A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts

Introduction: Strigolactones (SLs) are the most representative germination stimulants for seeds of root parasitic plants, and they show activity even at concentrations below 10 M. The low amounts of stimulants produced by the host and their rapid degradability make it crucial to develop analytical m...

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Autores: Rial, Carlos, Varela, Rosa M., Molinillo, José M. G., López-Ráez, Juan A., Macías, Francisco A.
Formato: artículo
Fecha de publicación:2019
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/180641
Acesso em linha:http://hdl.handle.net/10261/180641
Access Level:acceso abierto
Palavra-chave:Parasitic plants
validation
Solanum lycopersicum
quantification
stimulators
strigolactones
tomato
UHPLC-MS/MS
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spelling A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extractsRial, CarlosVarela, Rosa M.Molinillo, José M. G.López-Ráez, Juan A.Macías, Francisco A.Parasitic plantsvalidationSolanum lycopersicumquantificationstimulatorsstrigolactonestomatoUHPLC-MS/MSIntroduction: Strigolactones (SLs) are the most representative germination stimulants for seeds of root parasitic plants, and they show activity even at concentrations below 10 M. The low amounts of stimulants produced by the host and their rapid degradability make it crucial to develop analytical methods with very low limits of quantification. Objective: To develop a sensitive and validated analytical method for the simultaneous quantification of seven SLs [7-oxoorobanchyl acetate (1), solanacol (2), orobanchol (4), strigol (5), fabacyl acetate (6), orobanchyl acetate (7), and 5-deoxystrigol (8)]. Methods: SLs were analysed using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), with (±)-GR24 (3) employed as internal standard (IS). Validation was based on selectivity, linearity, precision of the peak areas (repeatability and intermediate precision), detection and quantification limits, and stability. Results: A simple, rapid and reliable UHPLC-MS/MS method has been validated for the routine analysis of seven SLs and has been successfully applied to quantify them in exudates and extracts from tomato roots (Solanum lycopersicum). The limits of quantifications range from 0.05 μg/L for 5-deoxystrigol to 0.96 μg/L for solanacol. Conclusion: The method provides a useful tool for research in all the fields related to SLs, both for studies related to their function as hormones, and signalling molecules in the rhizosphere, without sample preparation required for extracts and root exudates in less than 11 minutes.The authors thank Professor Miguel Palma from the University of Cadiz for his comments and help with the manuscript. Ministerio de Economía, Industria y Competitividad (MINEICO) of Spain AGL2017‐88083‐R, AGL2015‐64990‐C2‐1‐R and AGL2013‐42238‐R.Peer ReviewedJohn Wiley & SonsMinisterio de Economía, Industria y Competitividad (España)2019201920192019info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501http://hdl.handle.net/10261/180641reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Inglés#PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE##PLACEHOLDER_PARENT_METADATA_VALUE#info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88083-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64990-C2-1-Rinfo:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-42238-Rhttps://onlinelibrary.wiley.com/doi/10.1002/pca.2796info:eu-repo/semantics/openAccessoai:digital.csic.es:10261/1806412026-05-22T06:33:51Z
dc.title.none.fl_str_mv A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
title A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
spellingShingle A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
Rial, Carlos
Parasitic plants
validation
Solanum lycopersicum
quantification
stimulators
strigolactones
tomato
UHPLC-MS/MS
title_short A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
title_full A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
title_fullStr A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
title_full_unstemmed A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
title_sort A new UHPLC-MS/MS method for the direct determination of strigolactones in root exudates and extracts
dc.creator.none.fl_str_mv Rial, Carlos
Varela, Rosa M.
Molinillo, José M. G.
López-Ráez, Juan A.
Macías, Francisco A.
author Rial, Carlos
author_facet Rial, Carlos
Varela, Rosa M.
Molinillo, José M. G.
López-Ráez, Juan A.
Macías, Francisco A.
author_role author
author2 Varela, Rosa M.
Molinillo, José M. G.
López-Ráez, Juan A.
Macías, Francisco A.
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Ministerio de Economía, Industria y Competitividad (España)
dc.subject.none.fl_str_mv Parasitic plants
validation
Solanum lycopersicum
quantification
stimulators
strigolactones
tomato
UHPLC-MS/MS
topic Parasitic plants
validation
Solanum lycopersicum
quantification
stimulators
strigolactones
tomato
UHPLC-MS/MS
description Introduction: Strigolactones (SLs) are the most representative germination stimulants for seeds of root parasitic plants, and they show activity even at concentrations below 10 M. The low amounts of stimulants produced by the host and their rapid degradability make it crucial to develop analytical methods with very low limits of quantification. Objective: To develop a sensitive and validated analytical method for the simultaneous quantification of seven SLs [7-oxoorobanchyl acetate (1), solanacol (2), orobanchol (4), strigol (5), fabacyl acetate (6), orobanchyl acetate (7), and 5-deoxystrigol (8)]. Methods: SLs were analysed using ultra-high performance liquid chromatography coupled to tandem mass spectrometry (UHPLC-MS/MS), with (±)-GR24 (3) employed as internal standard (IS). Validation was based on selectivity, linearity, precision of the peak areas (repeatability and intermediate precision), detection and quantification limits, and stability. Results: A simple, rapid and reliable UHPLC-MS/MS method has been validated for the routine analysis of seven SLs and has been successfully applied to quantify them in exudates and extracts from tomato roots (Solanum lycopersicum). The limits of quantifications range from 0.05 μg/L for 5-deoxystrigol to 0.96 μg/L for solanacol. Conclusion: The method provides a useful tool for research in all the fields related to SLs, both for studies related to their function as hormones, and signalling molecules in the rhizosphere, without sample preparation required for extracts and root exudates in less than 11 minutes.
publishDate 2019
dc.date.none.fl_str_mv 2019
2019
2019
2019
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
format article
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/180641
url http://hdl.handle.net/10261/180641
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv #PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
#PLACEHOLDER_PARENT_METADATA_VALUE#
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2017-88083-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2015-64990-C2-1-R
info:eu-repo/grantAgreement/MINECO/Plan Estatal de Investigación Científica y Técnica y de Innovación 2013-2016/AGL2013-42238-R
https://onlinelibrary.wiley.com/doi/10.1002/pca.2796
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv John Wiley & Sons
publisher.none.fl_str_mv John Wiley & Sons
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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