Evidence of an efflux pump in Serratia marcescens

Spontaneous mutants resistant to fluoroquinolones were obtained by exposing Serratia marcescens NIMA (wild-type strain) to increasing concentrations of ciprofloxacin both in liquid and on solid media. Frequencies of mutation ranged from 10-7 to 10-9. Active expulsion of antibiotic was explored as a...

ver descrição completa

Detalhes bibliográficos
Autores: Berlanga Herranz, Mercedes, Vázquez, J. L., Borrell Hernández, Jordi, Montero Barrientos, Ma. Teresa, Viñas, Miquel
Formato: artículo
Estado:Versión publicada
Fecha de publicación:2000
País:España
Recursos:Universidad de Barcelona
Repositorio:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/54944
Acesso em linha:https://hdl.handle.net/2445/54944
Access Level:acceso abierto
Palavra-chave:Resistència als medicaments
Antibiòtics
Serratia marcescens
Medicaments antibacterians
Drug resistance
Antibiotics
id ES_48130818d6285fccd306c805f9ca4d47
oai_identifier_str oai:diposit.ub.edu:2445/54944
network_acronym_str ES
network_name_str España
repository_id_str
spelling Evidence of an efflux pump in Serratia marcescensBerlanga Herranz, MercedesVázquez, J. L.Borrell Hernández, JordiMontero Barrientos, Ma. TeresaViñas, MiquelResistència als medicamentsAntibiòticsSerratia marcescensMedicaments antibacteriansDrug resistanceAntibioticsSerratia marcescensSpontaneous mutants resistant to fluoroquinolones were obtained by exposing Serratia marcescens NIMA (wild-type strain) to increasing concentrations of ciprofloxacin both in liquid and on solid media. Frequencies of mutation ranged from 10-7 to 10-9. Active expulsion of antibiotic was explored as a possible mechanism of resistance in mutants as well as changes in topoisomerase target genes. The role of extrusion mechanisms in determining the emergence of multidrug-resistant bacteria was also examined. Mutants resistant to high concentrations of fluoroquinolones had a single mutation in their gyrA QRDR sequences, whereas the moderate resistance in the rest of mutants was due to extrusion of the drugMary Ann Liebert, Inc.2000info:eu-repo/semantics/articleinfo:eu-repo/semantics/publishedVersionapplication/pdfhttps://hdl.handle.net/2445/54944Articles publicats en revistes (Patologia i Terapèutica Experimental)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésReproducció del document publicat a: http://dx.doi.org/10.1089/107662900419410Microbial Drug Resistance, 2000, vol. 6, num. 2, p. 111-117http://dx.doi.org/10.1089/107662900419410(c) Mary Ann Liebert, Inc., 2000info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/549442026-05-27T06:46:51Z
dc.title.none.fl_str_mv Evidence of an efflux pump in Serratia marcescens
title Evidence of an efflux pump in Serratia marcescens
spellingShingle Evidence of an efflux pump in Serratia marcescens
Berlanga Herranz, Mercedes
Resistència als medicaments
Antibiòtics
Serratia marcescens
Medicaments antibacterians
Drug resistance
Antibiotics
Serratia marcescens
title_short Evidence of an efflux pump in Serratia marcescens
title_full Evidence of an efflux pump in Serratia marcescens
title_fullStr Evidence of an efflux pump in Serratia marcescens
title_full_unstemmed Evidence of an efflux pump in Serratia marcescens
title_sort Evidence of an efflux pump in Serratia marcescens
dc.creator.none.fl_str_mv Berlanga Herranz, Mercedes
Vázquez, J. L.
Borrell Hernández, Jordi
Montero Barrientos, Ma. Teresa
Viñas, Miquel
author Berlanga Herranz, Mercedes
author_facet Berlanga Herranz, Mercedes
Vázquez, J. L.
Borrell Hernández, Jordi
Montero Barrientos, Ma. Teresa
Viñas, Miquel
author_role author
author2 Vázquez, J. L.
Borrell Hernández, Jordi
Montero Barrientos, Ma. Teresa
Viñas, Miquel
author2_role author
author
author
author
dc.subject.none.fl_str_mv Resistència als medicaments
Antibiòtics
Serratia marcescens
Medicaments antibacterians
Drug resistance
Antibiotics
Serratia marcescens
topic Resistència als medicaments
Antibiòtics
Serratia marcescens
Medicaments antibacterians
Drug resistance
Antibiotics
Serratia marcescens
description Spontaneous mutants resistant to fluoroquinolones were obtained by exposing Serratia marcescens NIMA (wild-type strain) to increasing concentrations of ciprofloxacin both in liquid and on solid media. Frequencies of mutation ranged from 10-7 to 10-9. Active expulsion of antibiotic was explored as a possible mechanism of resistance in mutants as well as changes in topoisomerase target genes. The role of extrusion mechanisms in determining the emergence of multidrug-resistant bacteria was also examined. Mutants resistant to high concentrations of fluoroquinolones had a single mutation in their gyrA QRDR sequences, whereas the moderate resistance in the rest of mutants was due to extrusion of the drug
publishDate 2000
dc.date.none.fl_str_mv 2000
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/publishedVersion
format article
status_str publishedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/54944
url https://hdl.handle.net/2445/54944
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Reproducció del document publicat a: http://dx.doi.org/10.1089/107662900419410
Microbial Drug Resistance, 2000, vol. 6, num. 2, p. 111-117
http://dx.doi.org/10.1089/107662900419410
dc.rights.none.fl_str_mv (c) Mary Ann Liebert, Inc., 2000
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Mary Ann Liebert, Inc., 2000
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Mary Ann Liebert, Inc.
publisher.none.fl_str_mv Mary Ann Liebert, Inc.
dc.source.none.fl_str_mv Articles publicats en revistes (Patologia i Terapèutica Experimental)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869407336508948480
score 15,198674

Registos relacionados