Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"

Supplementary files of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux ". This research paper describes the fluxome of 3-hydroxy...

Descripción completa

Detalles Bibliográficos
Autores: Fina Romero, Albert, Millard, Pierre, Albiol, Joan, Ferrer, Pau, Heux, Stephanie
Tipo de recurso: conjunto de datos
Fecha de publicación:2023
País:España
Institución:Consorci de Serveis Universitaris de Catalunya (CSUC)
Repositorio:CORA.Repositori de Dades de Recerca
OAI Identifier:oai:dnet:cora.rdr____::96fb24369945f053825246dfc543ffbe
Acceso en línea:https://doi.org/10.34810/DATA673
Access Level:acceso abierto
Palabra clave:Chemistry
Engineering
Fluxomics
13C-Metabolic Flux Analysis
13C-MFA
Pichia pastoris
High Throughput
3-hydroxypropionic acid
pH
id ES_4fecf4f6431d03b6cd1ee8d8506cf606
oai_identifier_str oai:dnet:cora.rdr____::96fb24369945f053825246dfc543ffbe
network_acronym_str ES
network_name_str España
repository_id_str
spelling Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"Fina Romero, AlbertMillard, PierreAlbiol, JoanFerrer, PauHeux, StephanieChemistryEngineeringFluxomics13C-Metabolic Flux Analysis13C-MFAPichia pastorisHigh Throughput3-hydroxypropionic acidpHSupplementary files of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux ". This research paper describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis).CORA.Repositori de Dades de RecercaUniversitat Autònoma de Barcelona. Biblioteca de Ciència i Tecnologia2023info:eu-repo/semantics/datasethttps://doi.org/10.34810/DATA673reponame:CORA.Repositori de Dades de Recercainstname:Consorci de Serveis Universitaris de Catalunya (CSUC)Inglésinfo:eu-repo/semantics/openAccessCC0 1.0oai:dnet:cora.rdr____::96fb24369945f053825246dfc543ffbe2026-06-17T12:20:17Z
dc.title.none.fl_str_mv Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
title Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
spellingShingle Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
Fina Romero, Albert
Chemistry
Engineering
Fluxomics
13C-Metabolic Flux Analysis
13C-MFA
Pichia pastoris
High Throughput
3-hydroxypropionic acid
pH
title_short Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
title_full Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
title_fullStr Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
title_full_unstemmed Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
title_sort Supplementary materials of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux"
dc.creator.none.fl_str_mv Fina Romero, Albert
Millard, Pierre
Albiol, Joan
Ferrer, Pau
Heux, Stephanie
author Fina Romero, Albert
author_facet Fina Romero, Albert
Millard, Pierre
Albiol, Joan
Ferrer, Pau
Heux, Stephanie
author_role author
author2 Millard, Pierre
Albiol, Joan
Ferrer, Pau
Heux, Stephanie
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Universitat Autònoma de Barcelona. Biblioteca de Ciència i Tecnologia
dc.subject.none.fl_str_mv Chemistry
Engineering
Fluxomics
13C-Metabolic Flux Analysis
13C-MFA
Pichia pastoris
High Throughput
3-hydroxypropionic acid
pH
topic Chemistry
Engineering
Fluxomics
13C-Metabolic Flux Analysis
13C-MFA
Pichia pastoris
High Throughput
3-hydroxypropionic acid
pH
description Supplementary files of research article "High throughput 13C-metabolic flux analysis of 3-hydroxypropionic acid producing Pichia pastoris reveals limited availability of acetyl-CoA and ATP due to tight control of the glycolytic flux ". This research paper describes the fluxome of 3-hydroxypropionic acid producing Pichia pastoris recombinant strains. Glycerol was used as a sole carbon source. A High Throughput platform was used to simultaneously characterize the fluxome of 10 recombinant strains at pH 5 and 6 recombinant strains at pH 3.5. All the experiments were carried in triplicates. To determine the intracellular fluxes, stationary 13C-Metabolic Flux Analysis was used using the data of the isotopologue distribution of the proteinogenic amino acids. The files attached herein contain: the description of how to obtain the models used for this study, the raw data obtained from the mini-bioreactor cultivations, the files to process the raw data, and the results (bioprocess parameters and fluxes from 13C-Metabolic flux analysis and Flux Balance Analysis).
publishDate 2023
dc.date.none.fl_str_mv 2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
format dataset
dc.identifier.none.fl_str_mv https://doi.org/10.34810/DATA673
url https://doi.org/10.34810/DATA673
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
CC0 1.0
eu_rights_str_mv openAccess
rights_invalid_str_mv CC0 1.0
dc.publisher.none.fl_str_mv CORA.Repositori de Dades de Recerca
publisher.none.fl_str_mv CORA.Repositori de Dades de Recerca
dc.source.none.fl_str_mv reponame:CORA.Repositori de Dades de Recerca
instname:Consorci de Serveis Universitaris de Catalunya (CSUC)
instname_str Consorci de Serveis Universitaris de Catalunya (CSUC)
reponame_str CORA.Repositori de Dades de Recerca
collection CORA.Repositori de Dades de Recerca
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407849543630848
score 15,812455

Similares en LA Referencia