Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies
The brucellae are Gram-negative pathogens that cause brucellosis, a zoonosis of worldwide importance. The genus Brucella includes smooth and rough species that differ in that they carry smooth and rough lipopolysaccharides, respectively. Brucella abortus, B. melitensis, and B. suis are typical smoot...
| Autores: | , , , , , |
|---|---|
| Tipo de recurso: | artículo |
| Fecha de publicación: | 2012 |
| País: | España |
| Institución: | Universidad de Navarra |
| Repositorio: | Dadun. Depósito Académico Digital de la Universidad de Navarra |
| Idioma: | inglés |
| OAI Identifier: | oai:dadun.unav.edu:10171/29513 |
| Acceso en línea: | https://hdl.handle.net/10171/29513 |
| Access Level: | acceso abierto |
| Palabra clave: | Bacterial Proteins metabolism Brucella enzymology Gene Deletion Gene Expression Regulation Bacterial physiology Glycosyltransferases metabolism |
| id |
ES_432a8d3d916f7b7ed37f73d96f52c8ab |
|---|---|
| oai_identifier_str |
oai:dadun.unav.edu:10171/29513 |
| network_acronym_str |
ES |
| network_name_str |
España |
| repository_id_str |
|
| spelling |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella coloniesMancilla, M. (Marcos)|||/items/ce209889-0768-40f7-b2c5-00037ffda5a0Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efaZarraga, A.M. (Ana María)|||/items/912b620c-8ffa-4ff6-9c47-fb073e60b6e6López-Goñi, I. (Ignacio)|||/items/228678b8-8965-4c7c-aa6f-493277285d88Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7bBacterial Proteins metabolismBrucella enzymologyGene DeletionGene Expression RegulationBacterial physiologyGlycosyltransferases metabolismThe brucellae are Gram-negative pathogens that cause brucellosis, a zoonosis of worldwide importance. The genus Brucella includes smooth and rough species that differ in that they carry smooth and rough lipopolysaccharides, respectively. Brucella abortus, B. melitensis, and B. suis are typical smooth species. However, these smooth brucellae dissociate into rough mutants devoid of the lipopolysaccharide O-polysaccharide, a major antigen and a virulence determinant encoded in regions wbo (included in genomic island-2) and wbk. We demonstrate here the occurrence of spontaneous recombination events in those three Brucella species leading to the deletion of a 5.5-kb fragment carrying the wbkA glycosyltranferase gene and to the appearance of rough mutants. Analysis of the recombination intermediates suggested homologous recombination between the ISBm1 insertion sequences flanking wbkA as the mechanism generating the deletion. Excision of wbkA was reduced but not abrogated in a recA-deficient mutant, showing the existence of both RecA-dependent and -independent processes. Although the involvement of the ISBm1 copies flanking wbkA suggested a transpositional event, the predicted transpositional joint could not be detected. This absence of detectable transposition was consistent with the presence of polymorphism in the inverted repeats of one of the ISBm1 copies. The spontaneous excision of wbkA represents a novel dissociation mechanism of smooth brucellae that adds to the previously described excision of genomic island-2. This ISBm1-mediated wbkA excision and the different %GC levels of the excised fragment and of other wbk genes suggest that the Brucella wbk locus is the result of at least two horizontal acquisition events.American Society for MicrobiologyDadun. Depósito Académico Digital Universidad de Navarra20132013-07-1920122012-01-0120122012-01-01journal articlehttp://purl.org/coar/resource_type/c_6501info:eu-repo/semantics/articleapplication/pdfhttps://hdl.handle.net/10171/29513reponame:Dadun. Depósito Académico Digital de la Universidad de Navarrainstname:Universidad de NavarraInglésengopen accesshttp://purl.org/coar/access_right/c_abf2info:eu-repo/semantics/openAccessoai:dadun.unav.edu:10171/295132026-06-21T12:47:57Z |
| dc.title.none.fl_str_mv |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| title |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| spellingShingle |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies Mancilla, M. (Marcos)|||/items/ce209889-0768-40f7-b2c5-00037ffda5a0 Bacterial Proteins metabolism Brucella enzymology Gene Deletion Gene Expression Regulation Bacterial physiology Glycosyltransferases metabolism |
| title_short |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| title_full |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| title_fullStr |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| title_full_unstemmed |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| title_sort |
Spontaneous excision of the O-polysaccharide wbkA glycosyltranferase gene is a cause of dissociation of smooth to rough Brucella colonies |
| dc.creator.none.fl_str_mv |
