Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida

37 p.-6 fig.-2 tab.-1 graph. abst.

Detalhes bibliográficos
Autores: Rouches, Elsa, Gómez-Álvarez, Helena, Majira, A., Martín-Moldes, Zaira, Nogales, Juan, Díaz, Eduardo, Bugg, T.D.H., Baumberger, Stéphanie
Formato: artículo
Estado:Versión enviada para evaluación y publicación
Fecha de publicación:2021
País:España
Recursos:Consejo Superior de Investigaciones Científicas (CSIC)
Repositorio:DIGITAL.CSIC. Repositorio Institucional del CSIC
OAI Identifier:oai:digital.csic.es:10261/253023
Acesso em linha:http://hdl.handle.net/10261/253023
Access Level:acceso abierto
Palavra-chave:Bacterial conversion
Depolymerization
HPSEC
Lignin
Phenolic metabolites
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spelling Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putidaRouches, ElsaGómez-Álvarez, HelenaMajira, A.Martín-Moldes, ZairaNogales, JuanDíaz, EduardoBugg, T.D.H.Baumberger, StéphanieBacterial conversionDepolymerizationHPSECLigninPhenolic metabolites37 p.-6 fig.-2 tab.-1 graph. abst.In order to better understand bacterial depolymerization of lignin, a new analytical approach was proposed using Pseudomonas putida KT2440 as delignifying bacterium and Escherichia coli as non-delignifying control. Two different types of lignins, technical Kraft lignin and synthetic dehydrogenopolymer (DHP), were submitted to a bioconversion kinetic study over 7 days. The concomitant analysis of the supernatant acid-precipitable lignin fraction and water-soluble extractives by high-performance size-exclusion chromatography (HPSEC) and gas chromatography – mass spectrometry (GC–MS) highlighted the specific action of P. putida towards these substrates, with the transitory formation of phenolic metabolites (dihydroferulic acid for Kraft lignins and dimers for DHP) and the prevention of Kraft lignin self-assemblying. In both cases lignin apparent depolymerization followed by repolymerization was observed. The analysis of the bacterial pellets indicated the time-increasing content of lignins associated to bacterial cells, which could account for the apparent structural changes observed with E. coli in the supernatant.This work was supported by ADEME [N° 1401C0066] and by the European Commission [ERA-IB-14-055] in the framework of the LIGBIO project. The IJPB benefits from the support of the LabEx Saclay Plant Sciences-SPS (ANR-10-LABX-0040-SPS). Research in TDHB's group was supported by BBSRC grant BB/M025772/1.Peer reviewedElsevierAgence de la Transition Écologique (France)European CommissionLabEx SPSBiotechnology and Biological Sciences Research Council (UK)Rouches, Elsa [0000-0002-2411-4374]Gómez-Álvarez, Helena [0000-0002-2169-6778]Martín-Moldes, Zaira [0000-0002-2932-8064]Nogales, Juan [0000-0002-4961-0833]Díaz, Eduardo [0000-0002-9731-6524]Baumberger, Stéphanie [0000-0002-9550-4935]Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]202120212021info:eu-repo/semantics/articlehttp://purl.org/coar/resource_type/c_6501Preprintinfo:eu-repo/semantics/submittedVersionhttp://hdl.handle.net/10261/253023reponame:DIGITAL.CSIC. Repositorio Institucional del CSICinstname:Consejo Superior de Investigaciones Científicas (CSIC)Ingléshttps://doi.org/10.1016/j.biteb.2021.100742Síinfo:eu-repo/semantics/openAccessoai:digital.csic.es:10261/2530232026-05-22T06:33:51Z
dc.title.none.fl_str_mv Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
title Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
spellingShingle Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
Rouches, Elsa
Bacterial conversion
Depolymerization
HPSEC
Lignin
Phenolic metabolites
title_short Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
title_full Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
title_fullStr Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
title_full_unstemmed Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
title_sort Assessment strategy for bacterial lignin depolymerization: Kraft lignin and synthetic lignin bioconversion with Pseudomonas putida
dc.creator.none.fl_str_mv Rouches, Elsa
Gómez-Álvarez, Helena
Majira, A.
Martín-Moldes, Zaira
Nogales, Juan
Díaz, Eduardo
Bugg, T.D.H.
Baumberger, Stéphanie
author Rouches, Elsa
author_facet Rouches, Elsa
Gómez-Álvarez, Helena
Majira, A.
Martín-Moldes, Zaira
Nogales, Juan
Díaz, Eduardo
Bugg, T.D.H.
Baumberger, Stéphanie
author_role author
author2 Gómez-Álvarez, Helena
Majira, A.
Martín-Moldes, Zaira
Nogales, Juan
Díaz, Eduardo
Bugg, T.D.H.
Baumberger, Stéphanie
author2_role author
author
author
author
author
author
author
dc.contributor.none.fl_str_mv Agence de la Transition Écologique (France)
European Commission
LabEx SPS
Biotechnology and Biological Sciences Research Council (UK)
Rouches, Elsa [0000-0002-2411-4374]
Gómez-Álvarez, Helena [0000-0002-2169-6778]
Martín-Moldes, Zaira [0000-0002-2932-8064]
Nogales, Juan [0000-0002-4961-0833]
Díaz, Eduardo [0000-0002-9731-6524]
Baumberger, Stéphanie [0000-0002-9550-4935]
Consejo Superior de Investigaciones Científicas [https://ror.org/02gfc7t72]
dc.subject.none.fl_str_mv Bacterial conversion
Depolymerization
HPSEC
Lignin
Phenolic metabolites
topic Bacterial conversion
Depolymerization
HPSEC
Lignin
Phenolic metabolites
description 37 p.-6 fig.-2 tab.-1 graph. abst.
publishDate 2021
dc.date.none.fl_str_mv 2021
2021
2021
dc.type.none.fl_str_mv info:eu-repo/semantics/article
http://purl.org/coar/resource_type/c_6501
Preprint
info:eu-repo/semantics/submittedVersion
format article
status_str submittedVersion
dc.identifier.none.fl_str_mv http://hdl.handle.net/10261/253023
url http://hdl.handle.net/10261/253023
dc.language.none.fl_str_mv Inglés
language_invalid_str_mv Inglés
dc.relation.none.fl_str_mv https://doi.org/10.1016/j.biteb.2021.100742
Sí
dc.rights.none.fl_str_mv info:eu-repo/semantics/openAccess
eu_rights_str_mv openAccess
dc.publisher.none.fl_str_mv Elsevier
publisher.none.fl_str_mv Elsevier
dc.source.none.fl_str_mv reponame:DIGITAL.CSIC. Repositorio Institucional del CSIC
instname:Consejo Superior de Investigaciones Científicas (CSIC)
instname_str Consejo Superior de Investigaciones Científicas (CSIC)
reponame_str DIGITAL.CSIC. Repositorio Institucional del CSIC
collection DIGITAL.CSIC. Repositorio Institucional del CSIC
repository.name.fl_str_mv
repository.mail.fl_str_mv
_version_ 1869406980837212160
score 15,812455

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