Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters

In this work, the use of DNA-stabilized fluorescent silver nanoclusters for the detection of target pyrimidine-rich DNA sequences by formation of parallel and antiparallel triplex structures is studied by molecular fluorescence spectroscopy. In the case of parallel triplexes, the probe DNA fragments...

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Detalhes bibliográficos
Autores: García, Juan Fernando, Reguera, David, Valls, Andrea, Aviñó, Anna, Domínguez, Arnau, Eritja Casadellà, Ramón, Gargallo, Raimundo
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2023
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/307827
Acesso em linha:http://hdl.handle.net/10261/307827
https://api.elsevier.com/content/abstract/scopus_id/85152908677
Access Level:acceso abierto
Palavra-chave:Silver nanoclusters
Antiparallel triplex DNA
Circular dichroism
Detection
Fluorescence
Nanoclusters
Parallel triplex DNA
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
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spelling Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclustersGarcía, Juan FernandoReguera, DavidValls, AndreaAviñó, AnnaDomínguez, ArnauEritja Casadellà, RamónGargallo, RaimundoSilver nanoclustersAntiparallel triplex DNACircular dichroismDetectionFluorescenceNanoclustersParallel triplex DNAhttp://metadata.un.org/sdg/3Ensure healthy lives and promote well-being for all at all agesIn this work, the use of DNA-stabilized fluorescent silver nanoclusters for the detection of target pyrimidine-rich DNA sequences by formation of parallel and antiparallel triplex structures is studied by molecular fluorescence spectroscopy. In the case of parallel triplexes, the probe DNA fragments are Watson-Crick stabilized hairpins, and whereas in the case of antiparallel triplexes, the probe fragments are reverse-Hoogsteen clamps. In all cases, the formation of the triplex structures has been assessed by means of polyacrylamide gel electrophoresis, circular dichroism, and molecular fluorescence spectroscopies, as well as multivariate data analysis methods. The results have shown that it is possible the detection of pyrimidine-rich sequences with an acceptable selectivity by using the approach based on the formation of antiparallel triplex structures.We thank funding from the Spanish Ministerio de Ciencia e Innovación (PID2019-107158GB-I00 and PID2020-118145RB-I00) and La Marató de TV3 Foundation (202110-30). Javier Pacheco (University of Barcelona) is also thanked for carrying out some of the fluorescence experiments.Peer reviewedElsevierConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232023info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Publisher's versioninfo:eu-repo/semantics/publishedVersionapplication/pdfhttp://hdl.handle.net/10261/307827https://api.elsevier.com/content/abstract/scopus_id/85152908677reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.saa.2023.122752Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3078272026-05-22T06:33:51Z
dc.title.none.fl_str_mv Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
title Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
spellingShingle Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
García, Juan Fernando
Silver nanoclusters
Antiparallel triplex DNA
Circular dichroism
Detection
Fluorescence
Nanoclusters
Parallel triplex DNA
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
title_short Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
title_full Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
title_fullStr Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
title_full_unstemmed Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
title_sort Detection of pyrimidine-rich DNA sequences based on the formation of parallel and antiparallel triplex DNA and fluorescent silver nanoclusters
dc.creator.none.fl_str_mv García, Juan Fernando
Reguera, David
Valls, Andrea
Aviñó, Anna
Domínguez, Arnau
Eritja Casadellà, Ramón
Gargallo, Raimundo
author García, Juan Fernando
author_facet García, Juan Fernando
Reguera, David
Valls, Andrea
Aviñó, Anna
Domínguez, Arnau
Eritja Casadellà, Ramón
Gargallo, Raimundo
author_role author
author2 Reguera, David
Valls, Andrea
Aviñó, Anna
Domínguez, Arnau
Eritja Casadellà, Ramón
Gargallo, Raimundo
author2_role author
author
author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Silver nanoclusters
Antiparallel triplex DNA
Circular dichroism
Detection
Fluorescence
Nanoclusters
Parallel triplex DNA
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
topic Silver nanoclusters
Antiparallel triplex DNA
Circular dichroism
Detection
Fluorescence
Nanoclusters
Parallel triplex DNA
http://metadata.un.org/sdg/3
Ensure healthy lives and promote well-being for all at all ages
description In this work, the use of DNA-stabilized fluorescent silver nanoclusters for the detection of target pyrimidine-rich DNA sequences by formation of parallel and antiparallel triplex structures is studied by molecular fluorescence spectroscopy. In the case of parallel triplexes, the probe DNA fragments are Watson-Crick stabilized hairpins, and whereas in the case of antiparallel triplexes, the probe fragments are reverse-Hoogsteen clamps. In all cases, the formation of the triplex structures has been assessed by means of polyacrylamide gel electrophoresis, circular dichroism, and molecular fluorescence spectroscopies, as well as multivariate data analysis methods. The results have shown that it is possible the detection of pyrimidine-rich sequences with an acceptable selectivity by using the approach based on the formation of antiparallel triplex structures.
publishDate 2023
dc.date.none.fl_str_mv 2023
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Publisher's version
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/307827
https://api.elsevier.com/content/abstract/scopus_id/85152908677
url http://hdl.handle.net/10261/307827
https://api.elsevier.com/content/abstract/scopus_id/85152908677
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.saa.2023.122752
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.812455

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