Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures

Brominated organic compounds such as 1,2-dibromoethane (1,2-DBA) are highly toxic groundwater contaminants. Multi-element compound-specific isotope analysis bears the potential to elucidate the biodegradation pathways of 1,2-DBA in the environment, which is crucial information to assess its fate in...

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Autores: Palau, Jordi, Trueba-Santiso, Alba, Yu, R., Mortan, S, Shouakar-Stash, O., Freedman, D.L., Wasmund, K., Hunkeler, D., Marco-Urrea, E., Rosell Linares, Mònica
Tipo de recurso: artículo
Estado:Versión aceptada para publicación
Fecha de publicación:2023
País:España
Institución:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
Repositorio:Recercat. Dipósit de la Recerca de Catalunya
OAI Identifier:oai:recercat.cat:2445/195361
Acceso en línea:https://hdl.handle.net/2445/195361
Access Level:acceso abierto
Palabra clave:Biodegradació
Isòtops de carboni
Isòtops
Biodegradation
Carbon isotopes
Isotopes
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spelling Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing culturesPalau, JordiTrueba-Santiso, AlbaYu, R.Mortan, SShouakar-Stash, O.Freedman, D.L.Wasmund, K.Hunkeler, D.Marco-Urrea, E.Rosell Linares, MònicaBiodegradacióIsòtops de carboniIsòtopsBiodegradationCarbon isotopesIsotopesBrominated organic compounds such as 1,2-dibromoethane (1,2-DBA) are highly toxic groundwater contaminants. Multi-element compound-specific isotope analysis bears the potential to elucidate the biodegradation pathways of 1,2-DBA in the environment, which is crucial information to assess its fate in contaminated sites. This study investigates for the first time dual C−Br isotope fractionation during in vivo biodegradation of 1,2-DBA by two anaerobic enrichment cultures containing organohaliderespiring bacteria (i.e., either Dehalococcoides or Dehalogenimonas). Different εbulk C values (−1.8 ± 0.2 and −19.2 ± 3.5¿, respectively) were obtained, whereas their respective εbulk Br values were lower and similar to each other (−1.22 ± 0.08 and −1.2 ± 0.5¿), leading to distinctly different trends (ΛC−Br = Δδ13C/Δδ81Br ≈ εbulkC /εbulkBr ) in a dual C−Br isotope plot (1.4 ± 0.2 and 12 ± 4, respectively). These results suggest the occurrence of different underlying reaction mechanisms during enzymatic 1,2-DBA transformation, that is, concerted dihaloelimination and nucleophilic substitution (SN2-reaction). The strongly pathway-dependent ΛC−Br values illustrate the potential of this approach to elucidate the reaction mechanism of 1,2-DBA in the field and to select appropriate εbulkC values for quantification of biodegradation. The results of this study provide valuable information for future biodegradation studies of 1,2-DBA in contaminated sites.American Chemical Society2023202320232023info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersion10 p.application/pdfhttps://hdl.handle.net/2445/195361Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)reponame:Recercat. Dipósit de la Recerca de Catalunyainstname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)InglésReproducció del document publicat a: https://doi.org/10.1021/acs.est.2c07137Environmental Science & Technology, 2023, vol. 57, num. 5, p. 1949-1958https://doi.org/10.1021/acs.est.2c07137(c) The Authors , 2023http://creativecommons.org/licenses/by/3.0/es/info:eu-repo/semantics/openAccessoai:recercat.cat:2445/1953612026-05-29T05:05:01Z
dc.title.none.fl_str_mv Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
title Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
spellingShingle Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
Palau, Jordi
Biodegradació
Isòtops de carboni
Isòtops
Biodegradation
Carbon isotopes
Isotopes
title_short Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
title_full Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
