage co-occurs with the global panzootic lineage (BdGPL) in multiple localities. Additionally, we shed light on the global distribution of BdGPL and highlight the expanded range of another lineage, BdCAPE. Finally,we argue that more monitoring needs to take place where Bd lineages are coming into contact and where we know little about Bd lineage diversity. Monitoring need not use expensive or difficult field techniques but can use archived swab samples to further explore the history—and predict the future impacts—of this devastating pathogen.
publishDate 2019
dc.date.none.fl_str_mv 2019
2020
2020
2020
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/224491
url http://hdl.handle.net/10261/224491
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv http://dx.doi.org/10.1073/pnas.1908289116

dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv National Academy of Sciences (U.S.)
publisher.none.fl_str_mv National Academy of Sciences (U.S.)
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
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