Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF

Rusts are among the most important foliar biotrophic fungal diseases in legumes. Lathyrus cicera crop can be severely damaged by Uromyces pisi, to which partial resistance has been identified. Nevertheless, the underlying genetic basis and molecular mechanisms of this resistance are poorly understoo...

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Autores: Santos, Carmen, Martins, Davide Coelho, González-Bernal, María José, Rubiales, Diego, Vaz Patto, María Carlota
Tipo de documento: conjunto de datos
Data de publicação:2022
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositório:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/330626
Acesso em linha:http://hdl.handle.net/10261/330626
Access Level:Acceso aberto
Palavra-chave:Quantitative trait loci-QTL
Expression QTL-eQTL
Lathyrus cicera
Uromyces pisi
Partial resistance
QTL hotspots
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spelling Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIFSantos, CarmenMartins, Davide CoelhoGonzález-Bernal, María JoséRubiales, DiegoVaz Patto, María CarlotaQuantitative trait loci-QTLExpression QTL-eQTLLathyrus ciceraUromyces pisiPartial resistanceQTL hotspotsRusts are among the most important foliar biotrophic fungal diseases in legumes. Lathyrus cicera crop can be severely damaged by Uromyces pisi, to which partial resistance has been identified. Nevertheless, the underlying genetic basis and molecular mechanisms of this resistance are poorly understood in L. cicera. To prioritise the causative variants controlling partial resistance to rust in L. cicera, a recombinant inbred line (RIL) population, segregating for response to this pathogen, was used to combine the detection of related phenotypic- and expression-quantitative trait loci (pQTLs and eQTLs, respectively). RILs’ U. pisi disease severity (DS) was recorded in three independent screenings at seedling (growth chamber) and in one season of exploratory screening at adult plant stage (semi-controlled field conditions). A continuous DS range was observed in both conditions and used for pQTL mapping. Different pQTLs were identified under the growth chamber and semi-controlled field conditions, indicating a distinct genetic basis depending on the plant developmental stage and/or the environment. Additionally, the expression of nine genes related to U. pisi resistance in L. cicera was quantified for each RIL individual and used for eQTL mapping. One cis-eQTL and one trans-eQTL were identified controlling the expression variation of one gene related to rust resistance – a member of glycosyl hydrolase family 17. Integrating phenotyping, gene expression and linkage mapping allowed prioritising four candidate genes relevant for disease-resistance precision breeding involved in adaptation to biotic stress, cellular, and organelle homeostasis, and proteins directly involved in plant defence.Peer reviewedFigshareConsejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202320232022info:eu-repo/semantics/datasethttp://purl.org/coar/resource_type/c_ddb1image/tiffhttp://hdl.handle.net/10261/330626reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)InglésSantos, Carmen; Martins, Davide Coelho; González-Bernal, María José; Rubiales, Diego; Vaz Patto, María Carlota. Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea. https://doi.org/10.3389/fpls.2022.837613 . http://hdl.handle.net/10261/286788https://doi.org/10.3389/fpls.2022.837613.s003Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/3306262026-05-22T06:33:51Z
dc.title.none.fl_str_mv Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
title Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
spellingShingle Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
Santos, Carmen
Quantitative trait loci-QTL
Expression QTL-eQTL
Lathyrus cicera
Uromyces pisi
Partial resistance
QTL hotspots
title_short Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
title_full Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
title_fullStr Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
title_full_unstemmed Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
title_sort Image_2_Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea.TIF
dc.creator.none.fl_str_mv Santos, Carmen
Martins, Davide Coelho
González-Bernal, María José
Rubiales, Diego
Vaz Patto, María Carlota
author Santos, Carmen
author_facet Santos, Carmen
Martins, Davide Coelho
González-Bernal, María José
Rubiales, Diego
Vaz Patto, María Carlota
author_role author
author2 Martins, Davide Coelho
González-Bernal, María José
Rubiales, Diego
Vaz Patto, María Carlota
author2_role author
author
author
author
dc.contributor.none.fl_str_mv Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Quantitative trait loci-QTL
Expression QTL-eQTL
Lathyrus cicera
Uromyces pisi
Partial resistance
QTL hotspots
topic Quantitative trait loci-QTL
Expression QTL-eQTL
Lathyrus cicera
Uromyces pisi
Partial resistance
QTL hotspots
description Rusts are among the most important foliar biotrophic fungal diseases in legumes. Lathyrus cicera crop can be severely damaged by Uromyces pisi, to which partial resistance has been identified. Nevertheless, the underlying genetic basis and molecular mechanisms of this resistance are poorly understood in L. cicera. To prioritise the causative variants controlling partial resistance to rust in L. cicera, a recombinant inbred line (RIL) population, segregating for response to this pathogen, was used to combine the detection of related phenotypic- and expression-quantitative trait loci (pQTLs and eQTLs, respectively). RILs’ U. pisi disease severity (DS) was recorded in three independent screenings at seedling (growth chamber) and in one season of exploratory screening at adult plant stage (semi-controlled field conditions). A continuous DS range was observed in both conditions and used for pQTL mapping. Different pQTLs were identified under the growth chamber and semi-controlled field conditions, indicating a distinct genetic basis depending on the plant developmental stage and/or the environment. Additionally, the expression of nine genes related to U. pisi resistance in L. cicera was quantified for each RIL individual and used for eQTL mapping. One cis-eQTL and one trans-eQTL were identified controlling the expression variation of one gene related to rust resistance – a member of glycosyl hydrolase family 17. Integrating phenotyping, gene expression and linkage mapping allowed prioritising four candidate genes relevant for disease-resistance precision breeding involved in adaptation to biotic stress, cellular, and organelle homeostasis, and proteins directly involved in plant defence.
publishDate 2022
dc.date.none.fl_str_mv 2022
2023
2023
dc.type.none.fl_str_mv info:eu-repo/semantics/dataset
http://purl.org/coar/resource_type/c_ddb1
format dataset
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/330626
url http://hdl.handle.net/10261/330626
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv Santos, Carmen; Martins, Davide Coelho; González-Bernal, María José; Rubiales, Diego; Vaz Patto, María Carlota. Integrating Phenotypic and Gene Expression Linkage Mapping to Dissect Rust Resistance in Chickling Pea. https://doi.org/10.3389/fpls.2022.837613 . http://hdl.handle.net/10261/286788
https://doi.org/10.3389/fpls.2022.837613.s003
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.format.none.fl_str_mv image/tiff
dc.publisher.none.fl_str_mv Figshare
publisher.none.fl_str_mv Figshare
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
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score 15,228081

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