title_fullStr Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
title_full_unstemmed Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
title_sort Dual C-Br isotope fractionation indicates distinct reductive dehalogenation mechanisms of 1,2-dibromoethane in Dehalococcoides- and Dehalogenimonas-containing cultures
dc.creator.none.fl_str_mv Palau, Jordi
Trueba-Santiso, Alba
Yu, R.
Mortan, S
Shouakar-Stash, O.
Freedman, D.L.
Wasmund, K.
Hunkeler, D.
Marco-Urrea, E.
Rosell Linares, Mònica
author Palau, Jordi
author_facet Palau, Jordi
Trueba-Santiso, Alba
Yu, R.
Mortan, S
Shouakar-Stash, O.
Freedman, D.L.
Wasmund, K.
Hunkeler, D.
Marco-Urrea, E.
Rosell Linares, Mònica
author_role author
author2 Trueba-Santiso, Alba
Yu, R.
Mortan, S
Shouakar-Stash, O.
Freedman, D.L.
Wasmund, K.
Hunkeler, D.
Marco-Urrea, E.
Rosell Linares, Mònica
author2_role author
author
author
author
author
author
author
author
author
dc.subject.none.fl_str_mv Biodegradació
Isòtops de carboni
Isòtops
Biodegradation
Carbon isotopes
Isotopes
topic Biodegradació
Isòtops de carboni
Isòtops
Biodegradation
Carbon isotopes
Isotopes
description Brominated organic compounds such as 1,2-dibromoethane (1,2-DBA) are highly toxic groundwater contaminants. Multi-element compound-specific isotope analysis bears the potential to elucidate the biodegradation pathways of 1,2-DBA in the environment, which is crucial information to assess its fate in contaminated sites. This study investigates for the first time dual C−Br isotope fractionation during in vivo biodegradation of 1,2-DBA by two anaerobic enrichment cultures containing organohaliderespiring bacteria (i.e., either Dehalococcoides or Dehalogenimonas). Different εbulk C values (−1.8 ± 0.2 and −19.2 ± 3.5¿, respectively) were obtained, whereas their respective εbulk Br values were lower and similar to each other (−1.22 ± 0.08 and −1.2 ± 0.5¿), leading to distinctly different trends (ΛC−Br = Δδ13C/Δδ81Br ≈ εbulkC /εbulkBr ) in a dual C−Br isotope plot (1.4 ± 0.2 and 12 ± 4, respectively). These results suggest the occurrence of different underlying reaction mechanisms during enzymatic 1,2-DBA transformation, that is, concerted dihaloelimination and nucleophilic substitution (SN2-reaction). The strongly pathway-dependent ΛC−Br values illustrate the potential of this approach to elucidate the reaction mechanism of 1,2-DBA in the field and to select appropriate εbulkC values for quantification of biodegradation. The results of this study provide valuable information for future biodegradation studies of 1,2-DBA in contaminated sites.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/195361
url https://hdl.handle.net/2445/195361
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: https://doi.org/10.1021/acs.est.2c07137
Environmental Science & Technology, 2023, vol. 57, num. 5, p. 1949-1958
https://doi.org/10.1021/acs.est.2c07137
dc.rights.none.fl_str_mv (c) The Authors , 2023
http://creativecommons.org/licenses/by/3.0/es/
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) The Authors , 2023
http://creativecommons.org/licenses/by/3.0/es/
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv 10 p.
application/pdf
dc.publisher.none.fl_str_mv American Chemical Society
publisher.none.fl_str_mv American Chemical Society
dc.source.none.fl_str_mv Articles publicats en revistes (Mineralogia, Petrologia i Geologia Aplicada)
reponame:Recercat. Dipósit de la Recerca de Catalunya
instname:Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
instname_str Varias* (Consorci de Biblioteques Universitáries de Catalunya, Centre de Serveis Científics i Acadèmics de Catalunya)
reponame_str Recercat. Dipósit de la Recerca de Catalunya
collection Recercat. Dipósit de la Recerca de Catalunya
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,198674

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