Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli

White adipose tissue can metabolize large amounts of glucose to glycerol and lactate. We quantitatively traced glucose label to lactate, glycerol and fats in primary cultures of mature rat epididymal adipocytes. Cells were incubated with 7/14 mM 14C-glucose for 24/48 h. Medium metabolites and the la...

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Detalhes bibliográficos
Autores: Ho-Palma, Ana Cecilia, Rotondo, Floriana, Romero Romero, María del Mar, Fernández López, José Antonio, Remesar Betlloch, Xavier, Alemany, Marià, 1946-
Tipo de documento: artigo
Estado:Versión aceptada para publicación
Data de publicação:2018
País:España
Recursos:Universidad de Barcelona
Repositório:Dipòsit Digital de la UB
OAI Identifier:oai:diposit.ub.edu:2445/123707
Acesso em linha:https://hdl.handle.net/2445/123707
Access Level:Acceso aberto
Palavra-chave:Teixit adipós
Metabolisme dels lípids
Glucosa
Rates (Animals de laboratori)
Adipose tissues
Lipid metabolism
Glucose
Rats as laboratory animals
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spelling Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuliHo-Palma, Ana CeciliaRotondo, FlorianaRomero Romero, María del MarFernández López, José AntonioRemesar Betlloch, XavierAlemany, Marià, 1946-Teixit adipósMetabolisme dels lípidsGlucosaRates (Animals de laboratori)Adipose tissuesLipid metabolismGlucoseRats as laboratory animalsWhite adipose tissue can metabolize large amounts of glucose to glycerol and lactate. We quantitatively traced glucose label to lactate, glycerol and fats in primary cultures of mature rat epididymal adipocytes. Cells were incubated with 7/14 mM 14C-glucose for 24/48 h. Medium metabolites and the label in them and in cells' components were measured. Gene expression analysis was done using parallel incubations. Glucose concentration did not affect lactate efflux and most parameters. Glycerol efflux increased after 24h, coinciding with arrested lipogenesis. Steady production of lactate was maintained in parallel to glycerogenesis. Changes in adipocyte metabolism were paralleled by gene expression. Glucose use for lipogenesis was minimal, and stopped (24h-onwards) when glycerol efflux increased because of triacylglycerol turnover. Lactate steady efflux showed that anaerobic glycolysis was the main adipocyte source of energy. We can assume that adipose tissue may play a quantitatively significant effect on glycaemia, returning 3C fragments thus minimizing lipogenesis.Taylor and Francis2018info:eu-repo/semantics/articleinfo:eu-repo/semantics/acceptedVersionapplication/pdfhttps://hdl.handle.net/2445/123707Articles publicats en revistes (Bioquímica i Biomedicina Molecular)reponame:Dipòsit Digital de la UBinstname:Universidad de BarcelonaInglésVersió postprint del document publicat a: https://doi.org/10.1080/21623945.2018.1460020Adipocyte, 2018https://doi.org/10.1080/21623945.2018.1460020(c) Taylor and Francis, 2018info:eu-repo/semantics/openAccessoai:diposit.ub.edu:2445/1237072026-05-27T06:46:51Z
dc.title.none.fl_str_mv Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
title Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
spellingShingle Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
Ho-Palma, Ana Cecilia
Teixit adipós
Metabolisme dels lípids
Glucosa
Rates (Animals de laboratori)
Adipose tissues
Lipid metabolism
Glucose
Rats as laboratory animals
title_short Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
title_full Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
title_fullStr Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
title_full_unstemmed Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
title_sort Use of 14C-glucose by primary cultures of mature rat epididymal adipocytes. Marked release of lactate and glycerol, but limited lipogenesis in the absence of external stimuli
dc.creator.none.fl_str_mv Ho-Palma, Ana Cecilia
Rotondo, Floriana
Romero Romero, María del Mar
Fernández López, José Antonio
Remesar Betlloch, Xavier
Alemany, Marià, 1946-
author Ho-Palma, Ana Cecilia
author_facet Ho-Palma, Ana Cecilia
Rotondo, Floriana
Romero Romero, María del Mar
Fernández López, José Antonio
Remesar Betlloch, Xavier
Alemany, Marià, 1946-
author_role author
author2 Rotondo, Floriana
Romero Romero, María del Mar
Fernández López, José Antonio
Remesar Betlloch, Xavier
Alemany, Marià, 1946-
author2_role author
author
author
author
author
dc.subject.none.fl_str_mv Teixit adipós
Metabolisme dels lípids
Glucosa
Rates (Animals de laboratori)
Adipose tissues
Lipid metabolism
Glucose
Rats as laboratory animals
topic Teixit adipós
Metabolisme dels lípids
Glucosa
Rates (Animals de laboratori)
Adipose tissues
Lipid metabolism
Glucose
Rats as laboratory animals
description White adipose tissue can metabolize large amounts of glucose to glycerol and lactate. We quantitatively traced glucose label to lactate, glycerol and fats in primary cultures of mature rat epididymal adipocytes. Cells were incubated with 7/14 mM 14C-glucose for 24/48 h. Medium metabolites and the label in them and in cells' components were measured. Gene expression analysis was done using parallel incubations. Glucose concentration did not affect lactate efflux and most parameters. Glycerol efflux increased after 24h, coinciding with arrested lipogenesis. Steady production of lactate was maintained in parallel to glycerogenesis. Changes in adipocyte metabolism were paralleled by gene expression. Glucose use for lipogenesis was minimal, and stopped (24h-onwards) when glycerol efflux increased because of triacylglycerol turnover. Lactate steady efflux showed that anaerobic glycolysis was the main adipocyte source of energy. We can assume that adipose tissue may play a quantitatively significant effect on glycaemia, returning 3C fragments thus minimizing lipogenesis.
publishDate 2018
dc.date.none.fl_str_mv 2018
dc.type.none.fl_str_mv info:eu-repo/semantics/article
info:eu-repo/semantics/acceptedVersion
format article
status_str acceptedVersion
dc.identifier.none.fl_str_mv https://hdl.handle.net/2445/123707
url https://hdl.handle.net/2445/123707
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Versió postprint del document publicat a: https://doi.org/10.1080/21623945.2018.1460020
Adipocyte, 2018
https://doi.org/10.1080/21623945.2018.1460020
dc.rights.none.fl_str_mv (c) Taylor and Francis, 2018
info:eu-repo/semantics/openAccess
rights_invalid_str_mv (c) Taylor and Francis, 2018
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv application/pdf
dc.publisher.none.fl_str_mv Taylor and Francis
publisher.none.fl_str_mv Taylor and Francis
dc.source.none.fl_str_mv Articles publicats en revistes (Bioquímica i Biomedicina Molecular)
reponame:Dipòsit Digital de la UB
instname:Universidad de Barcelona
instname_str Universidad de Barcelona
reponame_str Dipòsit Digital de la UB
collection Dipòsit Digital de la UB
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15.223283

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