Mancilla, M. (Marcos)|||/items/ce209889-0768-40f7-b2c5-00037ffda5a0 Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5 Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa Zarraga, A.M. (Ana María)|||/items/912b620c-8ffa-4ff6-9c47-fb073e60b6e6 López-Goñi, I. (Ignacio)|||/items/228678b8-8965-4c7c-aa6f-493277285d88 Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b |
| author |
Mancilla, M. (Marcos)|||/items/ce209889-0768-40f7-b2c5-00037ffda5a0 |
| author_facet |
Mancilla, M. (Marcos)|||/items/ce209889-0768-40f7-b2c5-00037ffda5a0 Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5 Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa Zarraga, A.M. (Ana María)|||/items/912b620c-8ffa-4ff6-9c47-fb073e60b6e6 López-Goñi, I. (Ignacio)|||/items/228678b8-8965-4c7c-aa6f-493277285d88 Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b |
| author_role |
author |
| author2 |
Marin, C.M. (C. M.)|||/items/8e4a7762-7bd7-487e-95bd-ab4777270aa5 Blasco, J.M. (J. M.)|||/items/63aa30f3-7ffa-4d2e-8dbd-d070fd4e4efa Zarraga, A.M. (Ana María)|||/items/912b620c-8ffa-4ff6-9c47-fb073e60b6e6 López-Goñi, I. (Ignacio)|||/items/228678b8-8965-4c7c-aa6f-493277285d88 Moriyon, I. (Ignacio)|||/items/834b4ce9-a879-4ba7-8884-39d0ddd88c7b |
| author2_role |
author author author author author |
| dc.contributor.none.fl_str_mv |
Dadun. Depósito Académico Digital Universidad de Navarra |
| dc.subject.none.fl_str_mv |
Bacterial Proteins metabolism Brucella enzymology Gene Deletion Gene Expression Regulation Bacterial physiology Glycosyltransferases metabolism |
| topic |
Bacterial Proteins metabolism Brucella enzymology Gene Deletion Gene Expression Regulation Bacterial physiology Glycosyltransferases metabolism |
| description |
The brucellae are Gram-negative pathogens that cause brucellosis, a zoonosis of worldwide importance. The genus Brucella includes smooth and rough species that differ in that they carry smooth and rough lipopolysaccharides, respectively. Brucella abortus, B. melitensis, and B. suis are typical smooth species. However, these smooth brucellae dissociate into rough mutants devoid of the lipopolysaccharide O-polysaccharide, a major antigen and a virulence determinant encoded in regions wbo (included in genomic island-2) and wbk. We demonstrate here the occurrence of spontaneous recombination events in those three Brucella species leading to the deletion of a 5.5-kb fragment carrying the wbkA glycosyltranferase gene and to the appearance of rough mutants. Analysis of the recombination intermediates suggested homologous recombination between the ISBm1 insertion sequences flanking wbkA as the mechanism generating the deletion. Excision of wbkA was reduced but not abrogated in a recA-deficient mutant, showing the existence of both RecA-dependent and -independent processes. Although the involvement of the ISBm1 copies flanking wbkA suggested a transpositional event, the predicted transpositional joint could not be detected. This absence of detectable transposition was consistent with the presence of polymorphism in the inverted repeats of one of the ISBm1 copies. The spontaneous excision of wbkA represents a novel dissociation mechanism of smooth brucellae that adds to the previously described excision of genomic island-2. This ISBm1-mediated wbkA excision and the different %GC levels of the excised fragment and of other wbk genes suggest that the Brucella wbk locus is the result of at least two horizontal acquisition events. |
| publishDate |
2012 |
| dc.date.none.fl_str_mv |
2012 2012-01-01 2012 2012-01-01 2013 2013-07-19 |
| dc.type.none.fl_str_mv |
journal article http://purl.org/coar/resource_type/c_6501 |
| dc.type.openaire.fl_str_mv |
info:eu-repo/semantics/article |
| format |
article |
| dc.identifier.none.fl_str_mv |
https://hdl.handle.net/10171/29513 |
| url |
https://hdl.handle.net/10171/29513 |
| dc.language.none.fl_str_mv |
Inglés eng |
| language_invalid_str_mv |
Inglés |
| language |
eng |
| dc.rights.none.fl_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| dc.rights.openaire.fl_str_mv |
info:eu-repo/semantics/openAccess |
| rights_invalid_str_mv |
open access http://purl.org/coar/access_right/c_abf2 |
| eu_rights_str_mv |
openAccess |
| dc.format.none.fl_str_mv |
application/pdf |
| dc.publisher.none.fl_str_mv |
American Society for Microbiology |
| publisher.none.fl_str_mv |
American Society for Microbiology |
| dc.source.none.fl_str_mv |
reponame:Dadun. Depósito Académico Digital de la Universidad de Navarra instname:Universidad de Navarra |
| instname_str |
Universidad de Navarra |
| reponame_str |
Dadun. Depósito Académico Digital de la Universidad de Navarra |
| collection |
Dadun. Depósito Académico Digital de la Universidad de Navarra |
| repository.name.fl_str_mv |
|
| repository.mail.fl_str_mv |
|
| _version_ |
1869407004943974400 |
| score |
15,301629 |